Date published: 2026-10-24

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Alix Antibody (2H12) - Western Blotting - Image 13633
Alix 抗体 (2H12): sc-53539. 核提取物 HeLa (A), Jurkat (B), K-562 (C), THP-1 (D) 和 U-937 (E) 中 Alix 表达的 Western 印迹分析.
Alix 抗体 (2H12): sc-53539. 核提取物 HeLa (A), Jurkat (B), K-562 (C), THP-1 (D) 和 U-937 (E) 中 Alix 表达的 Western 印迹分析.

Alix 抗体 (2H12): sc-53539

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说明书
  • Alix抗体(2H12)是小鼠单克隆IgG1 κ, 在11篇文献中引用，规格为200 µg/ml
  • 推荐用于 mouse, rat， human 和 Xenopus 来源的Alix WB, IP, IF 和 ELISA检测
  • 看 Alix (1A12): sc-53540，可查看有标记的 Alix 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Alix Antibody (2H12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Alix Antibody (2H12) 打包提供（请参阅下面的订购信息）。
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関連項目

ALG-2相互作用蛋白（Alix），也称为程序性细胞死亡6相互作用蛋白（PDCD6相互作用蛋白），是一种细胞质蛋白。 Alix与凋亡相关蛋白（ALG-2和PDCD6）和内吞作用调节因子CIN85相互作用。此外，Alix与运输所需的内体分选复合物（ESCRT）蛋白（Tsg101和CHMP4）相互作用，并且可以与HIV-1结合。内吞素（SH3P4、SH3P8和SH3P13）是改变膜曲率的酶，在包膜囊泡形成的早期和晚期步骤中是必需的，也与Alix结合。 Alix参与多囊体的货物蛋白的浓缩和分选，以将其整合到囊泡中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Alix 抗体 (2H12) 参考文献:

  1. 运输和发育信号：处于十字路口的Alix。  |  Mattei, S., et al. 2006. Eur J Cell Biol. 85: 925-36. PMID: 16766083
  2. NP需要ALIX/AIP1才能整合到Mopeia病毒Z诱导的类病毒颗粒中。  |  Shtanko, O., et al. 2011. J Virol. 85: 3631-41. PMID: 21248028
  3. 肝素酶激活了辛迪加-合成蛋白-ALIX外泌体途径。  |  Roucourt, B., et al. 2015. Cell Res. 25: 412-28. PMID: 25732677
  4. Alix 在人类胶质瘤细胞增殖中的作用。  |  Zhao, C., et al. 2016. Hum Pathol. 52: 110-8. PMID: 26980041
  5. 小鼠大脑的正常发育需要Alix。  |  Laporte, MH., et al. 2017. Sci Rep. 7: 44767. PMID: 28322231
  6. ALIX增加iPSC衍生的外泌体的蛋白质含量和保护功能。  |  Sun, R., et al. 2019. J Mol Med (Berl). 97: 829-844. PMID: 30944935
  7. 二聚体和单体支架蛋白Alix在调节F-肌动蛋白组装和外泌体货物装载方面的不同功能。  |  Qiu, X., et al. 2022. J Biol Chem. 298: 102425. PMID: 36030822
  8. 尿中Alix标准化外泌体程序性死亡配体1分析可精确监测尿路上皮癌。  |  Woo, HK., et al. 2024. Cancer Sci. 115: 1602-1610. PMID: 38480462
  9. 拟南芥CaLB1与ESCRT蛋白ALIX发生相分离，并调节自噬体成熟。  |  Mosesso, N., et al. 2024. Nat Commun. 15: 5188. PMID: 38898014
  10. 在EBV+鼻咽癌中靶向LMP1-ALIX轴可抑制免疫抑制性小细胞外囊泡分泌并增强抗肿瘤免疫力  |  He, F., et al. 2024. Cancer Commun (Lond). 44: 1391-1413. PMID: 39402748

订购信息

产品名称产品编号规格价格数量收藏夹

Alix 抗体 (2H12)

sc-53539
200 µg/ml
CNY2422.00

Alix (2H12): m-IgG Fc BP-HRP 套装

sc-536983
200 µg Ab; 10 µg BP
CNY2715.00

Alix (2H12): m-IgGκ BP-HRP 套装

sc-534162
200 µg Ab; 40 µg BP
CNY2715.00

Alix (2H12): m-IgG1 BP-HRP 套装

sc-544939
200 µg Ab; 20 µg BP
CNY2715.00

Are there any issues with using sc-53539: Alix (2H12) mouse monoclonal primary antibody on mouse samples?

Asked by: Germaine
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-53539 conjugates.
Answered by: Technical Support
Date published: 2017-03-07

What is the lower mw band with the nuclear extract.   Is that an Alix isoform or contaminate ?

Asked by: Roger Madison
Thank you for your question. We have not further characterized the lower molecular weight band observed in the nuclear extracts tested (HeLa, Jurkat, K-562, THP-1 and U-937) by Western blot with this Alix Antibody (2H12): sc-53539. However, this band is also observed with a different Alix antibody with a different epitope, sc-53540: Alix (1A12).
Answered by: Technical Support
Date published: 2016-12-20
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Rated 5 out of 5 by Trav11 from Good antibody for WBThe Alix Antibody (2H12): sc-53539 works well for Western blot applications. This antibody has also been cited several times for further verification.
Date published: 2017-01-18
Rated 4 out of 5 by FL from Worked with transfected lysate by WBWorked with transfected lysate by WB -SCBT QC
Date published: 2015-04-28
Rated 4 out of 5 by HC from Publishable data in WB using mouse brainPublishable data in WB using mouse brain tumor exosomes -SCBT Publication Review
Date published: 2015-03-14
Rated 5 out of 5 by LR from Bands seen in WB using HeLaBands seen in WB using HeLa, Jurkat, K-562, THP-1 and U-937 nuclear extracts. -SCBT QC
Date published: 2015-01-10
Rated 4 out of 5 by SF from Publishable data in IP using mouse C2C12(myoblastPublishable data in IP using mouse C2C12(myoblast cell line) -SCBT Publication Review
Date published: 2013-01-24
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Alix 抗体 (2H12) is rated 4.4 out of 5 by 5.
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