Date published: 2025-9-6
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alpha Tubulin Antibody (DM1A) - Western Blotting - Image 392264
α 管蛋白抗体 (DM1A) Alexa Fluor® 488: sc-32293 AF488. PC-12 (A), Hep G2 (B), Jurkat (C), A549 (D) 和 K-562 (E) 全细胞裂解液中 α 管蛋白表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 647 检测 Cruz Marker™ 分子量标准: sc-516791.
α 管蛋白抗体 (DM1A) Alexa Fluor® 488: sc-32293 AF488. PC-12 (A), Hep G2 (B), Jurkat (C), A549 (D) 和 K-562 (E) 全细胞裂解液中 α 管蛋白表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 647 检测 Cruz Marker™ 分子量标准: sc-516791.

alpha Tubulin 抗体 (DM1A): sc-32293

4.8(26)
寫評論提問

说明书
  • alpha Tubulin抗体(DM1A)是小鼠单克隆IgG1 κ, 在672篇文献中引用，规格为200 µg/ml
  • 针对天然小鸡脑微管
  • 抗-alpha Tubulin 抗体 (DM1A) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat, human 和avian 来源的 α Tubulin
  • 抗alpha Tubulin抗体（DM1A）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取alpha Tubulin (DM1A): sc-32293的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 alpha Tubulin 抗体 (DM1A) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与alpha Tubulin 抗体 (DM1A) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Tubulin抗体，包括α 1 Tubulin, α 3 Tubulin, α 4 Tubulin, α Tubulin, β Tubulin, β 2 Tubulin, β 3 Tubulin, δ Tubulin, ε Tubulin和γ Tubulin

α Tubulin 抗体（DM1A）是一种小鼠单克隆 IgG1 kappa 轻链抗体，针对天然小鸡脑微管产生。抗-α Tubulin 抗体（DM1A）专门针对多种物种的 α Tubulin，包括小鼠、大鼠、人类和鸟类，适用于多种应用，如蛋白质印迹（WB）、免疫 免疫沉淀（IP）、免疫荧光（IF）以及石蜡包埋切片免疫组织化学（IHCP）等多种应用。α-微管蛋白是细胞骨架的关键结构成分，与β-微管蛋白形成异二聚体，并组装成微管，微管是维持细胞形状、实现细胞内运输和促进细胞分裂的关键动态丝。α-微管蛋白的结构对于其在微管聚合中的作用至关重要，因为α-微管蛋白为微管提供了快速组装和拆卸所需的稳定性和灵活性，以响应细胞信号。α Tubulin与β Tubulin等其他Tubulin同源物一起，在细胞分裂过程中对有丝分裂纺锤体的形成至关重要，可确保染色体准确分离。α Tubulin位于细胞质中，参与微管的形成，微管延伸至整个细胞，为细胞内运输和细胞器定位提供支架。α Tubulin单克隆抗体（DM1A）有非结合 ，包括辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）、琼脂糖和Alexa Fluor®偶联物，为各种实验设计提供了灵活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

alpha Tubulin 抗体 (DM1A)

sc-32293
200 µg/ml
RMB2377.00

alpha Tubulin (DM1A): m-IgG Fc BP-HRP 套装

sc-526675
200 µg Ab; 10 µg BP
RMB2662.00

alpha Tubulin (DM1A): m-IgG1 BP-HRP 套装

sc-532048
200 µg Ab; 20 µg BP
RMB2662.00

alpha Tubulin 抗体 (DM1A) AC

sc-32293 AC
500 µg/ml, 25% agarose
RMB3129.00

alpha Tubulin 抗体 (DM1A) HRP

sc-32293 HRP
200 µg/ml
RMB2377.00

alpha Tubulin 抗体 (DM1A) FITC

sc-32293 FITC
200 µg/ml
RMB2482.00

alpha Tubulin 抗体 (DM1A) PE

sc-32293 PE
200 µg/ml
RMB2580.00

alpha Tubulin 抗体 (DM1A) Alexa Fluor® 488

sc-32293 AF488
200 µg/ml
RMB2685.00

alpha Tubulin 抗体 (DM1A) Alexa Fluor® 546

sc-32293 AF546
200 µg/ml
RMB2685.00

alpha Tubulin 抗体 (DM1A) Alexa Fluor® 594

sc-32293 AF594
200 µg/ml
RMB2685.00

alpha Tubulin 抗体 (DM1A) Alexa Fluor® 647

sc-32293 AF647
200 µg/ml
RMB2685.00

alpha Tubulin 抗体 (DM1A) Alexa Fluor® 680

sc-32293 AF680
200 µg/ml
RMB2685.00

alpha Tubulin 抗体 (DM1A) Alexa Fluor® 790

sc-32293 AF790
200 µg/ml
RMB2685.00

为什么我们跑出来很多杂带

Asked by: 余霞YYYYY
感谢您的提问。您可以拨打电话：021-60936350，发送邮件到[email protected]，或者我们官网上的在线聊天，联系我们的亚洲技术支持团队，了解更多信息。 Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Service SH
Date published: 2025-08-08

I saw that people are using 1:2000 dilution for Western blot .Just wondering about the incubation time (2h at room temp or overnight at 4 degree)??

Asked by: sasw1
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-03-02

Hi, I cannot find on this page an amount of the α Tubulin Antibody (DM1A) sc-32293? It says 200ug/ml, but does not say the volume. Is this antibody genetically identical to T6199 SIGMA? Is it available in -20C buffer?

Asked by: V_M_
Thank you for your question. This antibody is provided as 200 ug IgG in 1 ml. Yes, this clone DM1A is commercially available from other suppliers. Please contact our Special Orders Department to inquire about alternate formulations suitable for -20C storage.
Answered by: Technical Support
Date published: 2019-05-25

How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jerojero
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody.
Answered by: Tech Service 3
Date published: 2016-12-16

I need to use an antibody to detect the protein in samples of avian origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 3
Date published: 2016-12-16
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Rated 4 out of 5 by from SCBT Alpha TubulinThe Tubulin antibody used in 1:1000 dilution worked well
Date published: 2025-06-25
Rated 5 out of 5 by from Excellent!I detected strong and clear band in WB of mouse neural cells.
Date published: 2024-03-29
Rated 5 out of 5 by from Muy buen reconocimiento de la proteínaBuen reconocimiento en WB, sin ruido de fondo y banda muy clara
Date published: 2023-10-03
Rated 5 out of 5 by from Great!Great product! Works great! Better to get from here than anywhere else.
Date published: 2022-07-22
Rated 5 out of 5 by from alpha-tubulin: IF staining of FFPE sectionsGreat for immunofluorescence staining of FFPE sections. Stains cilia of airway epithelial cells. Use at 1:250. Works by HIER antigen retrieval in EDTA pH8.0, 15 mins.
Date published: 2021-07-23
Rated 5 out of 5 by from Great antibodyThis antibody works fantastically on PFA fixed fly larval tissues. Also works beautifully on western!
Date published: 2021-04-23
Rated 5 out of 5 by from Fantastic antibody for western blotSame as that from Sigma or Invitrogen, diluted 1:2000 and can be reused multiple times
Date published: 2021-03-31
Rated 5 out of 5 by from Excellent for WB and immunofluorescenceIn our hands, we need to fix with PFA 4% and permeabilize with saponin, not Triton, the latter extracts the epitope. But the antibody is clean and very good.
Date published: 2020-11-20
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alpha Tubulin 抗体 (DM1A) is rated 4.8 out of 5 by 27.
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