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Agrin Antibody (D-2) - Western Blotting - Image 355505
Agrin 抗体 (D-2): sc-374117. EOC 20 (A) 和 NIH/3T3 (B) 全细胞裂解液中 Agrin 表达的 Western 印迹分析.
Agrin 抗体 (D-2): sc-374117. EOC 20 (A) 和 NIH/3T3 (B) 全细胞裂解液中 Agrin 表达的 Western 印迹分析.

Agrin 抗体 (D-2): sc-374117

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说明书
  • Agrin 抗体 D-2 是小鼠单克隆 IgM κ，Agrin抗体, 在12篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于rat物种的Agrin的C-terminus附近的氨基酸1913-1942之间
  • 推荐用于 mouse, rat 和 human 来源的Agrin WB, IP, IF, IHC(P) 和 ELISA检测; 也和以下物种反应，包括： 和 equine, canine and bovine
  • 另提供琼脂糖偶联抗体，规格为琼脂糖装，可用于IP实验；HRP 偶联抗体用于WB, IHC(P)和ELISA实验；以及藻红蛋白、FITC、Alexa Fluor® 488 或Alexa Fluor® 647 偶联抗体，规格为用于IF、IHC(P)和FCM实验
  • 关于如何获取Agrin (D-2): sc-374117的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Agrin 抗体 (D-2)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Agrin抗体（D-2）是一种IgM κ小鼠单克隆Agrin抗体（也称为Agrin蛋白聚糖抗体、CMSPPD抗体或CMS8抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Agrin蛋白。Agrin抗体（D-2）既可以是非偶联的抗Agrin抗体形式，也可以是多种偶联形式的抗Agrin抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Agrin是一种位于肌肉细胞基底膜的分子，可指导突触后分化的关键事件。最值得注意的是，Agrin负责乙酰胆碱受体（AChR）在细胞表面的聚集以及它们在神经肌肉接头的定位。已经发现了多种来自选择性mRNA剪接的Agrin变体。在羧基末端具有指定为"y"和"z"的两个插入位点的Agrin剪接形式对AChR具有高亲和力，而缺少这些插入的剪接形式与AChR的关联较弱。据推测，肌肉α-肌营养不良聚糖是聚集素聚集活性的受体；然而，这是一个有争议的问题。酪氨酸磷酸化已被认为是AChR聚集所需的早期步骤。有趣的是，已经发现了一种独特的受体酪氨酸激酶，称为MuSK，它与Agrin相互作用并特异性定位于发育中的肌肉。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Agrin 抗体 (D-2) 参考文献:

  1. 细胞外基质蛋白agrin促进小鼠心脏再生  |  Bassat, E., et al. 2017. Nature. 547: 179-184. PMID: 28581497
  2. 对Agrin/LRP4/MuSK信号传导复合物组装的结构研究。  |  Xie, T., et al. 2023. Proc Natl Acad Sci U S A. 120: e2300453120. PMID: 37252960
  3. 骨骼肌Agrin的缺乏导致小鼠年龄相关性肌少症的发病。  |  Chen, J., et al. 2024. Cell Death Dis. 15: 201. PMID: 38461287
  4. 海马星形胶质细胞中的Agrin-Lrp4通路通过腺苷信号抑制颞叶癫痫的发展。  |  Liu, ZY., et al. 2024. Cell Biosci. 14: 66. PMID: 38783336
  5. 哺乳动物肌肉中 Agrin 诱导的乙酰胆碱受体集群需要酪氨酸磷酸化。  |  Ferns, M., et al. 1996. J Cell Biol. 132: 937-44. PMID: 8603924
  6. 胰凝乳蛋白的交替剪接改变了其与肝素, 肌营养蛋白和假定的胰凝乳蛋白受体的结合。  |  Gesemann, M., et al. 1996. Neuron. 16: 755-67. PMID: 8607994
  7. Agrin 与α-肌收缩蛋白的结合。诱导乙酰胆碱受体聚类所需的 Agrin 区域与 alpha-肌收缩蛋白结合区域重叠，但并不相同。  |  Hopf, C. and Hoch, W. 1996. J Biol Chem. 271: 5231-6. PMID: 8617807
  8. 阿格林在路口发出信号。  |  Slater, CR. 1996. Nature. 381: 478-9. PMID: 8632818
  9. Agrin 通过 MuSK 受体复合物发挥作用。  |  Glass, DJ., et al. 1996. Cell. 85: 513-23. PMID: 8653787
  10. 胰凝乳蛋白缺陷突变小鼠的神经肌肉突触发生缺陷  |  Gautam, M., et al. 1996. Cell. 85: 525-35. PMID: 8653788
  11. 神经拮抗素能激活 C2 肌细胞中的高亲和性受体，而这种受体对肌肉拮抗素没有反应。  |  Bowen, DC., et al. 1996. J Neurosci. 16: 3791-7. PMID: 8656273
  12. agrin的交替剪接调节其与肝素α-肌张力蛋白和细胞表面的结合。  |  O'Toole, JJ., et al. 1996. Proc Natl Acad Sci U S A. 93: 7369-74. PMID: 8693000

