Date published: 2025-9-8

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ALP 抗体 (B-10): sc-365765

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说明书
  • ALP 抗体 B-10 是小鼠单克隆 IgG2a κ,ALP抗体, 在37篇文献中引用,规格为200 µg/ml
  • 针对human起源的ALP内部区域内的氨基酸44-200进行
  • ALP 抗体 (B-10) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 ALP
  • 抗ALP抗体(B-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ALP (B-10): sc-365765免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是ALP Antibody (B-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与ALP Antibody (B-10) 打包提供(请参阅下面的订购信息)。

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関連項目

碱性磷酸酶抗体(B-10)是一种IgG2a κ小鼠单克隆碱性磷酸酶抗体(也称为碱性磷酸酶抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的碱性磷酸酶蛋白。碱性磷酸酶抗体(B-10)可以是非偶联的抗碱性磷酸酶抗体形式,也可以是多种偶联形式的抗碱性磷酸酶抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。碱性磷酸酶(肌动蛋白相关LIM蛋白),也称为PDLIM3(PDZ和LIM结构域蛋白3),是一种含有1个LIM锌结合结构域和1个PDZ结构域的364个氨基酸的蛋白质,定位于细胞质和肌纤维Z线。碱性磷酸酶以三种选择性剪接的同工型存在,其中两种在骨骼肌和心脏中表现出组织特异性表达,与α-肌动蛋白-2相互作用,并通过这种相互作用在肌动蛋白丝组织中发挥作用,特异性调节肌动蛋白丝阵列与肌肉细胞的结合。编码碱性磷酸酶的基因定位于人类染色体4上与面肩肱型肌营养不良症相关的区域,表明碱性磷酸酶基因的缺陷可能与肌营养不良症的发病机制有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ALP 抗体 (B-10) 参考文献:

  1. 将肌肉特异性肌动蛋白缔合作用 LIM 蛋白 (ALP) 基因排除在 4q35 Facioscapulohumeral muscular dystrophy (FSHD) 候选基因之外。  |  Bouju, S., et al. 1999. Neuromuscul Disord. 9: 3-10. PMID: 10063829
  2. 肌动蛋白相关 LIM 蛋白中的 ZASP 样基序是与α-肌动蛋白杆相互作用并靶向肌肉 Z 线所必需的。  |  Klaavuniemi, T., et al. 2004. J Biol Chem. 279: 26402-10. PMID: 15084604
  3. 编码心肌细胞 Z 线蛋白的 PDLIM3 和 MYOZ1 基因突变在特发性扩张型心肌病中并不常见。  |  Arola, AM., et al. 2007. Mol Genet Metab. 90: 435-40. PMID: 17254821
  4. 通过基因表达谱分析确定肢腰肌营养不良症 2A 型分叶状纤维的特征。  |  Keira, Y., et al. 2007. Neurosci Res. 57: 513-21. PMID: 17258832
  5. 细胞骨架缔合作用的 PDZ-LIM 蛋白 ALP 对血清反应因子的活性起调节肌肉分化的作用。  |  Pomiès, P., et al. 2007. Mol Biol Cell. 18: 1723-33. PMID: 17332502
  6. 接受手术的髓周癌患者体内 ALP 和 LDH 的预后价值  |  Dang, C., et al. 2022. Curr Med Sci. 42: 150-158. PMID: 34669114
  7. 用于肝癌特异性治疗的 ALP 激活型化学发光 PDT 纳米平台。  |  Fan, N., et al. 2021. ACS Appl Bio Mater. 4: 1740-1748. PMID: 35014520
  8. ALP 抑制剂通过 AKT-ERK 通路抑制 hVIC 的炎症反应和成骨细胞分化  |  Zhang, C., et al. 2023. Altern Ther Health Med. 29: 58-65. PMID: 35951069
  9. BMsc-外泌体通过mTOR/TFEB轴恢复溶酶体功能,减轻ALP功能障碍,从而减轻脑缺血再灌注损伤。  |  Liu, H., et al. 2024. Exp Neurol. 376: 114726. PMID: 38403042
  10. 模拟 ALP 的环肽纳米管:骨生成和骨再生的多功能策略  |  Chawla, V., et al. 2025. Biomacromolecules.. PMID: 39952236
  11. 通过高密度 cDNA 阵列的定量杂交揭示人类肌肉中优先表达的新基因转录本。  |  Piétu, G., et al. 1996. Genome Res. 6: 492-503. PMID: 8828038
  12. 肌动蛋白缔合作用的 LIM 蛋白:鉴定 PDZ 与谱蛋白样重复图案之间的结构域相互作用。  |  Xia, H., et al. 1997. J Cell Biol. 139: 507-15. PMID: 9334352

