Date published: 2025-9-8

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AlaRS 抗体 (E-9): sc-165991

5.0(3)
寫評論提問

说明书
  • AlaRS抗体(E-9)是小鼠单克隆IgG2a κ, 在1篇文献中引用,规格为200 µg/ml
  • 针对氨基酸701-968,在AlaRS的human起源的C-terminus处进行映射
  • 推荐用于 mouse, rat 和 human 来源的AlaRS WB, IP, IF 和 ELISA检测
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是AlaRS Antibody (E-9) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与AlaRS Antibody (E-9) 打包提供(请参阅下面的订购信息)。

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関連項目

氨基酰基-tRNA合成酶的功能是催化tRNA被其相应的氨基酸进行氨基酰化,从而将氨基酸与含有tRNA的核苷酸三联体连接起来。II类tRNA合成酶是tRNA合成酶的一个高度保守的亚家族,它具有一个催化域,通过它与tRNA的氨基酸受体相互作用,还有一个第二域,通过它与tRNA分子的其余部分相互作用。AlaRS(丙氨酰-tRNA合成酶),也称为AARS,是一种968个氨基酸的细胞质蛋白,属于tRNA合成酶的II类亚家族。作为单体,AlaRS催化丙氨酸与相应tRNA(Ala)的ATP依赖性连接,从而产生丙氨酰-tRNA(Ala)。编码AlaRS的基因缺陷可能导致细胞内错误折叠蛋白的积累,最终导致细胞死亡。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

AlaRS 抗体 (E-9)

sc-165991
200 µg/ml
RMB2377.00

AlaRS (E-9): m-IgG Fc BP-HRP 套装

sc-537427
200 µg Ab; 10 µg BP
RMB2662.00

AlaRS (E-9): m-IgGκ BP-HRP 套装

sc-534686
200 µg Ab; 40 µg BP
RMB2662.00

AlaRS (E-9): m-IgG2a BP-HRP 套装

sc-548011
200 µg Ab; 10 µg BP
RMB2662.00

What is the recommended fixation method for immunofluorescence staining with AlaRS (E-9): sc-165991 monoclonal antibody?

Asked by: Professor Griffin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-01
  • y_2025, m_8, d_31, h_8CST
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Rated 5 out of 5 by from Good cytoplasmic staining with HeLaGood cytoplasmic staining with HeLa -SCBT QC
Date published: 2015-02-04
Rated 5 out of 5 by from Strong band at correct mw in MCF7Strong band at correct mw in MCF7, K-562 and Caki-1 whole cell lysates with minimal background bands -SCBT QC
Date published: 2015-01-05
Rated 5 out of 5 by from We wanted to detect alanyl tRNA synthetaseWe wanted to detect alanyl tRNA synthetase in different human cell lines and AlaRS (E9) was our choice of antibody from Santa Cruz. The polyclonal antibody worked really well at 1:2000 dilution (incubated overnight) and it was very specific. Though at higher exposure we saw some lower molecular weight bands appearing but that happens with any antibody. I have also used the antibody for immunofluorescence at 1:200 dilution and it worked well.
Date published: 2014-05-12
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AlaRS 抗体 (E-9) is rated 5.0 out of 5 by 3.
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