Date published: 2025-9-8
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Annexin VI Antibody (E-5) - Western Blotting - Image 390787
Annexin VI 抗体 (E-5) Alexa Fluor® 546: sc-271859 AF546. MES-SA/Dx5 (A), Raji (B), CCRF-CEM (C) 和 U-937 (F) 全细胞裂解液以及人小脑 (D) 和人淋巴结 (E) 组织提取物中 Annexin VI 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.
Annexin VI 抗体 (E-5) Alexa Fluor® 546: sc-271859 AF546. MES-SA/Dx5 (A), Raji (B), CCRF-CEM (C) 和 U-937 (F) 全细胞裂解液以及人小脑 (D) 和人淋巴结 (E) 组织提取物中 Annexin VI 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断.

Annexin VI 抗体 (E-5): sc-271859

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说明书
  • Annexin VI 抗体 E-5 是小鼠单克隆 IgG1 κ，Annexin VI抗体, 在9篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Annexin VI的N-terminus的氨基酸5-27之间
  • Annexin VI 抗体 (E-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Annexin VI
  • 抗Annexin VI抗体（E-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Annexin VI (E-5): sc-271859的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是Annexin VI Antibody (E-5) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现可与Annexin VI Antibody (E-5) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Annexin抗体，包括Annexin A10, Annexin A13, Annexin A9, Annexin I, Annexin II, Annexin III, Annexin IV, Annexin V, Annexin VI, Annexin VII, Annexin VIII和Annexin XI

Annexin VI 抗体（E-5）是一种 IgG1 κ 鼠单克隆 Annexin VI 抗体（也称为 ANXA6 抗体、Annexin A6 抗体、Chromobindin-20 抗体、Lipocortin VI 抗体、Calphobindin II 抗体或 67 kDa calelectrin 抗体），可通过 WB、IP、IF、IHC(P) 和 ELISA 检测小鼠、大鼠和人源性的 Annexin VI 蛋白。Annexin VI 抗体（E-5）既可以是非偶联的抗 Annexin VI 抗体形式，也可以是多种偶联形式的抗 Annexin VI 抗体，包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。钙结合蛋白的膜联蛋白家族至少由十个哺乳动物基因组成。它的特点是一个保守的核心结构域，该结构域以 Ca++-依赖的方式与磷脂结合，以及一个独特的氨基末端区域，该区域可能赋予结合特异性。膜联蛋白家族已被牵涉为离子通量、内吞和外排以及细胞粘附等多种过程的调节剂。当在 A-431 细胞中过度表达时，Annexin VI 会导致转化表型的部分逆转。有人假设，膜联蛋白的生长依赖性翻译后修饰对于其正确的亚细胞定位是必需的。膜联蛋白 VII，也称为突触融合蛋白，位于正常肌肉组织的质膜上。然而，在来自杜氏肌营养不良患者的肌肉样本中，膜联蛋白 VII 与膜联蛋白 IV 和 VI 一起被释放到细胞质中，随后随着疾病的进展进入细胞外空间。已经鉴定出两种形式的膜联蛋白 XI，分别称为 A 和 B。用膜联蛋白 XI-A 转染 COS-7 细胞，而非膜联蛋白 XI-B，会导致形成与膜联蛋白 XI 相关的囊泡。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Annexin VI 抗体 (E-5) 参考文献:

  1. Annexin VI 在平滑肌细胞中参与形成可逆的膜-骨架复合体。  |  Babiychuk, EB., et al. 1999. J Biol Chem. 274: 35191-5. PMID: 10575003
  2. 鉴定胰腺尖腺细胞中作为 Ca2+ 敏感 CRHSP-28 结合蛋白的附件蛋白 VI。  |  Thomas, DD., et al. 2002. J Biol Chem. 277: 35496-502. PMID: 12105190
  3. 附件素 VI 的一种依赖于生长的翻译后修饰。  |  Moss, SE., et al. 1992. Biochim Biophys Acta. 1160: 120-6. PMID: 1420329
  4. 大鼠门齿分泌性成骨细胞和牙本质细胞中附件蛋白 VI 和肌动蛋白的共同分布。  |  Goldberg, M., et al. 1991. Cell Tissue Res. 263: 81-9. PMID: 1826233
  5. 附件蛋白超级基因家族的结构演变。  |  Smith, PD. and Moss, SE. 1994. Trends Genet. 10: 241-6. PMID: 8091504
  6. 肌营养不良导致的 Annexin VII 重定位  |  Selbert, S., et al. 1996. Exp Cell Res. 222: 199-208. PMID: 8549664
  7. S100C 与附件蛋白 I N 端结构域的钙依赖性结合。  |  Mailliard, WS., et al. 1996. J Biol Chem. 271: 719-25. PMID: 8557678
  8. 附件蛋白 VI 的功能和遗传分析。  |  Edwards, HC. and Moss, SE. 1995. Mol Cell Biochem. 149-150: 293-9. PMID: 8569744
  9. Annexin II 四聚体：结构与功能。  |  Waisman, DM. 1995. Mol Cell Biochem. 149-150: 301-22. PMID: 8569746
  10. 外泌过程中的附蛋白 II：儿茶酚胺的分泌需要 p36 转位至绒毛膜细胞的浆膜下区域。  |  Chasserot-Golaz, S., et al. 1996. J Cell Biol. 133: 1217-36. PMID: 8682860
  11. 重组附件素 XI-A 在 Sf9 细胞中形成的细胞内囊泡具有同工酶特异性。  |  Sudo, T., et al. 1996. Biochem Biophys Res Commun. 223: 706-11. PMID: 8687461
  12. Annexin VI 可调节脊髓和背根神经节神经元的 Ca2+ 和 K+ 传导。  |  Naciff, JM., et al. 1996. Am J Physiol. 271: C2004-15. PMID: 8997203

订购信息

产品名称产品编号规格价格数量收藏夹

Annexin VI 抗体 (E-5)

sc-271859
200 µg/ml
RMB2377.00

Annexin VI (E-5): m-IgGκ BP-HRP 套装

sc-522080
200 µg Ab, 40 µg BP
RMB2662.00

Annexin VI 抗体 (E-5) AC

sc-271859 AC
500 µg/ml, 25% agarose
RMB3129.00

Annexin VI 抗体 (E-5) HRP

sc-271859 HRP
200 µg/ml
RMB2377.00

Annexin VI 抗体 (E-5) FITC

sc-271859 FITC
200 µg/ml
RMB2482.00

Annexin VI 抗体 (E-5) PE

sc-271859 PE
200 µg/ml
RMB2580.00

Annexin VI 抗体 (E-5) Alexa Fluor® 488

sc-271859 AF488
200 µg/ml
RMB2685.00

Annexin VI 抗体 (E-5) Alexa Fluor® 546

sc-271859 AF546
200 µg/ml
RMB2685.00

Annexin VI 抗体 (E-5) Alexa Fluor® 594

sc-271859 AF594
200 µg/ml
RMB2685.00

Annexin VI 抗体 (E-5) Alexa Fluor® 647

sc-271859 AF647
200 µg/ml
RMB2685.00

Annexin VI 抗体 (E-5) Alexa Fluor® 680

sc-271859 AF680
200 µg/ml
RMB2685.00

Annexin VI 抗体 (E-5) Alexa Fluor® 790

sc-271859 AF790
200 µg/ml
RMB2685.00

Annexin VI (E-5) 中和勝肽

sc-271859 P
100 µg/0.5 ml
RMB511.00

I'm interested in testing this (sc-271859 ) antibody on zebrafish and would like to ask if it is possible to get a test sample?

Asked by: Alexandra73
Thank you for your question. Please contact our technical service team to arrange for a free sample of this antibody.
Answered by: Tech Support Europe
Date published: 2022-03-25

What application is the blocking peptide sc-271859 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from 功能很出色我实验期间多次购买了该产品，对产品性能很满意,所以本次我希望能收到贵公司赠送的Annexin VI抗体 (E-5)小样
Date published: 2023-08-14
Rated 5 out of 5 by from Ottimo funzionamento!!!L’anticorpo anti-Annexin VI (sc-271859), richiesto con l’Ordine n° 535 dell’11/11/2021 e arrivato il giorno 23/11/2021, è stato testato su un lisato di cellule HeLa, ottenuto tramite omogenizzazione manuale in una soluzione di PBS-0.1% Igepal contenente un cocktail di inibitori di proteasi. Aliquote di lisato da 2 μg, 5 μg, 10 μg e 20 μg di proteine sono state caricate su un gel di poliacrilammide al 10% e sottoposte a Western Blotting. La membrana così ottenuta è stata sottoposta a blocking (in una soluzione al 5% di non-fat dried milk in TBS-0.05% TWEEN 20) e, successivamente, incubata con l’anticorpo primario sopra riportato (1:1000 vol/vol). La membrana è poi stata incubata con un anticorpo secondario anti-mouse (1:1500 vol/vol) coniugato con perossidasi di rafano. I segnali di chemiluminescenza sono stati rivelati tramite il sistema di imaging digitale Amersham Imager 680 (GE Healthcare Life Sciences). Come mostrato nell’immagine di seguito riportata, l’anticorpo produce segnali in corrispondenza del peso molecolare dell’Annessina VI in maniera concentrazione dipendente.
Date published: 2021-11-25
Rated 5 out of 5 by from This antibody is good for WB and IPI got this antibody few month before, it is good for both WB and IP
Date published: 2017-11-20
Rated 5 out of 5 by from works great on western blot and IHCWe have used this antibody on human skin tissue. It works great on both western blot and IHC.
Date published: 2017-09-20
Rated 4 out of 5 by from WORKED WELLI received this antibody as a free trial sample and used for WB and Immunoprecipitation. It worked well.
Date published: 2017-09-11
Rated 5 out of 5 by from Distinct band observed via WB using product scDistinct band observed via WB using product sc-271859 using HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-12
Rated 5 out of 5 by from Good specificity shown by using transfectedGood specificity shown by using transfected and non-transfected 293T cells for over-expression of the human Annexin VIII protein via Western Blot analysis. -SCBT QC
Date published: 2015-03-29
Rated 5 out of 5 by from Positive detection of endogenous AnnexinPositive detection of endogenous Annexin VIII in JAR cells by WB analysis. -SCBT QC
Date published: 2015-03-21
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Annexin VI 抗体 (E-5) is rated 4.9 out of 5 by 9.
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