订购信息

产品名称产品编号规格价格数量收藏夹

ALP 抗体 (B-10)

sc-365765
200 µg/ml
RMB2377.00

ALP (B-10): m-IgGκ BP-HRP 套装

sc-522356
200 µg Ab, 40 µg BP
RMB2662.00

ALP (B-10): m-IgG2a BP-HRP 套装

sc-546115
200 µg Ab; 10 µg BP
RMB2662.00

ALP 抗体 (B-10) AC

sc-365765 AC
500 µg/ml, 25% agarose
RMB3129.00

ALP 抗体 (B-10) HRP

sc-365765 HRP
200 µg/ml
RMB2377.00

ALP 抗体 (B-10) FITC

sc-365765 FITC
200 µg/ml
RMB2482.00

ALP 抗体 (B-10) PE

sc-365765 PE
200 µg/ml
RMB2580.00

ALP 抗体 (B-10) Alexa Fluor® 488

sc-365765 AF488
200 µg/ml
RMB2685.00

ALP 抗体 (B-10) Alexa Fluor® 546

sc-365765 AF546
200 µg/ml
RMB2685.00

ALP 抗体 (B-10) Alexa Fluor® 594

sc-365765 AF594
200 µg/ml
RMB2685.00

ALP 抗体 (B-10) Alexa Fluor® 647

sc-365765 AF647
200 µg/ml
RMB2685.00

ALP 抗体 (B-10) Alexa Fluor® 680

sc-365765 AF680
200 µg/ml
RMB2685.00

ALP 抗体 (B-10) Alexa Fluor® 790

sc-365765 AF790
200 µg/ml
RMB2685.00

ALP抗体(B-10): sc-365765 この製品は IHCに1次抗体として用いる事ができますか?

Asked by: SWD35
Thank you for your question. This antibody is recommended for immunostaining experiments on cells and frozen sections, paraffin sections are not warranted. Thank you. ご質問ありがとうございます。この抗体は細胞や凍結切片の免疫染色に推奨されており、パラフィン切片は保証されていません。よろしくお願いします。
Answered by: Jiajun Z
Date published: 2025-08-07

I plan to perform IHC-P on decalcified paraffin-embedded rat vertebral bone using your Anti-ALP (B-10) antibody (sc-365765). Could you advise the recommended dilution, incubation time, and any special considerations for this tissue type?

Asked by: SWD35
Thank you for your question, sorry, this antibody is not recommended for immunostaining experiments on paraffin sections. Thank you.
Answered by: Jiajun Z
Date published: 2025-08-07

请问抗体建议稀释比例是多少呢?

Asked by: Anonymous
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

What is the recommended fixation method for immunofluorescence staining with ALP (B-10): sc-365765 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from greatI bought it one month ago. it's good. i am happy buy it
Date published: 2022-03-27
Rated 5 out of 5 by from good signalThe antibody works very well in immunofluorescence (1:200) on mesenchymal stem cells fixed with 4% PFA. Moreover the ALP-sample works good at the ratio of 1:500 in WB in the whole cell lisate.
Date published: 2020-01-08
Rated 5 out of 5 by from Excellent Immunofluorescence SignalImmunofluorescence (1:200) works very well on cryosections from 4% PFA fixed embryonic mouse brain tissue. 3-5 min retrieval with citric buffer (PH6.0) is necessary.
Date published: 2017-11-09
Rated 5 out of 5 by from WB excellentWork good at the ratio of 1:500 in WB. background is clear.
Date published: 2017-10-22
Rated 5 out of 5 by from This antibody has a good effect.The WB band is very beautiful, and the background is very clean.
Date published: 2017-09-19
Rated 5 out of 5 by from greatWestern blot use a dilution of 1:1000 . The signal was very powerful, we got specific band at 46kd and 54kda
Date published: 2017-09-19
Rated 5 out of 5 by from Good WBThis antibody was used to detect the expression of ALP, specific band in WB.
Date published: 2017-05-19
Rated 4 out of 5 by from 能用申请的小样筛选通路,可惜没趋势,不过条带很干净,感谢Santa,其他的抗体都能用,有点遗憾,哈哈!!
Date published: 2017-03-15
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ALP 抗体 (B-10) is rated 4.9 out of 5 by 12.
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