Date published: 2025-9-11

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Adenovirus-2/5 E1A 抗体 (M73): sc-25

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说明书
  • Adenovirus-2/5 E1A 抗体 M73 是小鼠单克隆 IgG2a κ,Adenovirus-2/5 E1A抗体, 在101篇文献中引用,规格为200 µg/ml
  • 针对细菌trpE-E1A融合蛋白
  • Adenovirus-2/5 E1A 抗体 (M73) 推荐用于 WB, IP 和 IF,检测Adenovirus-2 和Adenovirus-5 来源的 E1A antigens
  • 抗Adenovirus-2/5 E1A抗体(M73)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Adenovirus-2/5 E1A (M73): sc-25免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是Adenovirus-2/5 E1A Antibody (M73) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Adenovirus-2/5 E1A Antibody (M73) 打包提供(请参阅下面的订购信息)。

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関連項目

腺病毒-2/5 E1A 抗体(M73)是一种小鼠单克隆 IgG2a kappa 轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)和免疫荧光(IF)检测腺病毒-2 和腺病毒-5 源的腺病毒-2/5 E1A 蛋白。抗腺病毒-2/5 E1A抗体(M73)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。E1A蛋白在腺病毒感染的早期阶段起着至关重要的作用,因为E1A对于调节病毒基因表达和宿主细胞转化至关重要。具体来说,E1A与视网膜母细胞瘤(Rb)蛋白相互作用,后者是细胞周期的关键调节因子,可使病毒绕过细胞生长控制并促进细胞增殖。这种相互作用对于腺病毒有效劫持宿主细胞机制至关重要,从而增强病毒复制和发病机制。此外,E1A能够调节病毒和细胞基因的转录,这凸显了E1A在病毒生命周期中的重要性,以及作为治疗干预靶标的潜力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Adenovirus-2/5 E1A 抗体 (M73) 参考文献:

  1. 腺病毒 2 晚期 mRNA 5' 末端的剪接片段。  |  Berget, SM., et al. 1977. Proc Natl Acad Sci U S A. 74: 3171-5. PMID: 269380
  2. 通过原生质体融合将功能性腺病毒 E1A 转录激活蛋白转移到哺乳动物细胞中。  |  Ferguson, B., et al. 1986. J Biol Chem. 261: 14760-3. PMID: 2945820
  3. 溶瘤腺病毒 5 载体在癌症治疗中的载体和转导:关注纤维嵌合和镶嵌、hexon 和 pIX。  |  Stepanenko, AA. and Chekhonin, VP. 2018. Virus Res. 257: 40-51. PMID: 30125593
  4. 表达 SARS-CoV-2 蛋白质的腺病毒 5 载体。  |  Bachus, S., et al. 2022. mSphere. 7: e0099821. PMID: 35224978
  5. 肥胖患者的腺病毒-5 和腺病毒-37 血清阳性反应。  |  Bostan, NG., et al. 2024. Iran J Microbiol. 16: 684-688. PMID: 39534296
  6. 一种细胞磷蛋白的分子克隆和特性鉴定,该磷蛋白与腺病毒 E1A 的保守 C 终止子结构域相互作用,参与对致癌转化体的负调控。  |  Schaeper, U., et al. 1995. Proc Natl Acad Sci U S A. 92: 10467-71. PMID: 7479821
  7. 腺病毒 2 晚期信使核糖核酸 5'末端的核苷酸序列。  |  Lockard, RE., et al. 1979. J Biol Chem. 254: 587-90. PMID: 762082
  8. 评估正常人和囊性纤维化患者呼吸道上皮细胞中是否存在与使用 E1a 腺病毒载体对囊性纤维化呼吸道表现进行基因治疗相关的腺病毒 E1a 序列。  |  Eissa, NT., et al. 1994. Hum Gene Ther. 5: 1105-14. PMID: 7833370
  9. 腺病毒 2/5 E1a 蛋白 C 末端的一个区域需要与细胞磷蛋白结合,对 T24-ras 介导的转化体、肿瘤发生和转移的负调控非常重要。  |  Boyd, JM., et al. 1993. EMBO J. 12: 469-78. PMID: 8440238
  10. 一种新型 C 终止子结合蛋白(CTBP2)与腺病毒 E1A 结合蛋白 CTBP1 关系密切,并映射到人类染色体 21q21.3 上。  |  Katsanis, N. and Fisher, EM. 1998. Genomics. 47: 294-9. PMID: 9479502

