Date published: 2025-9-7
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Anillin Antibody (B-10) - Western Blotting - Image 382601
Anillin 抗体 (B-10): sc-271814. NIH/3T3 (A), DU 145 (B) 和 c4 (C) 全细胞裂解液中 Anillin 表达的 Western 印迹分析.
Anillin 抗体 (B-10): sc-271814. NIH/3T3 (A), DU 145 (B) 和 c4 (C) 全细胞裂解液中 Anillin 表达的 Western 印迹分析.

Anillin 抗体 (B-10): sc-271814

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说明书
  • Anillin 抗体 B-10 是小鼠单克隆 IgG2b κ，Anillin抗体, 在9篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列641-940位于human物种的Anillin的C-terminus附近
  • Anillin 抗体 (B-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Anillin
  • 抗Anillin抗体（B-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Anillin (B-10): sc-271814的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 Anillin 抗体 (B-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Anillin 抗体 (B-10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Anillin抗体（B-10）是一种IgG2b κ小鼠单克隆Anillin抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的Anillin蛋白。Anillin抗体（B-10）既可以是非偶联的抗Anillin抗体形式，也可以是多种偶联形式的抗Anillin抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Anillin，也称为scraps同源物，是一种进化上保守的肌动蛋白结合蛋白，对于细胞分裂是必需的，最初在果蝇中被发现。Anillin是一种普遍表达的蛋白质，在中枢神经系统中的表达水平最高。它主要存在于细胞核中，在细胞分裂期间定位于分裂沟，并在Rac GTPase的帮助下形成环状结构。在细胞分裂期间，Anillin与CD2AP相互作用，并起到集中Rho A和维持活性肌球蛋白定位的作用。在Anillin敲除细胞中，分裂沟无法完成内陷。Anillin表达水平随细胞周期波动，在有丝分裂期达到高峰。在细胞进入G1期之前，Anillin与E-钙粘蛋白结合并被后期促进复合物/细胞周期体（APC/C）泛素化。APC/C识别Anillin N端区域的D-box结构域。Anillin通常在肿瘤中过度表达，并可能作为潜在的生物标志物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Anillin 抗体 (B-10) 参考文献:

  1. 用小干扰 RNA 消减 PRC1 表明，在哺乳动物细胞中，细胞运动的脱落需要中央纺锤体束，而完成沟裂则不需要。  |  Mollinari, C., et al. 2005. Mol Biol Cell. 16: 1043-55. PMID: 15616196
  2. CD2 相关蛋白参与细胞分裂的线索  |  Monzo, P., et al. 2005. Mol Biol Cell. 16: 2891-902. PMID: 15800069
  3. Anillin 是无性繁殖促进复合体/环体（APC/C）的底物，在细胞分裂后期控制肌球蛋白的空间收缩性。  |  Zhao, WM. and Fang, G. 2005. J Biol Chem. 280: 33516-24. PMID: 16040610
  4. MgcRacGAP 控制着收缩环的组装和细胞分裂的启动。  |  Zhao, WM. and Fang, G. 2005. Proc Natl Acad Sci U S A. 102: 13158-63. PMID: 16129829
  5. 胰岛素结合蛋白anillin在多种人类肿瘤中过度表达。  |  Hall, PA., et al. 2005. Clin Cancer Res. 11: 6780-6. PMID: 16203764
  6. ANLN 通过激活 RHOA 和参与磷酸肌醇 3- 激酶/AKT 通路，在人类肺癌发生过程中发挥着关键作用。  |  Suzuki, C., et al. 2005. Cancer Res. 65: 11314-25. PMID: 16357138
  7. 振动器编码的果蝇磷脂酰肌醇转移蛋白对维持细胞分裂过程中的裂殖匍匐至关重要。  |  Gatt, MK. and Glover, DM. 2006. J Cell Sci. 119: 2225-35. PMID: 16684816
  8. 心肌细胞双核中的 Anillin 定位缺陷  |  Engel, FB., et al. 2006. J Mol Cell Cardiol. 41: 601-12. PMID: 16889791
  9. Anillin 和 septins 可促进细胞动因沟的非对称摄取。  |  Maddox, AS., et al. 2007. Dev Cell. 12: 827-35. PMID: 17488632

