Date published: 2025-10-25
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AHA-1 Antibody (F-7) - Western Blotting - Image 65914
AHA-1 抗体 (F-7): sc-166610. 未转染 293T: sc-117752 (A), 小鼠 AHA-1 转染 293T: sc-118276 (B) 和 KNRK (C) 全细胞裂解液中 AHA-1 表达的 Western 印迹分析.
AHA-1 抗体 (F-7): sc-166610. 未转染 293T: sc-117752 (A), 小鼠 AHA-1 转染 293T: sc-118276 (B) 和 KNRK (C) 全细胞裂解液中 AHA-1 表达的 Western 印迹分析.

AHA-1 抗体 (F-7): sc-166610

5.0(4)
寫評論提問

说明书
  • AHA-1 抗体 F-7 是小鼠单克隆 IgG1 κ，AHA-1抗体, 在3篇文献中引用，规格为200 µg/ml
  • 针对氨基酸249-338的抗原，在AHA-1的C-terminus处映射，human来源
  • AHA-1 抗体 (F-7) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 AHA-1
  • 抗AHA-1抗体（F-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是AHA-1 Antibody (F-7) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与AHA-1 Antibody (F-7) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

AHA-1抗体（F-7）是一种IgG1 κ小鼠单克隆AHA-1抗体（也称为AHA-1抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的AHA-1蛋白。AHA-1抗体（F-7）既可作为非偶联抗AHA-1抗体形式提供，也可作为多种偶联形式的抗AHA-1抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。AHA-1能刺激酵母和人类HSP 90的固有ATPase活性，并与囊泡性口炎病毒糖蛋白的细胞质尾部相互作用。AHA-1通过影响"ATP盖"的构象状态和随后的N端二聚化来调节HSP 90。当HSP 90水平受到限制时，它对非最佳生长条件下的细胞活力至关重要。AHA-1是一种胞质蛋白，可能与内质网发生短暂的相互作用。它可以影响内质网到高尔基体转运的一个步骤。AHA-1在多种组织中表达，包括脑、心脏、骨骼肌和肾脏，在肝脏和胎盘中的表达水平较低。它由热休克和HSP 90抑制剂17-demethoxygeldanamycin处理所诱导。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

AHA-1 抗体 (F-7) 参考文献:

  1. 人类下丘脑-垂体-肾上腺轴的基因表达谱分析和全长 cDNA 克隆。  |  Hu, RM., et al. 2000. Proc Natl Acad Sci U S A. 97: 9543-8. PMID: 10931946
  2. 克隆CD34+造血干细胞/祖细胞中表达的300个以前未定义的基因的cDNA，并对其开放阅读框进行功能分析。  |  Zhang, QH., et al. 2000. Genome Res. 10: 1546-60. PMID: 11042152
  3. p38：一种能与水泡性口炎病毒糖蛋白结合的新型蛋白质。  |  Sevier, CS. and Machamer, CE. 2001. Biochem Biophys Res Commun. 287: 574-82. PMID: 11554768
  4. 应激调控的辅助伴侣 aha1 激活 hsp90 的 ATPase 活性。  |  Panaretou, B., et al. 2002. Mol Cell. 10: 1307-18. PMID: 12504007
  5. Aha1 与 Hsp90 的中间结构域结合，有助于激活客户蛋白，并刺激分子伴侣的 ATPase 活性。  |  Lotz, GP., et al. 2003. J Biol Chem. 278: 17228-35. PMID: 12604615

订购信息

产品名称产品编号规格价格数量收藏夹

AHA-1 抗体 (F-7)

sc-166610
200 µg/ml
RMB2377.00

AHA-1 (F-7): m-IgG Fc BP-HRP 套装

sc-529004
200 µg Ab; 10 µg BP
RMB2662.00

AHA-1 (F-7): m-IgGκ BP-HRP 套装

sc-521674
200 µg Ab, 40 µg BP
RMB2662.00

AHA-1 (F-7): m-IgG1 BP-HRP 套装

sc-543262
200 µg Ab; 20 µg BP
RMB2662.00

AHA-1 抗体 (F-7) AC

sc-166610 AC
500 µg/ml, 25% agarose
RMB3129.00

AHA-1 抗体 (F-7) HRP

sc-166610 HRP
200 µg/ml
RMB2377.00

AHA-1 抗体 (F-7) FITC

sc-166610 FITC
200 µg/ml
RMB2482.00

AHA-1 抗体 (F-7) PE

sc-166610 PE
200 µg/ml
RMB2580.00

AHA-1 抗体 (F-7) Alexa Fluor® 488

sc-166610 AF488
200 µg/ml
RMB2685.00

AHA-1 抗体 (F-7) Alexa Fluor® 546

sc-166610 AF546
200 µg/ml
RMB2685.00

AHA-1 抗体 (F-7) Alexa Fluor® 594

sc-166610 AF594
200 µg/ml
RMB2685.00

AHA-1 抗体 (F-7) Alexa Fluor® 647

sc-166610 AF647
200 µg/ml
RMB2685.00

AHA-1 抗体 (F-7) Alexa Fluor® 680

sc-166610 AF680
200 µg/ml
RMB2685.00

AHA-1 抗体 (F-7) Alexa Fluor® 790

sc-166610 AF790
200 µg/ml
RMB2685.00

Why is the band sometimes at 50 KD sometimes at 43 KD and sometimes not visible at all in YOUR own slides of the WB?

Asked by: I_dont_trust_this_AB
Thank you for your question. AHA-1 has a predicted molecular weight of 38 kDa. We typically observe this in the whole cell lysates tested at ~43 kDa when using our Cruz Marker Molecular Weight Standard (sc-2035). There is one published Western blot on our website where we instead used our Prestained Broad Range Markers (sc-2361) where the signal detected in the mouse AHA-1 transfected 293T whole cell lysate is observed slightly below the 50 kDa prestained marker, so close to 43 kDa. The difference in the observed molecular weight is due to the different molecular weight marker standards used. Please note that we do not always expect to detect any signal in the non-transfected 293T whole cell lysates. We have tested this AHA-1 antibody for specificity against different human and mouse AHA-1 transfected 293T whole cell lysates. In some cases, depending on the testing conditions (e.g., antibody/antigen concentration and exposure time) we may detect a signal in the non-tranfected control. However, the goal with the published data is to show reactivity with the overexpressed protein.
Answered by: Technical Support
Date published: 2019-03-08

I need to use an antibody to detect the protein in samples of equine origin. Do you know if sc-166610: AHA-1 (F-7) mouse monoclonal antibody will work?

Asked by: DefinitelyNotMatt
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 5 out of 5 by from Good specificity for AHA-1 in Western BlotThis antibody consistently detects over-expressed mouse AHA-1 in transfected lysate sc-118276. Shown with Cruz Marker molecular weight standards, sc-2035. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from WBできれいに検出できました抗体はWBに使用しました。サンプルはJurkatとMT-2細胞株。抗体の希釈倍率は1000倍。ブロッキング剤は市販のブロッキング剤または5%スキムミルクを使用しました。どちらのブロッキング剤を使用してもバックグラウンドはかなり低く、きれいなバンドが検出されました。
Date published: 2022-05-10
Rated 5 out of 5 by from worked wellThis antibody worked quite well at a dilution of 1:1000. The 293T cells were transfected with HSP90 and AHA-1 was detected clearly in HSP90 IP sample and Input.
Date published: 2017-08-08
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AHA-1 抗体 (F-7) is rated 5.0 out of 5 by 4.
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