订购信息

产品名称产品编号规格价格数量收藏夹

Adenovirus-2/5 E1A 抗体 (M73)

sc-25
200 µg/ml
RMB2377.00

Adenovirus-2/5 E1A (M73): m-IgG Fc BP-HRP 套装

sc-528090
200 µg Ab; 10 µg BP
RMB2662.00

Adenovirus-2/5 E1A (M73): m-IgGκ BP-HRP 套装

sc-520387
200 µg Ab, 40 µg BP
RMB2662.00

Adenovirus-2/5 E1A (M73): m-IgG2a BP-HRP 套装

sc-547020
200 µg Ab; 10 µg BP
RMB2662.00

Adenovirus-2/5 E1A 抗体 (M73) AC

sc-25 AC
500 µg/ml, 25% agarose
RMB3129.00

Adenovirus-2/5 E1A 抗体 (M73) HRP

sc-25 HRP
200 µg/ml
RMB2377.00

Adenovirus-2/5 E1A 抗体 (M73) FITC

sc-25 FITC
200 µg/ml
RMB2482.00

Adenovirus-2/5 E1A 抗体 (M73) PE

sc-25 PE
200 µg/ml
RMB2580.00

Adenovirus-2/5 E1A 抗体 (M73) Alexa Fluor® 488

sc-25 AF488
200 µg/ml
RMB2685.00

Adenovirus-2/5 E1A 抗体 (M73) Alexa Fluor® 546

sc-25 AF546
200 µg/ml
RMB2685.00

Adenovirus-2/5 E1A 抗体 (M73) Alexa Fluor® 594

sc-25 AF594
200 µg/ml
RMB2685.00

Adenovirus-2/5 E1A 抗体 (M73) Alexa Fluor® 647

sc-25 AF647
200 µg/ml
RMB2685.00

Adenovirus-2/5 E1A 抗体 (M73) Alexa Fluor® 680

sc-25 AF680
200 µg/ml
RMB2685.00

Adenovirus-2/5 E1A 抗体 (M73) Alexa Fluor® 790

sc-25 AF790
200 µg/ml
RMB2685.00

ヒト組織(ホルマリン固定・パラフィン包埋組織)に交差性があるか教えてください。

Asked by: ZONORIN
Thank you for your question. Please contact our Technical Service Department and we will be happy to assist you. You may call us directly at 001 800-1338-3838, email at [email protected], or contact us by live chat. お問い合わせを頂きまして、誠にありがとうございました。お手数ですが、電話 001 800-1338-3838、メール[email protected]またはライブチャットで、当社のテクニカルサービスまでお問い合わせください。その他の不明点等がございましたら、またご連絡ください。
Answered by: Technical Support 8
Date published: 2018-12-27

How much protein do you recommend loading on the gel for Western blot detection using Adenovirus-2/5 E1A (M73): sc-25?

Asked by: jenni
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support 3
Date published: 2017-02-08
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Rated 5 out of 5 by from Ad5 MOI30 24hpi WorksWe ordered this 7 months ago, tested under the following conditions- infected HeLa cells with Ad5 at MOI 30 24 hours post infection and performed a 1 to 1000 dilution. A 10 second exposure was enough to confirm the presence of E1a in whole cell lysates.
Date published: 2018-06-14
Rated 5 out of 5 by from Very popular choice!This monoclonal Adenovirus-2/5 E1A Antibody (M73) is a very popular choice for Western blotting applications and has been featured in over 30 publications.
Date published: 2017-01-27
Rated 5 out of 5 by from Western blot effect is goodClean band in western blot analysis of polyhistidine-tagged recombinant Adenovirus-2 protein, Strong
Date published: 2017-01-03
Rated 5 out of 5 by from Used for ChIP with human UL3 cellsAdenovirus-2/5 E1A Antibody (M73) Product Citations
Date published: 2016-11-05
Rated 5 out of 5 by from Excellent results with western blotExcellent results with western blot
Date published: 2015-12-02
Rated 3 out of 5 by from ChIP and CoIP using human UL3 cellsChIP and CoIP using human UL3 cells. -SCBT Publication Review
Date published: 2015-07-01
Rated 3 out of 5 by from Used for western blotting with mERas cell lineUsed for western blotting with mERas cell line. -SCBT Publication Review
Date published: 2015-03-17
Rated 3 out of 5 by from Published western blot data using transfectedPublished western blot data using transfected NIH 3T3 and HeLa whole cell lysates. -SCBT Publication Review
Date published: 2015-03-06
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Adenovirus-2/5 E1A 抗体 (M73) is rated 4.0 out of 5 by 10.
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