Date published: 2025-9-8

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Ameloblastin 抗体 (H-2): sc-271012

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说明书
  • Ameloblastin 抗体 H-2 是小鼠单克隆 IgG1 κ,Ameloblastin抗体, 在4篇文献中引用,规格为200 µg/ml
  • 针对氨基酸148-447的抗原,在Ameloblastin的C-terminus处映射,human来源
  • Ameloblastin 抗体 (H-2) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 Ameloblastin
  • 抗Ameloblastin抗体(H-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ameloblastin (H-2): sc-271012免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 Ameloblastin 抗体 (H-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Ameloblastin 抗体 (H-2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Ameloblastin抗体(H-2)是一种IgG1 κ小鼠单克隆Ameloblastin抗体(也称为Ameloblastin抗体),可通过WB、IP、IF、IHC(P)和ELISA检测人类来源的Ameloblastin蛋白。Ameloblastin抗体(H-2)既有非偶联抗Ameloblastin抗体形式,也有多种偶联形式的抗Ameloblastin抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。牙釉质是一种高度矿化的组织,其大部分体积由大而有组织的羟基磷灰石晶体占据。人们认为,这种结构是通过许多有机基质分子的相互作用来控制的,包括釉原蛋白、成釉蛋白、釉蛋白、簇绒蛋白和几种其他酶。所有这些分泌蛋白都参与发育中牙釉质的矿化和牙釉质基质形成。成釉蛋白(AMBN)是一种定位于细胞外基质的成釉细胞特异性蛋白。在杆状-杆间牙釉质的鞘空间和分泌性成釉细胞的Tomes过程中检测到。编码成釉蛋白的基因AMBN的缺陷可见于成釉细胞瘤患者。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ameloblastin 抗体 (H-2) 参考文献:

  1. 人类釉母细胞蛋白的克隆, 表征和免疫定位。  |  MacDougall, M., et al. 2000. Eur J Oral Sci. 108: 303-10. PMID: 10946765
  2. 克隆和鉴定人类釉母细胞素基因。  |  Toyosawa, S., et al. 2000. Gene. 256: 1-11. PMID: 11054529
  3. 人类成釉细胞素基因:成釉不全症患者的基因组组织和突变分析。  |  Mårdh, CK., et al. 2001. Eur J Oral Sci. 109: 8-13. PMID: 11330937
  4. 小鼠出生后臼齿发育过程中釉质母细胞蛋白和釉质原蛋白的表达。  |  Torres-Quintana, MA., et al. 2005. J Oral Sci. 47: 27-34. PMID: 15881226
  5. 釉质基质蛋白的相互作用  |  Wang, H., et al. 2005. J Bone Miner Res. 20: 1032-40. PMID: 15883644
  6. 鳄鱼釉质形成过程中釉质母细胞蛋白的表达。  |  Shintani, S., et al. 2006. J Exp Zool B Mol Dev Evol. 306: 126-33. PMID: 16217799

订购信息

产品名称产品编号规格价格数量收藏夹

Ameloblastin 抗体 (H-2)

sc-271012
200 µg/ml
RMB2377.00

Ameloblastin (H-2): m-IgG Fc BP-HRP 套装

sc-527202
200 µg Ab; 10 µg BP
RMB2662.00

Ameloblastin (H-2): m-IgG1 BP-HRP 套装

sc-532575
200 µg Ab; 20 µg BP
RMB2662.00

Ameloblastin 抗体 (H-2) AC

sc-271012 AC
500 µg/ml, 25% agarose
RMB3129.00

Ameloblastin 抗体 (H-2) HRP

sc-271012 HRP
200 µg/ml
RMB2377.00

Ameloblastin 抗体 (H-2) FITC

sc-271012 FITC
200 µg/ml
RMB2482.00

Ameloblastin 抗体 (H-2) PE

sc-271012 PE
200 µg/ml
RMB2580.00

Ameloblastin 抗体 (H-2) Alexa Fluor® 488

sc-271012 AF488
200 µg/ml
RMB2685.00

Ameloblastin 抗体 (H-2) Alexa Fluor® 546

sc-271012 AF546
200 µg/ml
RMB2685.00

Ameloblastin 抗体 (H-2) Alexa Fluor® 594

sc-271012 AF594
200 µg/ml
RMB2685.00

Ameloblastin 抗体 (H-2) Alexa Fluor® 647

sc-271012 AF647
200 µg/ml
RMB2685.00

Ameloblastin 抗体 (H-2) Alexa Fluor® 680

sc-271012 AF680
200 µg/ml
RMB2685.00

Ameloblastin 抗体 (H-2) Alexa Fluor® 790

sc-271012 AF790
200 µg/ml
RMB2685.00

Can Ameloblastin (H-2): sc-271012 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, Ameloblastin (H-2): sc-271012 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 3 out of 5 by from Works for Westerns not for IHC in mouse tissueThis antibody worked for recombinant murine and native porcine Ambn Western blots but did not work in IHC staining of mouse sections. Previous M300 ameloblastin antibody (rabbit polyclonal) used to work very well (1:500 dilution for IHC) and N18 ameloblastin ab (goat polyclonal) worked at higher concentrations (1:100). I have tried both concentrations for H2 and it did not work.
Date published: 2018-11-12
Rated 1 out of 5 by from Doesn't work in rat and mouse ameloblastSC's previous polyclonal AMBN antibody worked great for me, however this new mouse mono Ab doesn't seem to be equivalent. It doesn't work in my immunofluorescent staining of rat and mouse ameloblasts (paraffin embeding and enzyme/citrate heat retrieval).
Date published: 2017-07-05
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-02-07
Rated 5 out of 5 by from Nice Western Blot data of human recombinantNice Western Blot data of human recombinant Ameloblastin fusion protein. -SCBT QC
Date published: 2014-08-04
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Ameloblastin 抗体 (H-2) is rated 3.5 out of 5 by 4.
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