Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Alix Antibody (3A9) - Western Blotting - Image 356098
Alix 抗体 (3A9): sc-53538. Jurkat (A), NIH/3T3 (B), U-937 (C), 3T3-L1 (D) 和 K-562 (E) 全细胞裂解液中 Alix 表达的 Western 印迹分析.
Alix 抗体 (3A9): sc-53538. Jurkat (A), NIH/3T3 (B), U-937 (C), 3T3-L1 (D) 和 K-562 (E) 全细胞裂解液中 Alix 表达的 Western 印迹分析.

Alix 抗体 (3A9): sc-53538

4.0(12)
寫評論提問

说明书
  • Alix抗体(3A9)是小鼠单克隆IgG1 κ, 在70篇文献中引用，规格为200 µg/ml
  • Alix 抗体 (3A9) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 Xenopus来源的Alix
  • 抗Alix抗体（3A9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Alix (3A9): sc-53538的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Alix 抗体 (3A9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Alix 抗体 (3A9) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

Alix抗体（3A9）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）等方法检测小鼠、大鼠和人类样本中的Alix。Alix (3A9) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。ALG-2相互作用蛋白（通常称为Alix）在细胞过程中起着至关重要的作用，它促进多囊泡体内货物蛋白的分类和浓缩，这对于形成将蛋白质运送到其适当目的地的囊泡至关重要。这种分类机制对于维持细胞内稳态和调节细胞凋亡至关重要，因为Alix与参与这些过程的关键蛋白相互作用，包括ALG-2和程序性细胞死亡6。此外，Alix与运输所需的内体分拣复合物（ESCRT）蛋白（如Tsg101和CHMP4）的相互作用，凸显了Alix在胞吞途径和病毒芽生中的重要性，尤其是在HIV-1的背景下。抗Alix抗体（3A9）使研究人员能够深入了解Alix在细胞运输中的动态作用及其对癌症和病毒感染等各种疾病的影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Alix 抗体 (3A9) 参考文献:

  1. Alix和ALG-2在细胞内体和神经元死亡之间建立联系。  |  Mahul-Mellier, AL., et al. 2009. Biochem Soc Trans. 37: 200-3. PMID: 19143631
  2. Syndecan-syntenin-ALIX 调节外泌体的生物生成。  |  Baietti, MF., et al. 2012. Nat Cell Biol. 14: 677-85. PMID: 22660413
  3. ALIX- 和 ESCRT-III 依赖性地将四泛素分选到外泌体。  |  Larios, J., et al. 2020. J Cell Biol. 219: PMID: 32049272
  4. Alix是大脑突触中活动依赖性整体内吞作用所必需的。  |  Laporte, MH., et al. 2022. PLoS Biol. 20: e3001659. PMID: 35658004
  5. 转变医学教育中的多样性、公平性和包容性--梅奥诊所阿利克斯医学院。  |  Valencia, EM. 2022. Mayo Clin Proc. 97: 1592-1594. PMID: 36058569
  6. ESCRT蛋白ALIX通过酪氨酸磷酸化发生可逆相分离。  |  Elias, RD., et al. 2023. Sci Adv. 9: eadg3913. PMID: 37450591
  7. 胞吐作用介导的ALIX和ESCRT-III在细胞分裂期间向细胞间桥的转运。  |  Pust, S., et al. 2023. Cell Mol Life Sci. 80: 235. PMID: 37523003
  8. SUMO化激活的ALIX调节细胞外囊泡circTLCD4-RWDD3，促进非小细胞肺癌的淋巴转移。  |  Diao, X., et al. 2023. Signal Transduct Target Ther. 8: 426. PMID: 37925421
  9. ALIX和TSG101对于两种猪冠状病毒的细胞进入和复制至关重要。  |  Chen, X., et al. 2024. PLoS Pathog. 20: e1012103. PMID: 38489378
  10. 在生理和应激条件下，Alix在调节心肌细胞外泌体生物发生中起着重要作用。  |  Wang, X., et al. 2024. J Mol Cell Cardiol. 190: 35-47. PMID: 38593639

