Date published: 2025-9-8

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ALCAM 抗体 (H-9): sc-74557

5.0(3)
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说明书
  • ALCAM抗体(H-9)是小鼠单克隆IgG2b κ, 在4篇文献中引用,规格为200 µg/ml
  • 免疫human来源ALCAM近N-terminus的28-135氨基酸
  • 推荐用于 mouse, rat 和 human 来源的ALCAM WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 ALCAM (B-6): sc-74558,可查看有标记的 ALCAM 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG2b BP-HRP 是ALCAM 抗体 (H-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与ALCAM 抗体 (H-9) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

活化的白细胞细胞粘附分子(ALCAM)在活化的白细胞T细胞、B细胞和单核细胞上表达,是免疫球蛋白超家族(IgSF)的成员,被鉴定为CD6配体。CD6是清除受体半胱氨酸富集蛋白超家族中的I型膜蛋白,在T细胞粘附和共刺激中起作用。ALCAM通过同种(ALCAM-ALCAM)和异种(ALCAM-CD6)相互作用介导胸腺细胞-胸腺上皮细胞粘附,涉及清除受体半胱氨酸富集蛋白超家族成员和免疫球蛋白超家族之间以前未描述的蛋白质-蛋白质相互作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ALCAM 抗体 (H-9) 参考文献:

  1. 活化的白细胞细胞粘附分子(ALCAM 或 CD166)可调节骨表型和造血。  |  Hooker, RA., et al. 2015. J Musculoskelet Neuronal Interact. 15: 83-94. PMID: 25730656
  2. 活化的白细胞细胞粘附分子刺激过敏性哮喘中的 T 细胞反应  |  Kim, MN., et al. 2018. Am J Respir Crit Care Med. 197: 994-1008. PMID: 29394080
  3. 活化白细胞细胞粘附分子调节特应性皮炎的 Th2 免疫反应  |  Oh, MS., et al. 2019. Allergy Asthma Immunol Res. 11: 677-690. PMID: 31332979
  4. 活化白细胞粘附分子(ALCAM)在癌症进展、侵袭、转移和复发中的作用:一种新型癌症干细胞标记物和肿瘤特异性预后标记物。  |  Darvishi, B., et al. 2020. Exp Mol Pathol. 115: 104443. PMID: 32380056
  5. ALCAM/CD166:细胞粘附、干性和癌症进展的多效性介质  |  Ferragut, F., et al. 2021. Cytokine Growth Factor Rev. 61: 27-37. PMID: 34272152
  6. 活化白细胞细胞粘附分子 (ALCAM)/CD166 在人类实体瘤中的临床和抗肿瘤价值  |  Yang, Y., et al. 2021. Cancers (Basel). 13: PMID: 34680335
  7. CD6/ALCAM 通路通过 T 细胞介导的反应促进狼疮肾炎。  |  Chalmers, SA., et al. 2022. J Clin Invest. 132: PMID: 34981775
  8. 胰腺癌中的活化白细胞细胞粘附分子(ALCAM)/CD166,与临床结果和血管栓塞的关键联系。  |  Yang, Y., et al. 2021. Am J Cancer Res. 11: 5917-5932. PMID: 35018233
  9. 活化白细胞细胞粘附分子(ALCAM)--胃肠道癌症腹膜转移的潜在 '种子 '和 '土壤 '受体  |  Yang, YM., et al. 2023. Int J Mol Sci. 24: PMID: 36614319
  10. 活化白细胞细胞粘附分子 ALCAM 在临床胃癌中的应用及患者对化疗的反应  |  Yang, YM., et al. 2023. Anticancer Res. 43: 1463-1475. PMID: 36974802

订购信息

产品名称产品编号规格价格数量收藏夹

ALCAM 抗体 (H-9)

sc-74557
200 µg/ml
RMB2377.00

ALCAM (H-9): m-IgG2b BP-HRP 套装

sc-548462
200 µg Ab; 10 µg BP
RMB2662.00

Hello, I am using ALCAM (B-6) fot detection in WB in U937 cells, used 1:500 dilution didn't give any signal, 1:100 very weak signal. Any explanation?

Asked by: marie_fra
Thank you for your question. Please contact our Technical Service Department for help in troubleshooting the antibody. Please call us toll-free at (800) 457-3801 option 2, or use the live chat on our website.
Answered by: Technical Support
Date published: 2018-11-19

I am using ALCAM (H-9): sc-74557 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Produced a clear band with human recombinant ALCAMProduced a clear band with human recombinant ALCAM. -SCBT QC
Date published: 2015-05-24
Rated 5 out of 5 by from Blow out band detected at expected mw in SKBlow out band detected at expected mw in SK-N-SH whole cell lysate. -SCBT QC
Date published: 2015-04-09
Rated 5 out of 5 by from Great immunoperoxidase membrane stainingGreat immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human prostate tissue. -SCBT QC
Date published: 2013-12-25
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ALCAM 抗体 (H-9) is rated 5.0 out of 5 by 3.
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