订购信息

产品名称产品编号规格价格数量收藏夹

Anillin 抗体 (B-10)

sc-271814
200 µg/ml
RMB2377.00

Anillin (B-10): m-IgGκ BP-HRP 套装

sc-522069
200 µg Ab, 40 µg BP
RMB2662.00

Anillin (B-10): m-IgG2b BP-HRP 套装

sc-549001
200 µg Ab; 10 µg BP
RMB2662.00

Anillin 抗体 (B-10) AC

sc-271814 AC
500 µg/ml, 25% agarose
RMB3129.00

Anillin 抗体 (B-10) HRP

sc-271814 HRP
200 µg/ml
RMB2377.00

Anillin 抗体 (B-10) FITC

sc-271814 FITC
200 µg/ml
RMB2482.00

Anillin 抗体 (B-10) PE

sc-271814 PE
200 µg/ml
RMB2580.00

Anillin 抗体 (B-10) Alexa Fluor® 488

sc-271814 AF488
200 µg/ml
RMB2685.00

Anillin 抗体 (B-10) Alexa Fluor® 546

sc-271814 AF546
200 µg/ml
RMB2685.00

Anillin 抗体 (B-10) Alexa Fluor® 594

sc-271814 AF594
200 µg/ml
RMB2685.00

Anillin 抗体 (B-10) Alexa Fluor® 647

sc-271814 AF647
200 µg/ml
RMB2685.00

Anillin 抗体 (B-10) Alexa Fluor® 680

sc-271814 AF680
200 µg/ml
RMB2685.00

Anillin 抗体 (B-10) Alexa Fluor® 790

sc-271814 AF790
200 µg/ml
RMB2685.00

Can Anillin (B-10): sc-271814 monoclonal antibody be used to stain paraformaldehyde-fixed cancer cells for immunofluoresence microscopy? Thank you!

Asked by: Evangelos
Thank you for the question. We have not yet tested this antibody on pfa- fixed cells for IF. Instead we suggest using cells fixed with cold methanol according to our protocols at a starting dilution of 1:50. Since this antibody is working well in IHC(P) on formalin-fixed, paraffin-embedded tissue sections, it is not unlikely this clone can also be used to stain paraformaldehyde-fixed cancer cells for immunofluoresence microscopy. If you have further questions, please contact our Technical Service Dept.
Answered by: Technical Support Europe
Date published: 2024-01-03

Can Anillin (B-10): sc-271814 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Dr Ninau Qelp
Thank you for your question. Yes, Anillin (B-10): sc-271814 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Efficient WB antibodyI got a free aliquot of this antibody and tested it on HEK293 samples transfected with siCo or siANLN. The antibody showed high specificity with no background. I used it 1:1000 in 0,1% milk with O/N incubation at 4°C. I would recommend it.
Date published: 2021-04-19
Rated 5 out of 5 by from positive bandThis antibody gave a positive band when use it detect Anillin in mouse lung tissue, I suggest 1:500 for the WB analysis.
Date published: 2017-09-02
Rated 5 out of 5 by from Very nice qualityVery nice quality ,it shows clear strong signal in rat kidney tissue,I suggest 1:1000 diluted the antibody for WB,no background seen use 5% BSA buffer.
Date published: 2017-08-26
Rated 5 out of 5 by from Efficient antibodyIt's a very efficient antibody that works well for IHC detect mouse spleen tissue,1:500 diluted ,use the goat serum for blocking ,strong signal staining and no backgound.
Date published: 2017-08-26
Rated 5 out of 5 by from Very powerful antibodiesThis antibody shows good working for human heart tissue extract by WB with very no background,but strong bands.
Date published: 2017-04-29
Rated 5 out of 5 by from Nice wb dataNice data for WB studies,got clear band and no nonspecific bands.
Date published: 2017-02-12
Rated 5 out of 5 by from Good AntibodyAnillin antibody provides clear and specific results. Strong single band in WB in mouse peritoneal macrophages.
Date published: 2017-02-07
Rated 4 out of 5 by from good for immunoflorescenceReceived as a free sample to replace a goat antibody that did not stain cells
Date published: 2016-12-23
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Anillin 抗体 (B-10) is rated 4.9 out of 5 by 12.
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