Date published: 2025-9-7
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AIRE-1 Antibody (C-2) - Western Blotting - Image 320284
AIRE-1 抗体 (C-2): sc-373703. 在 MIA PaCa-2 (A), U-251-MG (B) 和 SW-13 (C) 全细胞裂解液以及 A-431 (D) 和 Jurkat (E) 核提取物中 AIRE-1 表达的 Western 印迹分析.
AIRE-1 抗体 (C-2): sc-373703. 在 MIA PaCa-2 (A), U-251-MG (B) 和 SW-13 (C) 全细胞裂解液以及 A-431 (D) 和 Jurkat (E) 核提取物中 AIRE-1 表达的 Western 印迹分析.

AIRE-1 抗体 (C-2): sc-373703

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说明书
  • AIRE-1 抗体 C-2 是小鼠单克隆 IgG1 κ，AIRE-1抗体, 在9篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列246-545位于human物种的AIRE-1的C-terminus
  • AIRE-1 抗体 (C-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 AIRE-1 isoforms 1, 2 and 3
  • 抗AIRE-1抗体（C-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取AIRE-1 (C-2): sc-373703的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是AIRE-1 Antibody (C-2) for WB and IHC(P) applications. 的首选辅助检测试剂，这些试剂现与AIRE-1 Antibody (C-2) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

AIRE-1抗体（C-2）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的AIRE-1蛋白。AIRE-1 (C-2) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。AIRE-1蛋白（又称自身免疫调节蛋白）通过调节胸腺中的自身抗原表达在免疫系统中发挥着至关重要的作用，而胸腺中的自身抗原表达对于自身耐受性发育和自身免疫性疾病预防至关重要。AIRE-1定位于胸腺的髓质上皮细胞和单核细胞-树突状细胞中，负责协调胸腺正常环境建立所需的基因转录控制。这种定位确保了发育中的T细胞能够接触到各种自身抗原，从而在负选择过程中消除自身反应性T细胞。AIRE-1的突变会导致胸腺T细胞选择受损，从而导致自身免疫性疾病，如自身免疫性多发性内分泌病、念珠菌病、外胚层营养不良（APECED）。在研究中使用抗AIRE-1抗体（C-2）可以深入了解免疫耐受机制和AIRE-1功能障碍对自身免疫性疾病的影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

AIRE-1 抗体 (C-2) 参考文献:

  1. 散发型自身免疫性阿狄森氏病中细胞毒性T淋巴细胞抗原-4（CTLA-4）和自身免疫调节因子-1（AIRE-1）基因的关联分析。  |  Vaidya, B., et al. 2000. J Clin Endocrinol Metab. 85: 688-91. PMID: 10690877
  2. 自身免疫性肝炎。  |  Obermayer-Straub, P., et al. 2000. J Hepatol. 32: 181-97. PMID: 10728804
  3. 正常胸腺结构和负选择与自身免疫多内分泌病-念珠菌病-外胚层营养不良症（APECED）的缺陷基因 Aire 的表达有关。  |  Zuklys, S., et al. 2000. J Immunol. 165: 1976-83. PMID: 10925280
  4. 自身免疫调控蛋白的亚细胞定域。核靶向和转录激活结构域的表征。  |  Pitkänen, J., et al. 2001. J Biol Chem. 276: 19597-602. PMID: 11274163
  5. 在一个法国自身免疫性多内分泌病-念珠菌病-外胚层营养不良症家族中发现自身免疫调节剂（AIRE-1）基因的新型突变。  |  Saugier-Veber, P., et al. 2001. Eur J Endocrinol. 144: 347-51. PMID: 11275943
  6. 对患有阿狄森氏病、1型糖尿病、格雷夫斯病、桥本氏甲状腺炎以及APECED综合征的患者进行AIRE-1突变筛查。  |  Meyer, G., et al. 2001. Clin Endocrinol (Oxf). 54: 335-8. PMID: 11298085
  7. APECED 脾功能不全的自然病史--患者报告。  |  Starzyk, J., et al. 2001. J Pediatr Endocrinol Metab. 14: 443-9. PMID: 11327379
  8. 自身免疫调节剂 AIRE：自身免疫性肝炎与作为自身免疫性多腺综合征 1 型一部分的肝炎之间存在遗传差异的证据。  |  Vogel, A., et al. 2001. Hepatology. 33: 1047-52. PMID: 11343230
  9. AIRE-1（自身免疫调节因子1）作为胸腺诱导负选择的调节因子。  |  Park, Y., et al. 2003. Ann N Y Acad Sci. 1005: 431-5. PMID: 14679106
  10. 在一组自身免疫性多内分泌病-念珠菌病-外胚层营养不良患者中，对自身免疫调节因子-1信使核糖核酸进行新型内含子突变和另外两种新型AIRE基因突变分析。  |  Podkrajsek, KT., et al. 2005. J Clin Endocrinol Metab. 90: 4930-5. PMID: 15886230
  11. 46名1型糖尿病患儿和44名格雷夫斯病患儿中不存在AIRE-1基因的杂合K83E和R257X突变。  |  Iwama, S., et al. 2005. Clin Pediatr Endocrinol. 14: 17-21. PMID: 24790305
  12. 英国1型自身免疫性多发性内分泌病家族中自身免疫调节因子基因中常见的13个碱基对缺失。  |  Pearce, SH., et al. 1998. Am J Hum Genet. 63: 1675-84. PMID: 9837820

