Date published: 2025-9-7

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Angiomotin-L2 抗体 (A-9): sc-398261

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说明书
  • Angiomotin-L2 抗体 A-9 是小鼠单克隆 IgG1 κ,Angiomotin-L2抗体, 在1篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列595-634位于human物种的Angiomotin-L2的内部区域内
  • Angiomotin-L2 抗体 (A-9) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 Angiomotin-L2; 也和以下物种反应,包括: 和 canine and bovine
  • 抗Angiomotin-L2抗体(A-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Angiomotin-L2 (A-9): sc-398261免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是Angiomotin-L2 Antibody (A-9) for WB applications. 的首选辅助检测试剂,这些试剂现与Angiomotin-L2 Antibody (A-9) 打包提供(请参阅下面的订购信息)。

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関連項目

Angiomotin-L2抗体(A-9)是一种IgG1 κ小鼠单克隆Angiomotin-L2抗体(也被指定为AMOL2蛋白抗体、Angiomotin Like 2抗体、Leman Coiled-Coil蛋白抗体、Angiomotin-Like Protein 2抗体、LCCP抗体或AMOTL2抗体),它可以通过WB、IP、IF和ELISA方法检测人类来源的Angiomotin-L2蛋白。Angiomotin-L2抗体(A-9)既可以非偶联的抗Angiomotin-L2抗体形式提供,也可以多种偶联形式的抗Angiomotin-L2抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Angiomotin,也被称为AMOT,是一种由1084个氨基酸组成的蛋白质,属于血管抑素结合蛋白的motin家族。motin家族的成员在其C端含有保守的卷曲螺旋结构域和PDZ结合基序。Angiomotin在骨骼肌和胎盘中表达,定位于细胞表面的紧密连接处,据信参与紧密连接的维持。Angiomotin与血管抑素结合,在血管生成中发挥重要作用,促进小管形成和生长因子诱导的内皮细胞迁移。这表明Angiomotin可能是肿瘤血管生成中的重要因子,并可能作为癌症的潜在治疗靶点。Angiomotin-L2,也被称为AMOTL2或LCCP,是一种由779个氨基酸组成的蛋白质,存在多种可选剪接的异构体,并可能以与Angiomotin相似的方式发挥作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Angiomotin-L2 抗体 (A-9) 参考文献:

  1. 预测人类不明基因的编码序列。XIV.100 个新的脑部 cDNA 克隆的完整序列,它们在体外编码大型蛋白质。  |  Kikuno, R., et al. 1999. DNA Res. 6: 197-205. PMID: 10470851
  2. Angiomotin:一种血管生长抑素结合蛋白,可调节内皮细胞迁移和管道形成。  |  Troyanovsky, B., et al. 2001. J Cell Biol. 152: 1247-54. PMID: 11257124
  3. Angiomotin 属于一个新的蛋白质家族,具有保守的线圈和 PDZ 结合结构域。  |  Bratt, A., et al. 2002. Gene. 298: 69-77. PMID: 12406577
  4. p130-angiomotin 与肌动蛋白结合并控制内皮细胞的形状。  |  Ernkvist, M., et al. 2006. FEBS J. 273: 2000-11. PMID: 16640563
  5. Rich1/Amot复合物调节上皮细胞中的Cdc42 GTP酶和顶端极性蛋白。  |  Wells, CD., et al. 2006. Cell. 125: 535-48. PMID: 16678097
  6. Amotl2对斑马鱼胚胎的细胞运动至关重要,并能调节c-Src的转位。  |  Huang, H., et al. 2007. Development. 134: 979-88. PMID: 17293535
  7. Angiomotin 在胚胎血管生成过程中调节内皮细胞迁移。  |  Aase, K., et al. 2007. Genes Dev. 21: 2055-68. PMID: 17699752
  8. 针对血管生长因子的治疗性抗体可抑制体内血管生成。  |  Levchenko, T., et al. 2008. FASEB J. 22: 880-9. PMID: 17984175
  9. p80- 和 p130- 血管球蛋白在内皮细胞迁移和稳定之间的转换中发挥不同作用。  |  Ernkvist, M., et al. 2008. Biochim Biophys Acta. 1783: 429-37. PMID: 18164266
  10. Angiomotin-like 2 单泛素化对 YAP 抑制的作用  |  Kim, M., et al. 2016. EMBO Rep. 17: 64-78. PMID: 26598551
  11. 类血管紧张素 2 与 AKT 相互作用并对其进行负向调节。  |  Han, H., et al. 2017. Oncogene. 36: 4662-4669. PMID: 28368415
  12. 血管动素样蛋白2上调通过抑制YAP1信号传导改善实验性肺动脉高血压。  |  Deng, J., et al. 2024. J Cardiovasc Pharmacol. 84: 356-369. PMID: 39027975

