Date published: 2025-9-11
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Amylase Antibody (G-10) - Western Blotting - Image 10422
淀粉酶抗体 (G-10): sc-46657. 小鼠胰腺 (A) 和大鼠胰腺 (B) 组织提取物中淀粉酶表达的 Western 印迹分析.
淀粉酶抗体 (G-10): sc-46657. 小鼠胰腺 (A) 和大鼠胰腺 (B) 组织提取物中淀粉酶表达的 Western 印迹分析.

Amylase 抗体 (G-10): sc-46657

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说明书
  • Amylase 抗体 G-10 是小鼠单克隆 IgG2a κ，Amylase抗体, 在89篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Amylase 2B的氨基酸212-492
  • Amylase 抗体 (G-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Amylase 1A, 2A and 2B
  • 抗Amylase抗体（G-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Amylase (G-10): sc-46657的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 Amylase 抗体 (G-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Amylase 抗体 (G-10) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

淀粉酶抗体（G-10）是一种小鼠单克隆IgG2a kappa轻链抗体，用于检测多种物种（包括小鼠、大鼠和人类）的淀粉酶蛋白。抗淀粉酶抗体（G-10）靶向人类淀粉酶2B（也称为AMY2B）的氨基酸 212-492，也称为AMY2B，可有效识别淀粉酶1A（AMY1A）和淀粉酶2A（AMY2A）等关键同工型。淀粉酶在碳水化合物代谢中起着关键作用，它催化淀粉分解为更简单的糖，这对于正常的消化功能和能量产生至关重要。了解淀粉酶的表达和调节对于研究消化系统疾病、胰腺健康和某些卵巢肿瘤至关重要，因此淀粉酶（G-10）抗体成为这些研究中宝贵的工具。淀粉酶（G-10）单克隆抗体在蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）中表现可靠。抗淀粉酶抗体（G-10）有非结合型和多种结合型，包括辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，适用于各种实验流程。在人类中，淀粉酶以不同的单倍型存在，影响唾液和胰腺同工酶的数量；而在小鼠中，唾液腺和肝脏中不同的表达模式凸显了其生理意义。淀粉酶（G-10）单克隆抗体能够对淀粉酶同工酶进行可靠的检测，帮助研究人员揭示与淀粉酶活性相关的代谢途径、酶功能和疾病机制的复杂性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Amylase 抗体 (G-10) 参考文献:

  1. 继发于产淀粉酶浆液性卵巢囊肿的 '假性筋膜炎'。病例研究。  |  Brophy, CM., et al. 1989. J Clin Gastroenterol. 11: 703-6. PMID: 2479672
  2. 人类α-淀粉酶多基因家族由基因数量不等的单倍型组成。  |  Groot, PC., et al. 1989. Genomics. 5: 29-42. PMID: 2788608
  3. 植物α-淀粉酶抑制剂及其对饮食中多糖利用的影响。  |  Kurhajec, S. and Franc, A. 2019. Ceska Slov Farm. 68: 148-156. PMID: 31822107
  4. 肿瘤产生的淀粉酶的特征。  |  Takeuchi, T., et al. 1981. Clin Chem. 27: 556-9. PMID: 6162591
  5. 小鼠α-淀粉酶基因的组织特异性表达。  |  Schibler, U., et al. 1982. Adv Exp Med Biol. 158: 381-5. PMID: 6186131
  6. α-淀粉酶抑制剂。  |  Taylor, RH. and Barker, HM. 1983. Lancet. 1: 1228-9. PMID: 6190054
  7. 来自人类浆液性卵巢肿瘤的淀粉酶：纯化和表征。  |  Zakowski, JJ., et al. 1984. Clin Chem. 30: 62-8. PMID: 6197207
  8. 淀粉酶在胰腺疾病诊断中的应用。  |  Berk, JE. 1978. Ann Intern Med. 88: 838-9. PMID: 666145
  9. 瘤胃链球菌淀粉酶活性编码基因的调控和克隆。  |  Cotta, MA. and Whitehead, TR. 1993. Appl Environ Microbiol. 59: 189-96. PMID: 7679887
  10. 人类淀粉酶基因的非凡进化史。  |  Meisler, MH. and Ting, CN. 1993. Crit Rev Oral Biol Med. 4: 503-9. PMID: 7690604
  11. 饥饿对大鼠腮腺淀粉酶分泌昼夜变化的影响  |  Ishikawa, Y., et al. 1993. Res Exp Med (Berl). 193: 255-62. PMID: 7694345
  12. 曲霉菌株的淀粉酶活性--有机酸的生产者。  |  Tsekova, K., et al. 1993. Acta Microbiol Bulg. 30: 47-50. PMID: 8285132

