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Akt2 Antibody (1G8C12) - Western Blotting - Image 18173 — Akt2 抗体 (1G8C12): sc-81148. MCF7 (A), HeLa (B), A-431 (C) 和 THP-1 (D) 全细胞裂解液中 Akt2 表达的 Western 印迹分析.

Akt2 抗体 (1G8C12): sc-81148. MCF7 (A), HeLa (B), A-431 (C) 和 THP-1 (D) 全细胞裂解液中 Akt2 表达的 Western 印迹分析.

Akt2 抗体 (1G8C12): sc-81148

Name: Akt2抗体 (1G8C12)
Brand: SCBIO
SKU: sc-81148
Price: 2377.0 CNY
Availability: InStock

4.7(7)

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说明书

Akt2抗体(1G8C12)是小鼠单克隆IgG_2b (kappa轻链)，规格为200 µg/ml
与重组蛋白结合，该重组蛋白与Akt2的2-150的氨基酸对应，来源于human
抗-Akt2 抗体 (1G8C12) 推荐用于 WB 和 IP，检测mouse, rat 和human 来源的 Akt2
抗Akt2抗体（1G8C12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取Akt2 (1G8C12): sc-81148的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
2b BP-HRP">m-IgG_2b BP-HRP和m-IgGκ BP-HRP是Akt2 Antibody (1G8C12) 适用于 WB 应用。的首选辅助检测试剂。这些试剂现与Akt2 Antibody (1G8C12) 打包提供（请参阅下面的订购信息）。

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関連項目

额外Akt抗体，包括Akt1, Akt2和Akt3

Akt2抗体（1G8C12）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）和免疫沉淀（IP）在小鼠、大鼠和人类样本中检测Akt2。Anti-Akt2抗体（1G8C12）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，可满足不同实验需求。Akt2是丝氨酸/苏氨酸激酶Akt家族的一员，在调节代谢、细胞生长和存活的细胞信号传导通路中发挥着关键作用。具体来说，Akt2受胰岛素和胰岛素样生长因子1（IGF-1）的影响，被磷脂酰肌醇3-激酶（PI3K）激活。这种激活对于调节葡萄糖摄取和糖原合成至关重要，使Akt2成为代谢调节和能量平衡的关键因素。Akt2能够促进细胞存活并抑制细胞凋亡，这凸显了它在各种生理过程（包括脂肪细胞分化和肌肉发育）中的重要性。了解Akt2的功能和调节机制对于探索代谢紊乱和癌症的治疗策略至关重要，因为经常观察到这种途径的失调。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor^® 是Molecular Probes Inc., OR., USA的商标

LI-COR^®和 Odyssey^® 是LI-COR Biosciences的注册商标

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
Akt2 抗体 (1G8C12)	sc-81148	200 µg/ml	RMB2377.00
Akt2 (1G8C12): m-IgGκ BP-HRP 套装	sc-521164	200 µg Ab, 40 µg BP	RMB2662.00
Akt2 (1G8C12): m-IgG_2b BP-HRP 套装	sc-548868	200 µg Ab; 10 µg BP	RMB2662.00
Akt2 抗体 (1G8C12) AC	sc-81148 AC	500 µg/ml, 25% agarose	RMB3129.00
Akt2 抗体 (1G8C12) HRP	sc-81148 HRP	200 µg/ml	RMB2377.00
Akt2 抗体 (1G8C12) FITC	sc-81148 FITC	200 µg/ml	RMB2482.00
Akt2 抗体 (1G8C12) PE	sc-81148 PE	200 µg/ml	RMB2580.00
Akt2 抗体 (1G8C12) Alexa Fluor^® 488	sc-81148 AF488	200 µg/ml	RMB2685.00
Akt2 抗体 (1G8C12) Alexa Fluor^® 546	sc-81148 AF546	200 µg/ml	RMB2685.00
Akt2 抗体 (1G8C12) Alexa Fluor^® 594	sc-81148 AF594	200 µg/ml	RMB2685.00
Akt2 抗体 (1G8C12) Alexa Fluor^® 647	sc-81148 AF647	200 µg/ml	RMB2685.00
Akt2 抗体 (1G8C12) Alexa Fluor^® 680	sc-81148 AF680	200 µg/ml	RMB2685.00
Akt2 抗体 (1G8C12) Alexa Fluor^® 790	sc-81148 AF790	200 µg/ml	RMB2685.00

can this be used for ihc ?

Asked by: atish b

Thank you for your question. Anti-Akt2 Antibody (1G8C12) is recommended for western blot and IP. If you would like to use the antibody for IHC here is our protocol. https://www.scbt.com/resources/protocols/immunoperoxidase-staining

Answered by: BlakeJ

Date published: 2025-05-14

What is the recommended starting dilution for Western blot for Akt2 (1G8C12): sc-81148 monoclonal antibody?

Asked by: Dr Ninau Qelp

Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-81148.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.

Answered by: Technical Support

Date published: 2017-02-27

Rated 4 out of 5 by agnes2007 from Good antibodiesI used this antibodies in WB in dilution 1:5000. Great working

Date published: 2018-07-04

Rated 4 out of 5 by Tsen from Good antibodyWorked well with western blotting at 1:500 dilution.

Date published: 2017-09-27

Rated 5 out of 5 by twinsmc from good antibodythis antibody was tested on human aortic smooth muscle cells, and minimal background was observed, leaving a sharp and specific band with predicted size.

Date published: 2017-09-26

Rated 5 out of 5 by yl527 from Good WB dataworked well in detecting Akt from hepG2 cell lysate

Date published: 2017-09-06

Rated 5 out of 5 by Like hao from Good WB data with Mouse liverwe used this antibody in Mouse liver，got the wonderful bands，clean，strong，signal，it is very better

Date published: 2017-01-10

Rated 5 out of 5 by MC from WB: Good data in KNRK cellsWB: Good data in KNRK cells. -SCBT QC

Date published: 2015-03-07

Rated 5 out of 5 by QZ from Fantastic WB data with MCF7Fantastic WB data with MCF7, HeLa, A-431 and THP-1 whole cell lysates. -SCBT QC

Date published: 2015-01-14

Akt2 抗体 (1G8C12) is rated 4.7 out of 5 by 7.

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Akt2 抗体 (1G8C12): sc-81148

Akt2 抗体 (1G8C12) 规格

替代产品名称: Akt2

产品克隆名称: 1G8C12

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG_2b kappa light chain

氨基酸: 2-150

抗原表位靶标: Akt2

抗原表位种属: human

产品反应性/检测: Akt2

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP

瓶的信息: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 0篇各种科学出版物的引文

Akt2 抗体 (1G8C12)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

優選的 Akt1/2/3 抗體是 Akt1/2/3 抗體 (5C10): sc-81434.