订购信息

产品名称产品编号规格价格数量收藏夹

Angiomotin-L2 抗体 (A-9)

sc-398261
200 µg/ml
RMB2377.00

Angiomotin-L2 (A-9): m-IgG Fc BP-HRP 套装

sc-527759
200 µg Ab; 10 µg BP
RMB2662.00

Angiomotin-L2 (A-9): m-IgG1 BP-HRP 套装

sc-533132
200 µg Ab; 20 µg BP
RMB2662.00

Angiomotin-L2 抗体 (A-9) AC

sc-398261 AC
500 µg/ml, 25% agarose
RMB3129.00

Angiomotin-L2 抗体 (A-9) HRP

sc-398261 HRP
200 µg/ml
RMB2377.00

Angiomotin-L2 抗体 (A-9) FITC

sc-398261 FITC
200 µg/ml
RMB2482.00

Angiomotin-L2 抗体 (A-9) PE

sc-398261 PE
200 µg/ml
RMB2580.00

Angiomotin-L2 抗体 (A-9) Alexa Fluor® 488

sc-398261 AF488
200 µg/ml
RMB2685.00

Angiomotin-L2 抗体 (A-9) Alexa Fluor® 546

sc-398261 AF546
200 µg/ml
RMB2685.00

Angiomotin-L2 抗体 (A-9) Alexa Fluor® 594

sc-398261 AF594
200 µg/ml
RMB2685.00

Angiomotin-L2 抗体 (A-9) Alexa Fluor® 647

sc-398261 AF647
200 µg/ml
RMB2685.00

Angiomotin-L2 抗体 (A-9) Alexa Fluor® 680

sc-398261 AF680
200 µg/ml
RMB2685.00

Angiomotin-L2 抗体 (A-9) Alexa Fluor® 790

sc-398261 AF790
200 µg/ml
RMB2685.00

Has this antibody been tested to work for IHC of paraffin embedded sections?

Asked by: customer who bought A9 Ab
Thank you for your question. This mouse monoclonal, Angiomotin-L2 Antibody (A-9): sc-398261, has not yet been tested on formalin-fixed paraffin-embedded tissue sections. Therefore, it is not recommended for IHC(P) at this time. We do recommend the antibody for IF, or IHC or IF on fresh or frozen tissue sections or cells fixed in methanol or acetone.
Answered by: Technical Support
Date published: 2017-04-13

What is the recommended starting dilution for Western blot for sc-398261: Angiomotin-L2 (A-9) monoclonal antibody?

Asked by: Professor Griffin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2021-10-31
  • y_2025, m_9, d_7, h_4CST
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Rated 1 out of 5 by from not good for WBI used this antibody according to the manufacturer's instructions on MCF10A and M2 cell lysates previously tested for AMOTL2 protein levels with other reagents. I didn't detect any signal
Date published: 2023-02-03
Rated 4 out of 5 by from not a sensitive antibodyI don't detect a clear band in 293A whole cell lysate when incubated the membrane with 1:500 dilution of primary antibody at 4°C overnight
Date published: 2017-07-04
Rated 5 out of 5 by from Nice Western blot data of AngiomotinNice Western blot data of Angiomotin-L2 expression in non-transfected and human Angiomotin-L2 transfected 293 whole cell lysates. -SCBT QC
Date published: 2013-11-22
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Angiomotin-L2 抗体 (A-9) is rated 3.3 out of 5 by 3.
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