Date published: 2025-9-8
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ANO1 Antibody (C-5) - Western Blotting - Image 281637
ANO1 抗体 (C-5): sc-377115. 人骨骼肌 (A) 和人肝脏 (B) 组织提取物中 ANO1 表达的 Western 印迹分析.
ANO1 抗体 (C-5): sc-377115. 人骨骼肌 (A) 和人肝脏 (B) 组织提取物中 ANO1 表达的 Western 印迹分析.

ANO1 抗体 (C-5): sc-377115

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说明书
  • ANO1 抗体 C-5 是小鼠单克隆 IgG2b κ，ANO1抗体, 在10篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列182-222位于human物种的ANO1的内部区域内
  • ANO1 抗体 (C-5) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 ANO1
  • 抗ANO1抗体（C-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ANO1 (C-5): sc-377115的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 ANO1 抗体 (C-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ANO1 抗体 (C-5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 ANO 抗体包括 ANO1 和 ANO2

ANO1抗体（C-5）是一种IgG2b κ小鼠单克隆ANO1抗体（也称为TAOS2抗体、DOG1抗体、在胃肠道间质瘤上发现的蛋白质1抗体、Anoctamin 1钙激活氯通道抗体、肿瘤扩增和过表达序列2抗体、口腔癌过表达2抗体、跨膜蛋白16A抗体、TMEM16A抗体、ORAOV2抗体，或跨膜蛋白16A（八个跨膜结构域）抗体），可通过WB、IP、IF、IHC(P)和ELISA检测人源性ANO1蛋白。ANO1抗体（C-5）既有非偶联型抗ANO1抗体形式，也有多种偶联型抗ANO1抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。ANO1（Anoctamin 1），也称为DOG1、ORAOV2、TAOS2或TMEM16A，是一种由986个氨基酸组成的多通道膜蛋白，定位于细胞膜和细胞质，属于Anoctamin家族。ANO1在多种组织中表达，在肝、胃肠道肌肉和骨骼肌中表达最高，作为钙激活的氯通道，对气管的正常发育至关重要。人类ANO1与其小鼠同源物的序列一致性为90%，表明其在物种间具有保守作用。ANO1存在于乳腺癌、胰腺癌、胃癌和子宫癌，以及颈部、卵巢和甲状旁腺肿瘤中，表明ANO1在致癌过程中发挥作用。由于选择性剪接事件，ANO1存在三种异构体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ANO1 抗体 (C-5) 参考文献:

  1. FLJ10261 基因位于人类染色体 11q13 上的 CCND1-EMS1 基因座内，编码与 C12orf3, C11orf25 和 FLJ34272 基因产物同源的八跨膜蛋白。  |  Katoh, M. and Katoh, M. 2003. Int J Oncol. 22: 1375-81. PMID: 12739008
  2. TMEM16E 和 TMEM16F 基因的鉴定和特征描述。  |  Katoh, M. and Katoh, M. 2004. Int J Oncol. 24: 1345-9. PMID: 15067359
  3. 通过全面有效的差异显示分析头颈部鳞状细胞癌转录组。  |  Carles, A., et al. 2006. Oncogene. 25: 1821-31. PMID: 16261155
  4. 人类口腔癌中 11q13 扩增片段的基因组和转录组综合分析，以及与小鼠口腔癌中 7F5 扩增片段的同源关系。  |  Huang, X., et al. 2006. Genes Chromosomes Cancer. 45: 1058-69. PMID: 16906560
  5. 常染色体隐性非综合征听力损伤的新基因座 DFNB63 映射到染色体 11q13.2-q13.4 上。  |  Kalay, E., et al. 2007. J Mol Med (Berl). 85: 397-404. PMID: 17211611
  6. TMEM16A 赋予受体激活的钙依赖性氯传导性。  |  Yang, YD., et al. 2008. Nature. 455: 1210-5. PMID: 18724360
  7. TMEM16A蛋白：Ca（2+）依赖性Cl-通道的新身份。  |  Ferrera, L., et al. 2010. Physiology (Bethesda). 25: 357-63. PMID: 21186280
  8. anoctamin 家族：作为钙激活氯离子通道的 TMEM16A 和 TMEM16B。  |  Scudieri, P., et al. 2012. Exp Physiol. 97: 177-83. PMID: 21984732
  9. 无钙通道是 Ca2+ 激活的 Cl- 通道家族。  |  Tian, Y., et al. 2012. J Cell Sci. 125: 4991-8. PMID: 22946059
  10. 葡萄糖诱导的膜电位振荡和小鼠 β 细胞分泌胰岛素需要 Anoctamin 1 (Ano1)。  |  Crutzen, R., et al. 2016. Pflugers Arch. 468: 573-91. PMID: 26582426
  11. miR-106a靶向Anoctamin 1（ANO1）调节巨噬细胞中脂多糖（LPS）诱导的炎症反应。  |  Heng, J., et al. 2020. Med Sci Monit. 26: e922479. PMID: 33037174
  12. ANO1介导的癌症铁死亡抑制通过招募癌相关成纤维细胞而产生免疫治疗耐药性。  |  Jiang, F., et al. 2023. Adv Sci (Weinh). 10: e2300881. PMID: 37341301