订购信息

产品名称产品编号规格价格数量收藏夹

Amylase 抗体 (G-10)

sc-46657
200 µg/ml
RMB2377.00

Amylase (G-10): m-IgG Fc BP-HRP 套装

sc-528491
200 µg Ab; 10 µg BP
RMB2662.00

Amylase (G-10): m-IgGκ BP-HRP 套装

sc-520882
200 µg Ab, 40 µg BP
RMB2662.00

Amylase (G-10): m-IgG2a BP-HRP 套装

sc-547106
200 µg Ab; 10 µg BP
RMB2662.00

Amylase 抗体 (G-10) AC

sc-46657 AC
500 µg/ml, 25% agarose
RMB3129.00

Amylase 抗体 (G-10) HRP

sc-46657 HRP
200 µg/ml
RMB2377.00

Amylase 抗体 (G-10) FITC

sc-46657 FITC
200 µg/ml
RMB2482.00

Amylase 抗体 (G-10) PE

sc-46657 PE
200 µg/ml
RMB2580.00

Amylase 抗体 (G-10) Alexa Fluor® 488

sc-46657 AF488
200 µg/ml
RMB2685.00

Amylase 抗体 (G-10) Alexa Fluor® 546

sc-46657 AF546
200 µg/ml
RMB2685.00

Amylase 抗体 (G-10) Alexa Fluor® 594

sc-46657 AF594
200 µg/ml
RMB2685.00

Amylase 抗体 (G-10) Alexa Fluor® 647

sc-46657 AF647
200 µg/ml
RMB2685.00

Amylase 抗体 (G-10) Alexa Fluor® 680

sc-46657 AF680
200 µg/ml
RMB2685.00

Amylase 抗体 (G-10) Alexa Fluor® 790

sc-46657 AF790
200 µg/ml
RMB2685.00

Do you offer trial sizes of your antibodies? I want to see if your amylase antibody (sc-46657) works in Xenopus.

Asked by: Jorel
Thank you for your question. Please contact our technical service team directly to arrange for a free sample of this antibody.
Answered by: Tech Support Europe
Date published: 2022-10-25

What secondary is best to use with Amylase Antibody (G-10): sc-46657?

Asked by: jenni
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with Amylase (G-10): sc-46657, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 2
Date published: 2017-01-09
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Rated 5 out of 5 by from IHC Assay Review for Salivary Amylase mABApplication: Immunohistochemistry. Protocol: Standard protocol for saliva smear staining. Dilutions tested: 1:10, 1:30, 1:90, 1:270. Results: Over stained at dilution factor of 270. Will test dilution factors of 500, 1000, 1500, and 2000.
Date published: 2017-08-31
Rated 5 out of 5 by from Positive signalExcellent results by Immunoperoxidase, on human pancreas tissue 1:200, strong staining.
Date published: 2017-08-16
Rated 5 out of 5 by from good band no backgroundThis antibody gave us good result of WB, nice performance ,we can see target band.
Date published: 2017-06-11
Rated 5 out of 5 by from obtained strong bandsGood data by WB obtained using rat hippocampus and tissue from the pre-frontal lobe of the brain,nice strong band.
Date published: 2017-02-25
Rated 5 out of 5 by from Excellent resultsExcellent results by WB on mouse skeletal muscle 1:200.
Date published: 2017-02-25
Rated 5 out of 5 by from Great amylase detectionA great antibody that detects several amylase isoforms in standard lab assays. It is reactive with mouse, rat and human and will provide clear signal. It has been cited over 30 times!
Date published: 2017-01-16
Rated 5 out of 5 by from clean bandsWorked well for western blot analysis; stong, clean bands in rat and mouse pancreas tissue extracts.
Date published: 2016-12-17
Rated 5 out of 5 by from Performed ICC using human embryonic stem cell 2Performed ICC using human embryonic stem cell 2, 4, 8, 9. -SCBT Publication Review
Date published: 2015-06-24
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Amylase 抗体 (G-10) is rated 5.0 out of 5 by 12.
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