Date published: 2025-9-8

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APC 抗体 (c-APC 28.9): sc-53166

4.4(5)
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说明书
  • APC抗体(c-APC 28.9)是小鼠单克隆IgG1 (kappa轻链),规格为200 µg/ml
  • 针对human C-terminal APC/MBP融合蛋白
  • 推荐用于 mouse, rat 和 human 来源的APC WB, IP, IF 和 IHC(P)检测
  • 看 APC (F-3): sc-9998,可查看有标记的 APC 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成APC 抗体 (c-APC 28.9)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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    APC抗体(c-APC 28.9)是一种小鼠单克隆IgG1抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学等方法检测小鼠、大鼠和人类样本中的APC。抗APC抗体(c-APC 28.9)靶向腺瘤性息肉病蛋白(APC),该蛋白在调节细胞粘附和维持细胞连接完整性方面起着至关重要的作用。APC(c-APC 28.9)单克隆抗体可识别由2843个氨基酸组成的APC蛋白,该蛋白对于Wnt信号传导通路发挥正常功能至关重要,对细胞增殖和分化也至关重要。APC基因突变通常与家族性腺瘤性息肉病和加德纳综合征有关,可导致大量腺瘤性息肉,并可能发展为结肠癌。APC与β-catenin和γ-catenin相互作用,有助于调节细胞粘附,并参与粘附连接的形成。APC与E-cadherin竞争与β-和γ-catenin的结合,这种动态维持细胞结构和信号传导。α-catenin介导细胞骨架与APC-E-cadherin-β/γ-catenin复合物之间的相互作用,突出了APC在细胞结构和功能中的重要性。

    仅限研究使用。不适用于诊断和治疗用途。

    订购信息

    产品名称产品编号规格价格数量收藏夹

    APC 抗体 (c-APC 28.9)

    sc-53166
    200 µg/ml
    RMB2377.00

    I am using APC (c-APC 28.9): sc-53166 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

    Asked by: Professor Griffin
    We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-02-24
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    Rated 5 out of 5 by from No problemAfter expose I got white bands on membrance (1:200 diluted the antibody),but at the target band position,this is unusual.I have searched the problem solutions, it's due to the high conceration antibody.I decreased the primary antbody conceration to 1:800 and the secondary antibody to 1:5000,I got black band.
    Date published: 2017-07-22
    Rated 5 out of 5 by from specific bandsI got strong specific bands with zero background in the positive control lanes,the antibody incubated condition can either be 4℃ overnight or RT 2hour.
    Date published: 2017-07-15
    Rated 4 out of 5 by from It works well!It works wll in human colon cancer cell lines KM12C and KM12SM, the band of target band APC was easy to detect, and was single, low background, and could be used repeatedly.
    Date published: 2016-12-26
    Rated 4 out of 5 by from Robust signal in WB in HeLa whole cell lysateRobust signal in WB in HeLa whole cell lysate. -SCBT QC
    Date published: 2015-04-17
    Rated 4 out of 5 by from Produced robust immunoperoxidase cytoplasmicProduced robust immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human esophagus tissue. -SCBT QC
    Date published: 2015-03-06
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    APC 抗体 (c-APC 28.9) is rated 4.4 out of 5 by 5.
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