Date published: 2025-9-7

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Angiomotin 抗体 (B-4): sc-166924

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说明书
  • Angiomotin 抗体 B-4 是小鼠单克隆 IgG2b κ,Angiomotin抗体, 在13篇文献中引用,规格为200 µg/ml
  • 针对氨基酸174-279的图谱,在Angiomotin的内部区域,human起源
  • Angiomotin 抗体 (B-4) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Angiomotin
  • 抗Angiomotin抗体(B-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Angiomotin (B-4): sc-166924免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP是Angiomotin Antibody (B-4) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Angiomotin Antibody (B-4) 打包提供(请参阅下面的订购信息)。

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関連項目

Angiomotin抗体(B-4)是一种IgG2b κ小鼠单克隆Angiomotin抗体(也称为Angiomotin P130亚型抗体或Angiomotin P80亚型抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的Angiomotin蛋白。Angiomotin抗体(B-4)既可以是非偶联的抗Angiomotin抗体形式,也可以是多种偶联形式的抗Angiomotin抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Angiomotin,也称为AMOT,是一种包含1084个氨基酸的蛋白质,属于血管抑素结合蛋白的motin家族。motin家族成员在其C端含有保守的卷曲螺旋结构域和PDZ结合基序。Angiomotin在骨骼肌和胎盘中表达,定位于细胞表面的紧密连接处,并被认为参与紧密连接的维持。Angiomotin与血管抑素结合,在血管生成中发挥重要作用,促进小管形成和生长因子诱导的内皮细胞迁移。这表明Angiomotin可能是肿瘤血管生成中的重要因子,并可作为癌症的潜在治疗靶点。由于选择性剪接事件,存在两种Angiomotin亚型,即p130和p80。p130亚型与p80亚型的表达模式不同,能够与F-肌动蛋白相互作用并诱导肌动蛋白纤维的形成。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Angiomotin 抗体 (B-4) 参考文献:

  1. 预测人类不明基因的编码序列。XIV.100 个新的脑部 cDNA 克隆的完整序列,它们在体外编码大型蛋白质。  |  Kikuno, R., et al. 1999. DNA Res. 6: 197-205. PMID: 10470851
  2. Angiomotin:一种血管生长抑素结合蛋白,可调节内皮细胞迁移和管道形成。  |  Troyanovsky, B., et al. 2001. J Cell Biol. 152: 1247-54. PMID: 11257124
  3. p130-angiomotin 与肌动蛋白结合并控制内皮细胞的形状。  |  Ernkvist, M., et al. 2006. FEBS J. 273: 2000-11. PMID: 16640563
  4. Rich1/Amot复合物调节上皮细胞中的Cdc42 GTP酶和顶端极性蛋白。  |  Wells, CD., et al. 2006. Cell. 125: 535-48. PMID: 16678097
  5. Amotl2对斑马鱼胚胎的细胞运动至关重要,并能调节c-Src的转位。  |  Huang, H., et al. 2007. Development. 134: 979-88. PMID: 17293535
  6. Angiomotin 在胚胎血管生成过程中调节内皮细胞迁移。  |  Aase, K., et al. 2007. Genes Dev. 21: 2055-68. PMID: 17699752
  7. 针对血管生长因子的治疗性抗体可抑制体内血管生成。  |  Levchenko, T., et al. 2008. FASEB J. 22: 880-9. PMID: 17984175
  8. p80- 和 p130- 血管球蛋白在内皮细胞迁移和稳定之间的转换中发挥不同作用。  |  Ernkvist, M., et al. 2008. Biochim Biophys Acta. 1783: 429-37. PMID: 18164266
  9. Angiomotin'g YAP 进入细胞核,促进细胞增殖和癌症发展。  |  Hong, W. 2013. Sci Signal. 6: pe27. PMID: 24003252
  10. 血管生长因子在神经系统中的新作用。  |  Wigerius, M., et al. 2020. Sci Signal. 13: PMID: 33109746
  11. DDI2 蛋白质溶解激活血管生成素,促进血管生成。  |  Wang, Y., et al. 2023. EMBO J. 42: e112900. PMID: 37350545
  12. Hippo 信号通路中的 Angiomotin 家族蛋白。  |  Wang, Y. and Yu, FX. 2024. Bioessays. 46: e2400076. PMID: 38760875

订购信息

产品名称产品编号规格价格数量收藏夹

Angiomotin 抗体 (B-4)

sc-166924
200 µg/ml
RMB2377.00

Angiomotin (B-4): m-IgG2b BP-HRP 套装

sc-548683
200 µg Ab; 10 µg BP
RMB2662.00

Angiomotin 抗体 (B-4) AC

sc-166924 AC
500 µg/ml, 25% agarose
RMB3129.00

Angiomotin 抗体 (B-4) HRP

sc-166924 HRP
200 µg/ml
RMB2377.00

Angiomotin 抗体 (B-4) FITC

sc-166924 FITC
200 µg/ml
RMB2482.00

Angiomotin 抗体 (B-4) PE

sc-166924 PE
200 µg/ml
RMB2580.00

Angiomotin 抗体 (B-4) Alexa Fluor® 488

sc-166924 AF488
200 µg/ml
RMB2685.00

Angiomotin 抗体 (B-4) Alexa Fluor® 546

sc-166924 AF546
200 µg/ml
RMB2685.00

Angiomotin 抗体 (B-4) Alexa Fluor® 594

sc-166924 AF594
200 µg/ml
RMB2685.00

Angiomotin 抗体 (B-4) Alexa Fluor® 647

sc-166924 AF647
200 µg/ml
RMB2685.00

Angiomotin 抗体 (B-4) Alexa Fluor® 680

sc-166924 AF680
200 µg/ml
RMB2685.00

Angiomotin 抗体 (B-4) Alexa Fluor® 790

sc-166924 AF790
200 µg/ml
RMB2685.00

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-166924 PE), fluorescein (sc-166924 FITC), Alexa Fluor® 488 (sc-166924 AF488) or Alexa Fluor® 647 (sc-166924 AF647. Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 10
Date published: 2017-01-17
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Rated 3 out of 5 by from Work specifically. Yet not so good.This anti-AMOT works with mouse preimplantation embryos in IF application (1:1000 dilutions). It is specific. However, it is not consistent in repeated trials or each embryo from the same batch of samples.
Date published: 2017-10-31
Rated 5 out of 5 by from Works well with human samplesI got a clear band in 293A whole cell lysate and it can also use for IP
Date published: 2017-07-04
Rated 5 out of 5 by from great features!I bought this several months ago and I‘m satisfied with this product. The results of experiments were clear and good.
Date published: 2017-06-01
Rated 5 out of 5 by from Good for WBAngiomotin Antibody (B-4) produced single strong band with little to no background
Date published: 2017-01-24
Rated 5 out of 5 by from Good Western Blot data of Angiomotin expressionGood Western Blot data of Angiomotin expression in 293T whole cell lysate. -SCBT QC
Date published: 2015-05-16
Rated 5 out of 5 by from Good Western Blot data of Angiomotin expressionGood Western Blot data of Angiomotin expression in SJRH30 whole cell lysate. -SCBT QC
Date published: 2014-12-13
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Angiomotin 抗体 (B-4) is rated 4.7 out of 5 by 6.
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