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Adducin α Antibody (A-5) - Western Blotting - Image 355563
Adducin α 抗体 (A-5): sc-133079. 大鼠脑组织提取物中 Adducin α 表达的 Western 印迹分析.
Adducin α 抗体 (A-5): sc-133079. 大鼠脑组织提取物中 Adducin α 表达的 Western 印迹分析.

Adducin α 抗体 (A-5): sc-133079

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说明书
  • Adducin α抗体(A-5)是小鼠单克隆IgG2a κ, 在3篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Adducin α的氨基酸581-680
  • 推荐用于 mouse, rat 和 human 来源的Adducin α WB, IP, IF, IHC(P) 和 ELISA检测
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 Adducin α 抗体 (A-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Adducin α 抗体 (A-5) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

附加Adducin抗体，包括Adducin α, Adducin β和Adducin γ

Adducin是由三个基因（α、β和γ）编码的细胞骨架蛋白家族。 Adducin是与质膜内叶相关的蛋白质，是位于膜骨架的收缩蛋白-肌动蛋白连接处的蛋白质之一。皮质肌动蛋白骨架网络在细胞凋亡过程中丢失，Adducin是皮质肌动蛋白网络组织的核心。 Adducinα是与肾小管钠泵活性相关的细胞骨架蛋白，与高血压有关。与Adducinβ的限制性表达相反，Adducinα和Adducinγ的表达无处不在。 Adducin β在人类的大脑和造血组织（如骨髓）中表达水平较高，而在小鼠脾脏中表达水平较高。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Adducin α 抗体 (A-5) 参考文献:

  1. Adducin 促进支气管上皮细胞侧膜中 beta2-spectrin 的微米级组织。  |  Abdi, KM. and Bennett, V. 2008. Mol Biol Cell. 19: 536-45. PMID: 18003973
  2. γ-Adducin刺激对噻嗪敏感的NaCl共转运体。  |  Dimke, H., et al. 2011. J Am Soc Nephrol. 22: 508-17. PMID: 21164023
  3. Hts/Adducin控制着突触的形成和消除。  |  Pielage, J., et al. 2011. Neuron. 69: 1114-31. PMID: 21435557
  4. 维持轴突直径需要肌动蛋白结合蛋白α-Adducin  |  Leite, SC., et al. 2016. Cell Rep. 15: 490-498. PMID: 27068466
  5. 脂质筏相关的β-adducin参与了中性粒细胞的迁移。  |  Yang, C., et al. 2018. Mol Med Rep. 18: 1353-1360. PMID: 29901076
  6. 肌动蛋白盖层蛋白 Alpha-Adducin 是 T 细胞成本刺激的必要条件  |  Thauland, TJ., et al. 2019. Front Immunol. 10: 2706. PMID: 31824498
  7. 肌动蛋白结合蛋白α-Adducin调节去蛋白体的转换和可塑性  |  Hiermaier, M., et al. 2021. J Invest Dermatol. 141: 1219-1229.e11. PMID: 33098828
  8. 敲除γ-Adducin可促进NG-硝基-L-精氨酸-甲基酯诱导的高血压肾损伤  |  Fan, L., et al. 2021. J Pharmacol Exp Ther. 377: 189-198. PMID: 33414130
  9. 转录后抑制γ-adducin可促进骨肉瘤细胞的增殖和迁移。  |  Suo, Z., et al. 2022. Tumori. 108: 600-608. PMID: 34632867
  10. Adducin 的调控。钙调蛋白结合域的定义以及蛋白激酶 A 和 C 的磷酸化位点。  |  Matsuoka, Y., et al. 1996. J Biol Chem. 271: 25157-66. PMID: 8810272

订购信息

产品名称产品编号规格价格数量收藏夹

Adducin α 抗体 (A-5)

sc-133079
200 µg/ml
RMB2377.00

Adducin α (A-5): m-IgG Fc BP-HRP 套装

sc-539844
200 µg Ab; 10 µg BP
RMB2662.00

Adducin α (A-5): m-IgG2a BP-HRP 套装

sc-546796
200 µg Ab; 10 µg BP
RMB2662.00

I am using Adducin α (A-5): sc-133079 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great Antibody for Western blotRobust, clean signal on western blot using whole cell lysate from human primary cells and breast cancer cell lines. Relative expression levels in different cell lines were quantified with LC-MS/MS, and correlated well with the western blot.
Date published: 2017-11-17
Rated 4 out of 5 by from Excellent western blot resultI used this antibody in western blot to detect recombinant human Adducin a, the result is excellent but have a bit background.
Date published: 2016-12-06
Rated 5 out of 5 by from Band of interest observed in WB in nonBand of interest observed in WB in non-transfected 293T, mouse Adducin transfected 293T and T98G whole cell lysates. -SCBT QC
Date published: 2015-03-22
Rated 5 out of 5 by from Robust positive band obtained in WB in T98GRobust positive band obtained in WB in T98G and H4 whole cell lysates. -SCBT QC
Date published: 2015-02-24
Rated 5 out of 5 by from WB and IF publishable data with human HaCaTWB and IF publishable data with human HaCaT and keratinocytes - SCBT Publication Review
Date published: 2015-02-06
Rated 5 out of 5 by from Beautiful immunofluorescence membrane andBeautiful immunofluorescence membrane and cytoplasmic staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2014-05-07
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Adducin α 抗体 (A-5) is rated 4.8 out of 5 by 6.
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