订购信息

产品名称产品编号规格价格数量收藏夹

Agrin 抗体 (D-2)

sc-374117
200 µg/ml
RMB2377.00

Agrin 抗体 (D-2) AC

sc-374117 AC
500 µg/ml, 25% agarose
RMB3129.00

Agrin 抗体 (D-2) Alexa Fluor® 488

sc-374117 AF488
200 µg/ml
RMB2685.00

Agrin 抗体 (D-2) Alexa Fluor® 647

sc-374117 AF647
200 µg/ml
RMB2685.00

Agrin 抗体 (D-2) FITC

sc-374117 FITC
200 µg/ml
RMB2482.00

Agrin 抗体 (D-2) HRP

sc-374117 HRP
200 µg/ml
RMB2377.00

Agrin (D-2) 中和勝肽

sc-374117 P
100 µg/0.5 ml
RMB511.00

Agrin 抗体 (D-2) PE

sc-374117 PE
200 µg/ml
RMB2580.00

The epitope is somewhere within the LG3 domain of agrin, is this specific to the agrin LG3 domain? (there are a number of other proteins/ proteoglycans containing this domain)

Asked by: VEC4291
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-03-18

I used m-IgGκ BP-HRP: sc-516102 for agrin WB detection in human EC cell line HUVEC and mouse primary lung EC cells. I found one really week band at 95-130 kDa. Is it the right band?

Asked by: FICT
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

Can sc-374117 antibody recognize the 22-kDa fragment of agrin (CAF) by western blotting?

Asked by: MaPD
Thank you for your question. Agrin Antibody (D-2): sc-374117 has been raised against amino acids 1913-1942, which are present in the Agrin C-terminal 22 kDa fragment that corresponds to amino acids 1864-2068. The antibody is also specific against this fragment.
Answered by: Tech Support Europe
Date published: 2020-08-25

Can anti-mouse IgG secondary antibody be used to detect this primary antibody in WB?

Asked by: Wayneman
Thank you for your question. Agrin Antibody (D-2) is a mouse monoclonal IgM (kappa light chain). We would recommend using our m-IgGκ BP-HRP: sc-516102 for detection of this mouse IgM primary antibody in Western blot. Our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP) is confirmed to detect mouse IgG and mouse IgM (kappa light chain type).
Answered by: Technical Support
Date published: 2020-08-26

Please, what is the catalogue number of the antibody sc-374117 available conjugated to agarose for IP?

Asked by: AdrianaPL
Thank you for your question. The catalog number for the agarose conjugated antibody is Agrin (D-2): sc-374117 AC.
Answered by: Technical Support
Date published: 2018-12-05

Does this work on frozen mouse and frozen human tissue? For IHC and IF

Asked by: totoro
Thank you for your question. We recommend this antibody for IF, which stands for immunofluorescence or immunohistochemistry on fresh or frozen tissues or cells fixed in acetone or methanol. We also recommend the antibody for IHC(P), which stands for immunohistochemistry on formalin-fixed paraffin-embedded tissue sections.
Answered by: Technical Support
Date published: 2018-12-01

What is the exact epitope recognized by Agrin (D-2): sc-374117?

Asked by: TinTin
Agrin (D-2): sc-374117 is raised against amino acids 1913-1942 of Agrin of rat origin. The exact epitope location has not been determined.
Answered by: Technical Support 8
Date published: 2017-01-19
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Rated 4 out of 5 by from Great featuresI bought this one and a half months ago and have found this primary antibody well performed with western blots.
Date published: 2021-08-19
Rated 5 out of 5 by from Beautiful antibodyI originally ordered this as a sample and couldn't have been happier with the results. I use this antibody at a 1:200 dilution often for IF work (paraffin sections and fixed cultured cells) in mouse, rat and human tissue and it is absolutely stunning. It consistently produces extremely crisp, clear images. Very grateful that it exists!
Date published: 2019-07-10
Rated 5 out of 5 by from 挺好用的很好用的产品，从过完年就开始购买，使用，没有让我失望过，发货速度也很快，基本隔天到，已经连续购买三个了，棒棒哒
Date published: 2018-04-11
Rated 5 out of 5 by from yes good我两个月前购买了此产品，为此感到很高兴! 实验做出来都是好产品，希望做出更好的产品来来来来来来来来来
Date published: 2017-09-27
Rated 3 out of 5 by from not sure the band is correctIn HT-29 cells, I got a band around 120 KDa using this antibody
Date published: 2017-07-08
Rated 5 out of 5 by from It works well for cell lysate and supernatentI tried this antibody in WB for cell lysate and supernatent from invasive melanoma cells. It works very well with a clear band.
Date published: 2017-04-07
Rated 5 out of 5 by from Wow !! Superb dataStron reagent for multiple applications including WB, IF and IHC staining
Date published: 2017-02-03
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of Agrin expression in EOC 20 and NIH/3T3 whole cell lysates.
Date published: 2017-01-17
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Agrin 抗体 (D-2) is rated 4.7 out of 5 by 10.
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