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Apaf-1 Antibody (5E11) - Western Blotting - Image 150332
Apaf-1 抗体 (5E11): sc-65891. Jurkat 全细胞裂解液中 Apaf-1 表达的 Western 印迹分析.
Apaf-1 Antibody (5E11) - Western Blotting - Image 150332
Apaf-1 抗体 (5E11): sc-65891. Jurkat 全细胞裂解液中 Apaf-1 表达的 Western 印迹分析.
Apaf-1 抗体 (5E11): sc-65891. Jurkat 全细胞裂解液中 Apaf-1 表达的 Western 印迹分析.

Apaf-1 抗体 (5E11): sc-65891

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说明书
  • Apaf-1抗体(5E11)是小鼠单克隆IgG1 κ, 在22篇文献中引用，规格为50 µg/0.5 ml
  • 与氨基酸序列C-terminus（h）对应的多肽序列（Apaf1的human起源）
  • 推荐用于 mouse, rat 和 human 来源的Apaf-1 WB, IP, IF 和 IHC(P)检测
  • 关于如何获取Apaf-1 (5E11): sc-65891的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Apaf-1 抗体 (5E11)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

Apaf-1抗体（5E11）是一种IgG1 κ小鼠单克隆Apaf-1抗体，可通过WB、IP、IF和IHC(P)检测到小鼠、大鼠和人类来源的Apaf-1。Apaf-1抗体（5E11）为非偶联型抗Apaf-1抗体。Ced-4蛋白的哺乳动物同源物Apaf-1（Ced-4）、Nod1（CARD4）和Nod2包含一个半胱天冬酶募集结构域（CARD）和一个推定的核苷酸结合结构域，后者由共识Walker A盒（P-loop）和B盒（Mg2+-结合位点）表示。Nod1包含一个推定的调节结构域和多个富含亮氨酸的重复序列。Nod1是细胞内蛋白质家族中不断增长的一个成员，与凋亡调节因子Apaf-1具有结构同源性。Nod1与含CARD的激酶RICK结合并激活NFkB。Nod1的自我结合介导RICK的邻近和RICK与IKKg的相互作用。此外，Nod-1与多个具有长原域的半胱天冬酶结合，但特异性激活半胱天冬酶-9并促进半胱天冬酶-9诱导的凋亡。Nod2由两个N端CARD、一个核苷酸结合结构域和多个C端富含亮氨酸的重复序列组成。Nod2的表达高度局限于单核细胞，并响应细菌脂多糖激活NFkB。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Apaf-1 抗体 (5E11) 参考文献:

  1. 人类 CARD4 蛋白是一种新型 CED-4/Apaf-1 细胞死亡家族成员，可激活 NF-kappaB。  |  Bertin, J., et al. 1999. J Biol Chem. 274: 12955-8. PMID: 10224040
  2. Nod1，一种类似 Apaf-1 的 Caspase-9 和核因子-kappaB 激活剂。  |  Inohara, N., et al. 1999. J Biol Chem. 274: 14560-7. PMID: 10329646
  3. NF-kappa B 在 Nod1/RICK 和 RIP 信号通路中激活的诱导接近模型。  |  Inohara, N., et al. 2000. J Biol Chem. 275: 27823-31. PMID: 10880512
  4. 人类 Nod1 能增强对细菌脂多糖的反应能力  |  Inohara, N., et al. 2001. J Biol Chem. 276: 2551-4. PMID: 11058605
  5. 哺乳动物 Ced-4 细胞凋亡蛋白家族新成员 DEFCAP-L 和 -S 的两种同工型的分子克隆和表征。  |  Hlaing, T., et al. 2001. J Biol Chem. 276: 9230-8. PMID: 11076957
  6. 黑色素瘤中 Apaf-1 是否经常表达减弱？  |  Allen, JD., et al. 2005. Cell Death Differ. 12: 680-1. PMID: 15832174
  7. 黑色素瘤中细胞凋亡效应因子 Apaf-1 的表达和功能。  |  Peltenburg, LT., et al. 2005. Cell Death Differ. 12: 678-9. PMID: 15832175
  8. 宫颈癌中 Apaf-1 的表达与淋巴结转移有关，但与瘤内缺氧无关。  |  Leo, C., et al. 2005. Gynecol Oncol. 97: 602-6. PMID: 15863166
  9. Apaf-1：细胞死亡的调控和功能。  |  Shakeri, R., et al. 2017. Biochimie. 135: 111-125. PMID: 28192157
  10. Apaf-1 Pyroptosome感知线粒体渗透性转换  |  Xu, W., et al. 2021. Cell Metab. 33: 424-436.e10. PMID: 33308446
  11. Apaf-1对癌症和神经退行性疾病发病机制的贡献。  |  Shakeri, R., et al. 2021. Biochimie. 190: 91-110. PMID: 34298080
  12. Nod2 是一种 Nod1/Apaf-1 家族成员，仅限于单核细胞，能激活 NF-kappaB。  |  Ogura, Y., et al. 2001. J Biol Chem. 276: 4812-8. PMID: 11087742

订购信息

产品名称产品编号规格价格数量收藏夹

Apaf-1 抗体 (5E11)

sc-65891
50 µg/0.5 ml
RMB2377.00

It would be great if you could provide the information about the antigen of this antibody.

Asked by: JLOJ
Thank you for your question, there is no specific table position information for this antibody. Only the following information can be provided:raised against a peptide corresponding to amino acids near the C-terminus of Apaf1 of human origin.Thank you.
Answered by: Jiajun Z
Date published: 2024-04-12

APAf1 antibody(SC-65891), How long I can get it if I order it？ we have orderred it on Dec 14 from UPenn, but now I still do not get it.

Asked by: Shiying
Thank you for your question. Please contact technical service for further assistance.
Answered by: Technical Service 4
Date published: 2020-11-12

I want to use Apaf-1 (5E11): sc-65891for Western (inmuno) Blotting. In the technical sheet it is stated that the starting dilution is 1:200. Does this mean that to work on a 1:1000 dilution I only need to dilute it 5 times? (1:200 --> X5 =1:1000). Thanks

Asked by: Apaf lover
Thank you for your question.Apaf-1 Antibody (5E11) is a mouse monoclonal IgG1 provided at 50 µg in 0.5 ml. We recommend a starting dilution of 1:200 for Western blot. The expected range, to determined by each end-user, is 1:100-1:1,000.
Answered by: Technical Support
Date published: 2020-11-16

What is the recommended storage condition for Apaf-1 (5E11): sc-65891?

Asked by: Professor Griffin
Thank you for your question. We recommend storing the antibody at 4° C.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from Good choice for Immunoperoxidase stainingThis antibody has been used for Immunoperoxidase staining in published research. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from This antibody was used in a publication forThis antibody was used in a publication for WB on human gastric cancer cell line HGC-27 - SCBT Publication Review
Date published: 2015-03-25
Rated 5 out of 5 by from Single strong band at expected size usingSingle strong band at expected size using Jurkat whole cell lysate. -SCBT QC
Date published: 2015-01-24
Rated 5 out of 5 by from WB publishable data with HeLaWB publishable data with HeLa, MCF-7 and SW613-B3 - SCBT Publication Review
Date published: 2014-01-13
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Apaf-1 抗体 (5E11) is rated 5.0 out of 5 by 4.
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