Date published: 2025-9-7

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Akt3 抗体 (EE-M14): sc-134254

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说明书
  • Akt3抗体(EE-M14)是小鼠单克隆IgG2a κ, 在8篇文献中引用,规格为100 µg/ml
  • 针对human重组Akt3蛋白的抗原性氨基酸
  • 推荐用于 mouse, rat 和 human 来源的Akt3 WB, IP 和 ELISA检测
  • 关于如何获取Akt3 (EE-M14): sc-134254免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成Akt3 抗体 (EE-M14)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

Akt3抗体(EE-M14)是一种IgG2a κ小鼠单克隆Akt3抗体,可通过WB、IP和ELISA检测来自小鼠、大鼠和人的Akt3。 Akt3抗体(EE-M14)可以是非偶联抗Akt3抗体形式存在。丝氨酸/苏氨酸激酶Akt家族包含几个成员,包括Akt1(也被称为PKB或RacPK)、Akt2(也被称为PKBβ或RacPK-β)和Akt 3(也被称为PKBγ或胸腺瘤病毒原癌基因3),它们与蛋白激酶A和C家族具有序列同源性,并由c-Akt原癌基因编码。 Akt家族的所有成员都有一个血小板-白细胞C激酶底物同源结构域。 Akt3在胰岛素的作用下在丝氨酸残基上被磷酸化。然而,通过不需要PH结构域存在的机制,蛋白激酶C的先前激活会抑制胰岛素对Akt3的激活。 Akt3在3T3-L1成纤维细胞、脂肪细胞和骨骼肌中表达,可能参与各种生物学过程,包括脂肪细胞和肌肉分化、糖原合成、葡萄糖摄取、细胞凋亡和细胞增殖。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Akt3 抗体 (EE-M14) 参考文献:

  1. 鉴定含有丝氨酸磷酸化调节位点的人类 Akt3(蛋白激酶 B γ)。  |  Nakatani, K., et al. 1999. Biochem Biophys Res Commun. 257: 906-10. PMID: 10208883
  2. 通过荧光原位杂交将编码Akt/蛋白激酶B家族成员的AKT3绘制到人类和啮齿动物的染色体上。  |  Murthy, SS., et al. 2000. Cytogenet Cell Genet. 88: 38-40. PMID: 10773662
  3. 磷脂酰肌醇-3-OH 激酶信号转导中的蛋白激酶 B(c-Akt)。  |  Burgering, BM. and Coffer, PJ. 1995. Nature. 376: 599-602. PMID: 7637810
  4. Akt 原癌基因编码的蛋白激酶是 PDGF 激活的磷脂酰肌醇 3- 激酶的靶标。  |  Franke, TF., et al. 1995. Cell. 81: 727-36. PMID: 7774014
  5. AH/PH 结构域介导的 Akt 分子间相互作用及其在 Akt 调节中的潜在作用。  |  Datta, K., et al. 1995. Mol Cell Biol. 15: 2304-10. PMID: 7891724
  6. 人胰腺细胞中 AKT2 的扩增以及反义 RNA 对 AKT2 表达和致瘤性的抑制。  |  Cheng, JQ., et al. 1996. Proc Natl Acad Sci U S A. 93: 3636-41. PMID: 8622988
  7. 蛋白激酶 C 可调节 3T3-L1 脂肪细胞在胰岛素刺激下增加的 Akt1 和 Akt3 活性。  |  Barthel, A., et al. 1998. Biochem Biophys Res Commun. 243: 509-13. PMID: 9480839
  8. 体内大鼠骨骼肌中的 Akt 激酶和 2-脱氧葡萄糖摄取:与胰岛素和运动有关的研究。  |  Turinsky, J. and Damrau-Abney, A. 1999. Am J Physiol. 276: R277-82. PMID: 9887206

订购信息

产品名称产品编号规格价格数量收藏夹

Akt3 抗体 (EE-M14)

sc-134254
100 µg/ml
RMB2504.00

For Western Blot, is it recommended to use denatured or non-denatured conditions with Akt3 (EE-M14): sc-134254 antibody?

Asked by: Randy McDonald
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 4 out of 5 by from Good for WBI've tested this antibody in western blotting and it worked well at 1:1000 dilution.
Date published: 2021-01-28
Rated 4 out of 5 by from Worked for WBGave a good band after longer exposure time. Used 1:200 for mouse tissue lysates
Date published: 2018-03-23
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 4 out of 5 by from a little weakthis antibody is specific , but the band is a little weak.
Date published: 2017-07-07
Rated 5 out of 5 by from Works well for Western with HSC-3 cellsWB: 1:200. Single strong band with HSC-3 and cal-27 cells.
Date published: 2017-01-10
Rated 5 out of 5 by from Single strong band in mouse NIH/3T3 samplesSingle strong band in mouse NIH/3T3 samples. -SCBT QC
Date published: 2015-03-24
Rated 5 out of 5 by from Works well for Western in MCF7 cellsWorks well for Western in MCF7 cells. -SCBT QC
Date published: 2015-02-26
Rated 5 out of 5 by from Great WB data in HeLa cellsGreat WB data in HeLa cells. -SCBT QC
Date published: 2015-02-06
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Akt3 抗体 (EE-M14) is rated 4.6 out of 5 by 9.
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