订购信息

产品名称产品编号规格价格数量收藏夹

ANO1 抗体 (C-5)

sc-377115
200 µg/ml
RMB2377.00

ANO1 (C-5): m-IgGκ BP-HRP 套装

sc-523193
200 µg Ab, 40 µg BP
RMB2662.00

ANO1 (C-5): m-IgG2b BP-HRP 套装

sc-549094
200 µg Ab; 10 µg BP
RMB2662.00

ANO1 抗体 (C-5) AC

sc-377115 AC
500 µg/ml, 25% agarose
RMB3129.00

ANO1 抗体 (C-5) HRP

sc-377115 HRP
200 µg/ml
RMB2377.00

ANO1 抗体 (C-5) FITC

sc-377115 FITC
200 µg/ml
RMB2482.00

ANO1 抗体 (C-5) PE

sc-377115 PE
200 µg/ml
RMB2580.00

ANO1 抗体 (C-5) Alexa Fluor® 488

sc-377115 AF488
200 µg/ml
RMB2685.00

ANO1 抗体 (C-5) Alexa Fluor® 546

sc-377115 AF546
200 µg/ml
RMB2685.00

ANO1 抗体 (C-5) Alexa Fluor® 594

sc-377115 AF594
200 µg/ml
RMB2685.00

ANO1 抗体 (C-5) Alexa Fluor® 647

sc-377115 AF647
200 µg/ml
RMB2685.00

ANO1 抗体 (C-5) Alexa Fluor® 680

sc-377115 AF680
200 µg/ml
RMB2685.00

ANO1 抗体 (C-5) Alexa Fluor® 790

sc-377115 AF790
200 µg/ml
RMB2685.00

How soon are you able to ship ANO1 Antibody (C-5): sc-377115?

Asked by: Allan
Thank you for contacting Santa Cruz Biotechnology. Please email [email protected] for a price quote and ship date .
Answered by: BlakeJ
Date published: 2024-06-13

I am using ANO1 (C-5): sc-377115 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from ANO1 antibody (C5) from Santa Cruz BiotechnologyThese antibodies show a band of a wrong molecular weight on Western blotting. Human ANO1 cannot be below 90 kDa - it should be around 140 kDa.
Date published: 2019-04-03
Rated 1 out of 5 by from ANO1 Antibody (C-5)Wrong molecular weight of ANO1 on Westerns.It cannot be 90K.
Date published: 2019-04-03
Rated 5 out of 5 by from Very good for immunofluorescenceI tested this antibody in immunofluorescence using 2% PFA-fixed and permeabilized (0.3% TritonX-100) cells that express high endogenous levels of ANO1 and it worked very well at 1:100.
Date published: 2017-09-26
Rated 4 out of 5 by from Good staining in Western BlotStaining of human kidney cells showed a very strong and unique band at the expected size.
Date published: 2017-07-03
Rated 3 out of 5 by from Hasn't worked...yet....I purchased this antibody for western blotting. So far it hasn't worked, but I hope to optimize the conditions and get it working...
Date published: 2017-02-24
Rated 5 out of 5 by from Good choice for IHCSpecific membrane and cytoplasmic staining of glandular cells seen in IHC with paraffin-embedded tissue.
Date published: 2017-01-23
Rated 5 out of 5 by from It works well on human vascular smooth muscleIt works well on human vascular smooth muscle cells by WB, IF, IP.
Date published: 2015-09-30
Rated 5 out of 5 by from Excellent immunoperoxidase membrane and cytoplasmicExcellent immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human gall bladder tissue. -SCBT QC
Date published: 2015-02-21
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ANO1 抗体 (C-5) is rated 3.8 out of 5 by 9.
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