订购信息

产品名称产品编号规格价格数量收藏夹

AIRE-1 抗体 (C-2)

sc-373703
200 µg/ml
RMB2377.00

AIRE-1 (C-2): m-IgG Fc BP-HRP 套装

sc-527407
200 µg Ab; 10 µg BP
RMB2662.00

AIRE-1 (C-2): m-IgG1 BP-HRP 套装

sc-532780
200 µg Ab; 20 µg BP
RMB2662.00

AIRE-1 抗体 (C-2) AC

sc-373703 AC
500 µg/ml, 25% agarose
RMB3129.00

AIRE-1 抗体 (C-2) HRP

sc-373703 HRP
200 µg/ml
RMB2377.00

AIRE-1 抗体 (C-2) FITC

sc-373703 FITC
200 µg/ml
RMB2482.00

AIRE-1 抗体 (C-2) PE

sc-373703 PE
200 µg/ml
RMB2580.00

AIRE-1 抗体 (C-2) Alexa Fluor® 488

sc-373703 AF488
200 µg/ml
RMB2685.00

AIRE-1 抗体 (C-2) Alexa Fluor® 546

sc-373703 AF546
200 µg/ml
RMB2685.00

AIRE-1 抗体 (C-2) Alexa Fluor® 594

sc-373703 AF594
200 µg/ml
RMB2685.00

AIRE-1 抗体 (C-2) Alexa Fluor® 647

sc-373703 AF647
200 µg/ml
RMB2685.00

AIRE-1 抗体 (C-2) Alexa Fluor® 680

sc-373703 AF680
200 µg/ml
RMB2685.00

AIRE-1 抗体 (C-2) Alexa Fluor® 790

sc-373703 AF790
200 µg/ml
RMB2685.00

I want to use AIRE-1 (c-2) sc-373703AF488 antibody and FCM to visualize expression in human monocyte-derived Dendritic cells. Do I have to perform intracellular staining (fixation/permeabilization)?

Asked by: Alan
Thank you for your question. For assistance please contact Technical service. Please call 800-457-3801 or email [email protected].
Answered by: BlakeJ
Date published: 2025-04-11

Has this antibody also been tested for intranuclear flow cytometry purposes?

Asked by: Epithelian
Thank you for your question. sc-373703 AIRE-1 (C-2) has not been validated for flow cytometry.
Answered by: BlakeJ
Date published: 2025-03-27

Hello, What do you use as a negative control for AIRE-1, clone C-2? Would you be able to provide an aliquot for our lab? Thanks, Paige

Asked by: snow1187
Thank you for your question. We recommend using a normal mouse IgG as a negative control when using this antibody. We do not have a specific negative control cell line. Unfortunately we do not offer a sample of the normal mouse IgG, but we would be happy to send you a sample of the antibody, sc-373703. Please contact technical support by phone, email or chat and we'd be happy to get that out to you!
Answered by: Tech Service
Date published: 2017-05-24

I am using AIRE-1 (C-2): sc-373703 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jenniferc
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good antibodyThe antibody works very well in IHC on FFPE. The best dilution is 1:1500 using as antigen retrival EDTA buffer pH 8.0 in water bath at 98°C. The staining is strong and nuclear.
Date published: 2022-05-26
Rated 5 out of 5 by from Versatile antibody!This mouse monoclonal AIRE-1 Antibody (C-2) is a versatile antibody that has been validated in Western blotting and IHC(P) for mouse, rat and human samples.
Date published: 2017-01-31
Rated 5 out of 5 by from Good specificity in WB in MIA PaCaGood specificity in WB in MIA PaCa-2, U-251-MG and SW-13 whole cell lysates and A-431and Jurkat nuclear extracts. -SCBT QC
Date published: 2015-06-16
Rated 5 out of 5 by from Positive signal in WB in rat thymus tissue extractPositive signal in WB in rat thymus tissue extract. -SCBT QC
Date published: 2015-04-14
Rated 5 out of 5 by from Good signal strength in WB in AGood signal strength in WB in A-431, Jurkat and A549 nuclear extracts. -SCBT QC
Date published: 2015-04-04
Rated 5 out of 5 by from Strong band in WB in HepG2 whole cell lysateStrong band in WB in HepG2 whole cell lysate. -SCBT QC
Date published: 2015-03-23
Rated 5 out of 5 by from Beautiful immunofluorescence nuclear stainingBeautiful immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-03-07
Rated 5 out of 5 by from Robust signal in WB in BW5147Robust signal in WB in BW5147, F9 and MIA PaCa-2 whole cell lysates. -SCBT QC
Date published: 2015-02-22
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AIRE-1 抗体 (C-2) is rated 5.0 out of 5 by 8.
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