Date published: 2025-9-7

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Akt2 抗体 (F-7): sc-5270

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说明书
  • Akt2抗体(F-7)是小鼠单克隆IgG1 κ, 在56篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Akt2的C-terminus的氨基酸454-470之间
  • 抗-Akt2 抗体 (F-7) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 Akt2
  • 抗Akt2抗体(F-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Akt2 (F-7): sc-5270免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成Akt2 抗体 (F-7)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

Akt2抗体(F-7)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学等方法检测小鼠、大鼠和人类样本中的Akt2。Akt2抗体(F-7)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。Akt2是丝氨酸/苏氨酸激酶Akt家族的一员,在调节代谢、细胞生长和存活的细胞信号传导通路中起着至关重要的作用。具体来说,Akt2参与胰岛素信号传导通路,其中,磷脂酰肌醇3-激酶(PI3K)响应胰岛素和胰岛素样生长因子1(IGF-1)而激活。这种激活对于促进葡萄糖摄取和糖原合成至关重要,使Akt2成为维持体内葡萄糖稳态和能量平衡的关键因素。Akt2能够调节细胞凋亡和细胞增殖等多种细胞过程,这凸显了它在正常生理和疾病状态(如癌症和代谢紊乱)中的重要性。Akt2中的Pleckstrin同源结构域能够与磷脂相互作用,促进其聚集到细胞膜并随后激活,这对于信号级联的功能至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Akt2 抗体 (F-7) 参考文献:

  1. 鉴定含有丝氨酸磷酸化调节位点的人类 Akt3(蛋白激酶 B γ)。  |  Nakatani, K., et al. 1999. Biochem Biophys Res Commun. 257: 906-10. PMID: 10208883
  2. 通过荧光原位杂交将编码Akt/蛋白激酶B家族成员的AKT3绘制到人类和啮齿动物的染色体上。  |  Murthy, SS., et al. 2000. Cytogenet Cell Genet. 88: 38-40. PMID: 10773662
  3. 磷脂酰肌醇-3-OH 激酶信号转导中的蛋白激酶 B(c-Akt)。  |  Burgering, BM. and Coffer, PJ. 1995. Nature. 376: 599-602. PMID: 7637810
  4. Akt 原癌基因编码的蛋白激酶是 PDGF 激活的磷脂酰肌醇 3- 激酶的靶标。  |  Franke, TF., et al. 1995. Cell. 81: 727-36. PMID: 7774014
  5. AH/PH 结构域介导的 Akt 分子间相互作用及其在 Akt 调节中的潜在作用。  |  Datta, K., et al. 1995. Mol Cell Biol. 15: 2304-10. PMID: 7891724
  6. 人胰腺细胞中 AKT2 的扩增以及反义 RNA 对 AKT2 表达和致瘤性的抑制。  |  Cheng, JQ., et al. 1996. Proc Natl Acad Sci U S A. 93: 3636-41. PMID: 8622988
  7. 蛋白激酶 C 可调节 3T3-L1 脂肪细胞在胰岛素刺激下增加的 Akt1 和 Akt3 活性。  |  Barthel, A., et al. 1998. Biochem Biophys Res Commun. 243: 509-13. PMID: 9480839
  8. 体内大鼠骨骼肌中的 Akt 激酶和 2-脱氧葡萄糖摄取:与胰岛素和运动有关的研究。  |  Turinsky, J. and Damrau-Abney, A. 1999. Am J Physiol. 276: R277-82. PMID: 9887206

订购信息

产品名称产品编号规格价格数量收藏夹

Akt2 抗体 (F-7)

sc-5270
200 µg/ml
RMB2377.00

Akt2 抗体 (F-7) AC

sc-5270 AC
500 µg/ml, 25% agarose
RMB3129.00

Akt2 抗体 (F-7) HRP

sc-5270 HRP
200 µg/ml
RMB2377.00

Akt2 抗体 (F-7) FITC

sc-5270 FITC
200 µg/ml
RMB2482.00

Akt2 抗体 (F-7) PE

sc-5270 PE
200 µg/ml
RMB2580.00

Akt2 抗体 (F-7) Alexa Fluor® 488

sc-5270 AF488
200 µg/ml
RMB2685.00

Akt2 抗体 (F-7) Alexa Fluor® 546

sc-5270 AF546
200 µg/ml
RMB2685.00

Akt2 抗体 (F-7) Alexa Fluor® 594

sc-5270 AF594
200 µg/ml
RMB2685.00

Akt2 抗体 (F-7) Alexa Fluor® 647

sc-5270 AF647
200 µg/ml
RMB2685.00

Akt2 抗体 (F-7) Alexa Fluor® 680

sc-5270 AF680
200 µg/ml
RMB2685.00

Akt2 抗体 (F-7) Alexa Fluor® 790

sc-5270 AF790
200 µg/ml
RMB2685.00

Akt2 (F-7) 中和勝肽

sc-5270 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-5270 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Tech Service 3
Date published: 2017-01-27
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Rated 4 out of 5 by from Good antibodiesI got free sample from company. Antibodies works in dilution 1:1000 in WB.
Date published: 2018-06-10
Rated 4 out of 5 by from Good antibodiesI used this antibodies (dilution 1:1000) in detection AKT in cell lines. It works good.
Date published: 2018-04-29
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 5 out of 5 by from GOODIt is used to detect Akt2 antibody , It worked very well in WB, very satisfied
Date published: 2017-07-07
Rated 4 out of 5 by from Human tissue blottingAntibody was used in 1:1000 in human tissue by immunoblotting. The molecular weight of 56kda was detected as a specific band.
Date published: 2017-06-06
Rated 5 out of 5 by from good antibodyIt is used to detect Akt2 antibody in human samples, It worked very well in WB, very satisfied.
Date published: 2017-05-31
Rated 5 out of 5 by from western blotwestern blot with the protein extracted from HepG2 cell,the band is clear and specific.
Date published: 2017-05-28
Rated 5 out of 5 by from AKT2 produces clear and strong signals.IHC-P and IF work together with human samples to produce Western blot clear and intense signals for AKT2.
Date published: 2017-05-25
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Akt2 抗体 (F-7) is rated 4.7 out of 5 by 26.
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