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α/β/γPAK Antibody (D-8) - Western Blotting - Image 381137 — α/β/γPAK (D-8): sc-166174. HeLa (A), SH-SY5Y (B) 和 EOC 20 (C) 全细胞裂解液中 α/β/γPAK 表达的 Western 印迹分析.

α/β/γPAK (D-8): sc-166174. HeLa (A), SH-SY5Y (B) 和 EOC 20 (C) 全细胞裂解液中 α/β/γPAK 表达的 Western 印迹分析.

α/β/γPAK 抗体 (D-8): sc-166174

Name: &alpha;/&beta;/&gamma;PAK抗体 (D-8)
Brand: SCBIO
SKU: sc-166174
Price: 2377.0 CNY
Availability: InStock

3.9(8)

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说明书

alpha / beta / gamma PAK 抗体 D-8 是小鼠单克隆 IgG₁ κ，α/β/γPAK抗体, 在6篇文献中引用，规格为200 µg/ml
针对氨基酸263-345的图谱，在βPAK的内部区域，mouse起源
alpha / beta / gamma PAK 抗体 (D-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 αPAK, βPAK and γPAK
抗alpha / beta / gamma PAK抗体（D-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取α/β/γPAK (D-8): sc-166174的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG₁ BP-HRP 是 α/β/γPAK 抗体 (D-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与α/β/γPAK 抗体 (D-8) 组合成套装提供。 (请参阅下面的订购信息)。

查看产品引用文献(6)

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α/β/γPAK 抗体 (D-8) 参考文献:

p21 激活的蛋白激酶 gamma-PAK 被电离辐射和其他 DNA 损伤剂激活。与 alpha-PAK 有相似之处，也有不同之处。 | Roig, J. and Traugh, JA. 1999. J Biol Chem. 274: 31119-22. PMID: 10531298
在碱性成纤维细胞生长因子诱导的神经元生长过程中，betaPak-interacting交换因子介导的Rac1活化需要smgGDS鸟嘌呤核苷酸交换因子。 | Shin, EY., et al. 2006. J Biol Chem. 281: 35954-64. PMID: 16954223
人胎盘 GTP 结合蛋白 Gp（G25K）基因的分子克隆：确定该 GTP 结合蛋白为酵母细胞分裂周期蛋白 CDC42 的人类同源物。 | Shinjo, K., et al. 1990. Proc Natl Acad Sci U S A. 87: 9853-7. PMID: 2124704
rac，一种与 ras 相关的新型蛋白质家族，是肉毒毒素的底物。 | Didsbury, J., et al. 1989. J Biol Chem. 264: 16378-82. PMID: 2674130
小 GTP 结合蛋白 Rac1 和 Cdc42 可调节 JNK/SAPK 信号通路的活性。 | Coso, OA., et al. 1995. Cell. 81: 1137-46. PMID: 7600581
一种由 rac1/CDC42Hs 依赖性自身磷酸化激活的新型丝氨酸激酶与 PAK65 和 STE20 有关。 | Martin, GA., et al. 1995. EMBO J. 14: 1970-8. PMID: 7744004
Raf-1 和 MEKK 对 ERK 和 JNK 丝裂原活化蛋白激酶的不同激活作用。 | Minden, A., et al. 1994. Science. 266: 1719-23. PMID: 7992057
MEKK1 磷酸化活化剂 SEK1 激活应激活化蛋白激酶。 | Yan, M., et al. Nature. 372: 798-800. PMID: 7997270
一种由 Cdc42 和 Rac1 激活的脑丝氨酸/苏氨酸蛋白激酶。 | Manser, E., et al. 1994. Nature. 367: 40-6. PMID: 8107774
调节 Ras 及其近亲的蛋白质。 | Boguski, MS. and McCormick, F. 1993. Nature. 366: 643-54. PMID: 8259209
来自胎盘和淋巴细胞的人类 cdc42 依赖性 S6/H4 激酶/gammaPAK 的肌球蛋白磷酸化。 | Ramos, E., et al. 1997. Recept Signal Transduct. 7: 99-110. PMID: 9392438
αPAK中一个保守的负调控区：抑制PAK激酶可揭示其在Cdc42和Rac1下游的形态学作用。 | Zhao, ZS., et al. 1998. Mol Cell Biol. 18: 2153-63. PMID: 9528787

