Date published: 2025-9-6
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alpha Tubulin Antibody (TU-02) - Western Blotting - Image 396257
α 管蛋白抗体 (TU-02) Alexa Fluor® 680: sc-8035 AF680. NIH/3T3 (A) 和 PC-12 (B) 全细胞裂解液中 α 管蛋白表达的直接近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 790 检测 Cruz Marker™ 分子量标准: sc-516731.
α 管蛋白抗体 (TU-02) Alexa Fluor® 680: sc-8035 AF680. NIH/3T3 (A) 和 PC-12 (B) 全细胞裂解液中 α 管蛋白表达的直接近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker™ MW Tag-Alexa Fluor® 790 检测 Cruz Marker™ 分子量标准: sc-516731.

alpha Tubulin 抗体 (TU-02): sc-8035

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寫評論提問

说明书
  • alpha Tubulin抗体(TU-02)是小鼠单克隆IgM κ, 在1,490篇文献中引用，规格为200 µg/ml
  • 免疫1-451氨基酸，对应于porcine来源α Tubulin全长
  • 抗-alpha Tubulin 抗体 (TU-02) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA，检测mouse, rat, human 和porcine 来源的 α Tubulin
  • 抗alpha Tubulin抗体（TU-02）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供琼脂糖(sc-8035 AC)偶联抗体，规格为500 µg/ml, 25% agarose，可用于IP实验；以及藻红蛋白(sc-8035 PE)偶联抗体，规格为200 µg/ml，用于IF、IHC(P)和FCM实验
  • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
  • 关于如何获取alpha Tubulin (TU-02): sc-8035的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 alpha Tubulin 抗体 (TU-02) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与alpha Tubulin 抗体 (TU-02) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Tubulin 抗体 包括 α 1 Tubulin, α 3 Tubulin, α 4 Tubulin, α Tubulin, β Tubulin, β 2 Tubulin, β 3 Tubulin, δ Tubulin, ε Tubulin和γ Tubulin

α Tubulin 抗体（TU-02）是一种小鼠单克隆IgM kappa轻链抗体，针对猪源全长α Tubulin蛋白（氨基酸1-451）产生。α Tubulin 单克隆抗体（TU-02）可检测包括小鼠、大鼠、人类和猪在内的多种物种的α Tubulin，可用于 技术，如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）、流式细胞术（FCM）和酶联免疫吸附测定（ELISA）。α微管蛋白与β微管蛋白结合形成异二聚体，并组装成微管，微管是细胞形状、细胞内运输和有丝分裂所必需的结构。这些微管在细胞分裂过程中尤为重要，它们形成有丝分裂纺锤体，引导染色体分离。α-微管蛋白主要位于细胞质中，构成微管的组成部分，并延伸至整个细胞。α-微管蛋白在维持细胞完整性和支持微管动态方面发挥着重要作用，这使得抗-α-微管蛋白抗体（TU-02）成为研究细胞骨架和相关细胞过程的强大工具。α-微管蛋白 蛋白（TU-02）抗体有非结合型和结合型两种形式，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和Alexa Fluor®结合物，可满足各种实验需求。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

alpha Tubulin 抗体 (TU-02) 参考文献:

  1. 硬草中α-微管蛋白转录本的多样性。  |  Chen, J., et al. 2021. Pest Manag Sci. 77: 970-977. PMID: 32991064
  2. α-微管蛋白的SUMO化是一种调节微管动力学的新修饰。  |  Feng, W., et al. 2021. J Mol Cell Biol. 13: 91-103. PMID: 33394042
  3. 血管抑制素-1在肾小球足细胞中具有α-微管蛋白脱酪氨酸活性。  |  Mifune, T., et al. 2022. Biochem Biophys Res Commun. 599: 93-99. PMID: 35180473
  4. 射血分数正常的心力衰竭（HFpEF）中的微管蛋白表达和修饰。  |  Schulz, L., et al. 2022. Sci Rep. 12: 15734. PMID: 36131110
  5. α-微管蛋白的重新定位参与草酸钙诱导的肾上皮细胞紧密连接破坏。  |  Hadpech, S., et al. 2022. Chem Biol Interact. 368: 110236. PMID: 36349591
  6. 微管蛋白同型调节维持α-微管蛋白对β-微管蛋白的不对称需求。  |  Wethekam, LC. and Moore, JK. 2023. J Cell Biol. 222: PMID: 36719400
  7. 人类α-微管蛋白同种型对微管蛋白酪氨酸化和微管稳定性的整合调控。  |  Fu, G., et al. 2023. Cell Rep. 42: 112653. PMID: 37379209
  8. 乙酰化α-微管蛋白和α-突触核蛋白：生理相互作用及其对α-突触核蛋白寡聚化的贡献。  |  Calogero, AM., et al. 2023. Int J Mol Sci. 24: PMID: 37569662
  9. 抑制α-微管蛋白乙酰化可增强依立布林在肝癌中的治疗效果。  |  Zhong, Y., et al. 2023. Am J Cancer Res. 13: 5698-5718. PMID: 38058833
  10. HDAC6催化的α-微管蛋白乳酰化对细胞骨架功能的代谢调节。  |  Sun, S., et al. 2024. Nat Commun. 15: 8377. PMID: 39333081

订购信息

产品名称产品编号规格价格数量收藏夹

alpha Tubulin 抗体 (TU-02)

sc-8035
200 µg/ml
RMB2377.00

alpha Tubulin (TU-02): m-IgGκ BP-HRP 套装

sc-550728
200 µg Ab; 40 µg BP
RMB2662.00

alpha Tubulin 抗体 (TU-02) AC

sc-8035 AC
500 µg/ml, 25% agarose
RMB3129.00

alpha Tubulin 抗体 (TU-02) HRP

sc-8035 HRP
200 µg/ml
RMB2377.00

alpha Tubulin 抗体 (TU-02) FITC

sc-8035 FITC
200 µg/ml
RMB2482.00

alpha Tubulin 抗体 (TU-02) PE

sc-8035 PE
200 µg/ml
RMB2580.00

alpha Tubulin 抗体 (TU-02) Alexa Fluor® 488

sc-8035 AF488
200 µg/ml
RMB2685.00

alpha Tubulin 抗体 (TU-02) Alexa Fluor® 546

sc-8035 AF546
200 µg/ml
RMB2685.00

alpha Tubulin 抗体 (TU-02) Alexa Fluor® 594

sc-8035 AF594
200 µg/ml
RMB2685.00

alpha Tubulin 抗体 (TU-02) Alexa Fluor® 647

sc-8035 AF647
200 µg/ml
RMB2685.00

alpha Tubulin 抗体 (TU-02) Alexa Fluor® 680

sc-8035 AF680
200 µg/ml
RMB2685.00

alpha Tubulin 抗体 (TU-02) Alexa Fluor® 790

sc-8035 AF790
200 µg/ml
RMB2685.00

alpha Tubulin 抗体 (TU-02) TRITC

sc-8035 TRITC
200 µg/ml
RMB2407.00

alpha Tubulin 抗体 (TU-02) Alexa Fluor® 405

sc-8035 AF405
200 µg/ml
RMB2685.00

i have used SC-8035 primary antibody with sc-2064 secondary antibody. it seems sc-2064 is no more available. what secondary i should use for western blotting.

Asked by: Nandita
Thank you for your question. We recommend using an anti-mouse IgM secondary. Santa Cruz Biotechnology do not offer IgM secondaries .
Answered by: BlakeJ
Date published: 2022-11-14

LOT# D0921 did not work for WB against human and mouse whole cell lysates (C2C12, 3T3-L1, and HEK293 cells). I think it may have a problem with this LOT# product. Would you check whether is it okay? 

Asked by: Stiger22
Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Service SH
Date published: 2022-07-04

Is this antibody recommended for using as a loading control for western blotting of both Mouse and human samples?

Asked by: Islam
Yes, this antibody can be used for this purpose.
Answered by: Dallas Tech Service
Date published: 2021-06-18

Hi, I have purchased this antibody a few weeks ago. Initially, the band took so long to appear at 55kDa. The second time I tried, another band appear at 45kDa which is more intense. The band at 55kDa took more than 3 hours to develop

Asked by: Siti Khadijah
Thank you for your message. Please get in touch with our technical service team directly so we can troubleshoot your experiment.
Answered by: Tech Support Europe
Date published: 2020-08-05

Liebes Santa Cruz Team, besteht die Möglichkeit ein Testsample oder kleineres Muster von α Tubulin Antikörper (TU-02): sc-8035 mit FITC oder ähnlichem konjugiert zu erhalten? In welcher Verdünnung wird der AK für die IF eingesetzt? Vielen dank

Asked by: AKKlein
Vielen Dank für Ihre Anfrage. Wir bieten nur unkonjugierte Primärantikörper als kostenlose Samples an. Sie können auch unsere empfohlenen sekundären Bindeproteine als Samples erhalten, wie z.B. m-IgGκ BP-FITC: sc-516140 oder m-IgGκ BP-CFL 488: sc-516176. Kontaktieren Sie gerne unseren technischen Service um die Lieferung entsprechender Samples zu arrangieren.
Answered by: Tech Support Europe
Date published: 2019-06-14

Has this antibody been used on mouse brain sections?

Asked by: Alex3
Thank you for your question. This antibody is fine for mouse tissue sections, so it should be fine for your mouse brain samples. If you have any further questions, please reach out to Technical support at [email protected], or via phone at 800-457-3801, ext 2 or via chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-12-04

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Cweed
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. SC-8035 is currently available conjugated to FITC, AF488, AF647.
Answered by: TechService7
Date published: 2016-12-14

How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Cweed
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. SC-8035 is currently available conjugated to AF488, AF647, and FITC.
Answered by: TechService7
Date published: 2016-12-14
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Rated 4 out of 5 by from Buon anticorpoHo testato l'ab in immunofluorescenza su neurone e ho ottenuto un ottimo risultato con diluizione 1:250.
Date published: 2024-07-22
Rated 5 out of 5 by from Great for WBThe antibody is good for western blot. The band is single and clear.
Date published: 2024-03-19
Rated 5 out of 5 by from Muy buen reconocimiento de la proteínaGood antibody for tubulin detection in WB. It gives a strong signal.
Date published: 2023-10-03
Rated 5 out of 5 by from sc-8035We use this antibody in western blot, and it works well.
Date published: 2023-07-07
Rated 4 out of 5 by from Sharp and single bandI am so happy to use this antibody for paper publication.
Date published: 2023-03-14
Rated 5 out of 5 by from Excellent antibody!Good for both immunoblotting and immunofluorescence of human proteins.
Date published: 2020-10-09
Rated 5 out of 5 by from 很好用帮客户买的，客户评价很好用，条带清晰。没有杂带。会继续回购的。对课题很有帮助。希望多一点折扣。经费在燃烧
Date published: 2020-09-18
Rated 5 out of 5 by from Good antibody for western blottinga-Tubulin Antibody (TU-02) is good quality for western blotting (dilute factor: 1:5000)
Date published: 2020-05-25
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alpha Tubulin 抗体 (TU-02) is rated 4.7 out of 5 by 79.
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