订购信息

产品名称产品编号规格价格数量收藏夹

Alix 抗体 (3A9)

sc-53538
200 µg/ml
RMB2377.00

Alix (3A9): m-IgG Fc BP-HRP 套装

sc-526824
200 µg Ab; 10 µg BP
RMB2662.00

Alix (3A9): m-IgG1 BP-HRP 套装

sc-532197
200 µg Ab; 20 µg BP
RMB2662.00

Alix 抗体 (3A9) AC

sc-53538 AC
500 µg/ml, 25% agarose
RMB3129.00

Alix 抗体 (3A9) HRP

sc-53538 HRP
200 µg/ml
RMB2377.00

Alix 抗体 (3A9) FITC

sc-53538 FITC
200 µg/ml
RMB2482.00

Alix 抗体 (3A9) PE

sc-53538 PE
200 µg/ml
RMB2580.00

Alix 抗体 (3A9) Alexa Fluor® 488

sc-53538 AF488
200 µg/ml
RMB2685.00

Alix 抗体 (3A9) Alexa Fluor® 546

sc-53538 AF546
200 µg/ml
RMB2685.00

Alix 抗体 (3A9) Alexa Fluor® 594

sc-53538 AF594
200 µg/ml
RMB2685.00

Alix 抗体 (3A9) Alexa Fluor® 647

sc-53538 AF647
200 µg/ml
RMB2685.00

Alix 抗体 (3A9) Alexa Fluor® 680

sc-53538 AF680
200 µg/ml
RMB2685.00

Alix 抗体 (3A9) Alexa Fluor® 790

sc-53538 AF790
200 µg/ml
RMB2685.00

which epitope of Alix does this antibody recognize? Thank you very much

Asked by: Emanuela
Thank you for your question. Alix Antibody (3A9): sc-53538 was raised against full-length human protein. No epitope mapping to determine the exact binding site has been performed for this antibody as of yet.
Answered by: Tech Support Europe
Date published: 2024-11-27

We would like to test Alix (3A9): sc-53538 antibody as a marker of exosomes in WB. How can we get 10 ug rate to prove it?

Asked by: Ewa1
Thank you for your question. Please contact our technical service team directly to make arrangements for a free sample of this antibody.
Answered by: Tech Support Europe
Date published: 2022-02-24

Hello! We would like to use the Alix (3A9) sc-53538 as an exosome marker (Western blot) in exosomes isolated from pig (Sus Scrofa). Does it work with pig? Thanks.

Asked by: ELZ_CAN
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext 1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-08-11

we want quotation of Alix Antibody (3A9): sc-53538

Asked by: indradev
Thank you for your question. For accurate availability, shipping and pricing information for a Santa Cruz product, or to receive a quote, please contact Customer Service or your local Distributor.
Answered by: Tech Service
Date published: 2019-09-05

Should this antibody be used under reduced or non-reduced conditions?

Asked by: OS EVs
Thank you for your question. We recommend using this mouse monoclonal antibody, Alix (3A9): sc-53538, for Western blot under reducing conditions. A link to our recommended protocol is provided below as a reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-10-04

What is the best secondary to use with Alix Antibody (3A9): sc-53538 in Western Blot?

Asked by: jenni
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-09
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_6
  • loc_zh_CN, sid_53538, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 102ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from No signal on TE-4 cells proteins.I tested this alix antibody on protein exctracted from TE-4 cells by western blot. Antibody was diluted 1:200 as suggested in BSA 5% and incubated o/n at 4°C. However, it didn't work and no signal was shown.
Date published: 2021-04-29
Rated 5 out of 5 by from Alix Antibdy (3A9) works pretty well in our systemI probed Aix in B6-derived tumors. The antibody was diluted in 1% milk at 1:500 ratio. In the last experiment, we got pretty clear bands
Date published: 2018-04-24
Rated 1 out of 5 by from WB培養細胞（H4細胞）のWesternで、20ugを泳動して1:200の希釈で用いて予想される100 kDaにバンドは確認されませんでした。過剰発現でも予想される位置にバンドは確認されませんでした。
Date published: 2018-04-06
Rated 5 out of 5 by from The antibody worked well in WBI suggest dilution this antibody at 1:1000 for WB, I'm satisfied with this antibody
Date published: 2017-09-01
Rated 5 out of 5 by from Outstanding WB and IHCGreat product for both western blot and IHC APPLICATIONS
Date published: 2017-01-07
Rated 5 out of 5 by from WB in mouse and humanTarget band observed in human and mouse cell lines in WB.
Date published: 2016-12-29
Rated 5 out of 5 by from Worked well for WB and IF using human sampleWorked well for WB and IF using human sample.
Date published: 2015-07-13
Rated 4 out of 5 by from Strong bands seen in WB using KStrong bands seen in WB using K-562 and U-937 nuclear extracts. -SCBT QC
Date published: 2015-05-10
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_53538, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 19ms
  • REVIEWS, PRODUCT
Alix 抗体 (3A9) is rated 4.0 out of 5 by 12.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_53538, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 108ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。