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
α/β/γPAK 抗体 (D-8)	sc-166174	200 µg/ml	RMB2377.00
α/β/γPAK (D-8): m-IgG₁ BP-HRP 套装	sc-531676	200 µg Ab; 20 µg BP	RMB2662.00
α/β/γPAK 抗体 ( (D-8) AC	sc-166174 AC	500 µg/ml, 25% agarose	RMB3129.00
α/β/γPAK 抗体 ( (D-8) HRP	sc-166174 HRP	200 µg/ml	RMB2377.00
α/β/γPAK 抗体 ( (D-8) FITC	sc-166174 FITC	200 µg/ml	RMB2482.00
α/β/γPAK 抗体 ( (D-8) PE	sc-166174 PE	200 µg/ml	RMB2580.00
α/β/γPAK 抗体 ( (D-8) Alexa Fluor^® 488	sc-166174 AF488	200 µg/ml	RMB2685.00
α/β/γPAK 抗体 (D-8) Alexa Fluor^® 546	sc-166174 AF546	200 µg/ml	RMB2685.00
α/β/γPAK 抗体 ( (D-8) Alexa Fluor^® 594	sc-166174 AF594	200 µg/ml	RMB2685.00
α/β/γPAK 抗体 ( (D-8) Alexa Fluor^® 647	sc-166174 AF647	200 µg/ml	RMB2685.00
α/β/γPAK 抗体 ( (D-8) Alexa Fluor^® 680	sc-166174 AF680	200 µg/ml	RMB2685.00
α/β/γPAK 抗体 ( (D-8) Alexa Fluor^® 790	sc-166174 AF790	200 µg/ml	RMB2685.00

What is the recommended fixation method for immunofluorescence staining with α/β/γPAK ( (D-8): sc-166174 monoclonal antibody?

Asked by: Dr Ninau Qelp

Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining

Answered by: Technical Support

Date published: 2017-02-24

Rated 4 out of 5 by StanK from good in WBThe background is a little too high and have to use at low dilution. Otherwise, good antibody for WB.

Date published: 2018-04-13

Rated 5 out of 5 by 小新贝贝 from Work well!1:200 diluted for wb of rat heart protein,the band is strong and special

Date published: 2018-04-10

Rated 1 out of 5 by RGM_Lab from No signal detectedUsed a 1:300 dilution for WB with 1- SUM159, 2- Caco-2, and 3- 293-T cell lysates. After a long exposure, no signal detected.

Date published: 2018-01-30

Rated 1 out of 5 by MadhuDey from α/β/γPAK Antibody (D-8)I got a free sample. The antibody did not show any band at the right size..

Date published: 2017-10-29

Rated 5 out of 5 by Steven Pelech at Kinexus from Excellent antibody for Western blotting.This product has been independently tested and validated in-house at Kinexus Bioinformatics Corporation as a very potent and highly specific antibody for Western blotting with a mixture of mouse brain, testes and thymus tissue lysates. It very strongly detects appropriately sized 60-65 kDa target proteins in immunoblots.

Date published: 2016-10-13

Rated 5 out of 5 by FL from WB: Strong banding using NIH/3T3 and SKWB: Strong banding using NIH/3T3 and SK-N-MC whole cell lysates. -SCBT QC

Date published: 2015-06-16

Rated 5 out of 5 by SM from Worked great for IF with methanolWorked great for IF with methanol-fixed HeLa cells.

Date published: 2015-03-05

Rated 5 out of 5 by MS from Great immunoperoxidase cytoplasmic stainingGreat immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human cerebral cortex tissue. -SCBT QC

Date published: 2015-01-30

α/β/γPAK 抗体 (D-8) is rated 3.9 out of 5 by 8.

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种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	PAK3	Entrez Gene ID: 5063	Xq23	XM_005262132, XM_005262133, XM_006724654, XM_006724655, XM_011530962, XM_011530967, XM_011530968, XM_011530969, XM_011530971, XM_017029557, XM_017029558, XM_017029559, XM_017029560, XM_017029561, XM_017029562, XM_017029563, NM_001128166, NM_001128167, NM_001128168, NM_001128172, NM_001128173, NM_001324325, NM_001324326, NM_001324327, NM_001324328, NM_001324329, NM_001324330, NM_001324331, NM_001324332, NM_001324333, NM_001324334, NM_002578	UniProt ID: O75914	300558
Mouse	Pak1	Entrez Gene ID: 18479	7 E2	XM_006507434, XM_006507436, XM_006507437, XM_011241694, XM_017322028, NM_011035	UniProt ID: O88643	N/A
Mouse	Pak3	Entrez Gene ID: 18481	X F2	XM_006528747, XM_006528752, XM_006528753, XM_006528754, XM_011247790, XM_017318417, XM_017318418, XM_017318419, XM_017318420, XM_017318421, XM_017318422, XM_017318423, NM_001195046, NM_001195047, NM_001195048, NM_001195049, NM_008778	UniProt ID: Q61036	N/A
Mouse	Pak2	Entrez Gene ID: 224105	16 B2	XM_006522072, NM_177326	UniProt ID: Q8CIN4	N/A

α/β/γPAK 抗体 (D-8): sc-166174

α/β/γPAK 抗体 (D-8) 参考文献:

α/β/γPAK 抗体 (D-8) 规格

替代产品名称: P21 Activated Kinase

产品克隆名称: D-8

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁ kappa light chain

氨基酸: 263-345

抗原表位靶标: βPAK

抗原表位种属: mouse

产品反应性/检测: αPAK, βPAK and γPAK

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, IHC(P), ELISA

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 6篇各种科学出版物的引文

α/β/γPAK 抗体 (D-8)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin