Date published: 2025-9-10

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Amphiphysin II 抗体 (99D): sc-13575

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说明书
  • Amphiphysin II抗体(99D)是小鼠单克隆IgG2b κ, 在11篇文献中引用,规格为200 µg/ml
  • 培养自human来源的Amphiphysin II外显子13
  • 推荐用于 mouse, rat, human 和 avian 来源的Amphiphysin II splice variants 1-10 WB, IP, IF 和 IHC(P)检测
  • 关于如何获取Amphiphysin II (99D): sc-13575免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRPm-IgGκ BP-HRP 是 Amphiphysin II 抗体 (99D) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Amphiphysin II 抗体 (99D) 组合成套装提供。 (请参阅下面的订购信息)。

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Amphiphysin II抗体(99D)是一种IgG2b κ小鼠单克隆Amphiphysin II抗体,可通过WB、IP、IF和IHC(P)检测小鼠、大鼠和人类来源的Amphiphysin II剪接变体1-10。Amphiphysin II抗体(99D)可作为非偶联的抗Amphiphysin II抗体形式。Amphiphysin是一种富含大脑的蛋白质,具有N端脂质相互作用并以二聚体的形式发挥作用。Amphiphysin包含一个膜弯曲BAR结构域、一个中间Clathrin和适配器结合结构域以及一个C端SH3结构域。在大脑中,Amphiphysin I和II形成异二聚体,通过它们的SH3结构域与Clathrin相关的GTPase Dynamin结合。这种关联对神经元中的突触小泡再循环至关重要,因为它先于Dynamin与Clathrin包被坑的结合和随后的囊泡出芽。在其他组织中,Amphiphysin可能在其他膜弯曲和曲率稳定事件中发挥关键作用。哺乳动物的Amphiphysins,即Amphiphysin I和Amphiphysin II,具有相似的整体结构。一种普遍存在的Amphiphysin II剪接形式不包含Clathrin或适配器相互作用,在肌肉组织中高度表达,并参与T小管网络的形成和稳定。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Amphiphysin II 抗体 (99D) 参考文献:

  1. 桥接整合素1(BIN1):形式、功能与阿尔茨海默病。  |  Tan, MS., et al. 2013. Trends Mol Med. 19: 594-603. PMID: 23871436
  2. 生理和疾病中的双亲蛋白-2(BIN1)。  |  Prokic, I., et al. 2014. J Mol Med (Berl). 92: 453-63. PMID: 24590001
  3. 桥接整合素1(BIN1)在阿尔茨海默病中的机制作用。  |  Gao, P., et al. 2021. Cell Mol Neurobiol. 41: 1431-1440. PMID: 32719966
  4. 在认知能力正常的老年人中,桥接整合子1(BIN1)rs744373多态性与tau-PET负荷之间缺乏关联。  |  Schaeverbeke, J., et al. 2022. Alzheimers Dement (N Y). 8: e12227. PMID: 35229019
  5. BIN1 是小胶质细胞促炎和神经变性相关激活的关键调节因子。  |  Sudwarts, A., et al. 2022. Mol Neurodegener. 17: 33. PMID: 35526014
  6. 桥接整合子 1 (BIN1, rs6733839) 和性别是血管健康预测记忆衰老轨迹的调节因子。  |  Heal, M., et al. 2022. J Alzheimers Dis. 89: 265-281. PMID: 35871342
  7. 桥接整合酶1片段通过增强小鼠的溶酶体介导的内吞作用,加速了tau聚合和传播。  |  Zhang, X., et al. 2024. PLoS Biol. 22: e3002470. PMID: 38206965
  8. 心脏和骨骼肌中的双亲蛋白-2(BIN1)的功能和缺陷。  |  Giraud, Q. and Laporte, J. 2024. Trends Mol Med. 30: 579-591. PMID: 38514365

订购信息

产品名称产品编号规格价格数量收藏夹

Amphiphysin II 抗体 (99D)

sc-13575
200 µg/ml
RMB2377.00

Amphiphysin II (99D): m-IgGκ BP-HRP 套装

sc-520630
200 µg Ab, 40 µg BP
RMB2662.00

Amphiphysin II (99D): m-IgG2b BP-HRP 套装

sc-548843
200 µg Ab; 10 µg BP
RMB2662.00

Can this Amphiphysin II (99D): sc-13575 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. We recommend following our recommended protocol, provided below, https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining Alternatively, consider using our alternative antibody, Amphiphysin II (2F11): sc-23918, which we do offer directly conjugated to HRP and a variety of fluorochromes, if you wish to omit using any secondary detection reagent and potential non-specific binding.
Answered by: Technical Support
Date published: 2017-01-30
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Rated 5 out of 5 by from Produced publishable WB data on humanStrong Amphiphysin II antibody with references in primary literature for WB in LNCaP cells
Date published: 2017-01-12
Rated 5 out of 5 by from Published western blot data using humanPublished western blot data using human, rat fibroblasts and mouse myoblasts. -SCBT Publication Review
Date published: 2015-05-04
Rated 5 out of 5 by from Clean results in western blot using truncatedClean results in western blot using truncated human Amphiphysin II transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2015-02-25
Rated 5 out of 5 by from WB: robust band in Sol8 extractWB: robust band in Sol8 extract. -SCBT QC
Date published: 2015-01-15
Rated 5 out of 5 by from Beautiful immunoperoxidase nuclear and cytoplasmicBeautiful immunoperoxidase nuclear and cytoplasmic staining in formalin-fixed, paraffin-embedded human lung tumor. -SCBT QC
Date published: 2013-04-13
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Amphiphysin II 抗体 (99D) is rated 5.0 out of 5 by 5.
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