Date published: 2025-9-6

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Actin 抗体 (NH3): sc-58679

4.6(5)
寫評論提問

说明书
  • Actin抗体(NH3)是小鼠单克隆IgM κ, 在17篇文献中引用,规格为200 µg/ml
  • 针对human来源的单核细胞和U-937组织溶解性淋巴瘤
  • 推荐用于 mouse, rat, human 和 rabbit 来源的Actin WB, IP, IF, IHC(P) 和 FCM检测; 与N-terminal 40 amino acids不交叉反应
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • 看 beta Actin (C4): sc-47778,可查看有标记的 Actin 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成Actin 抗体 (NH3)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

肌动蛋白抗体(NH3)是一种小鼠单克隆 IgM kappa 轻链抗体,设计用于小鼠、大鼠、人类和兔子,其表位针对单核细胞和人类 U-937 组织细胞淋巴瘤。肌动蛋白是真核细胞中最丰富、最基本的蛋白质之一,在维持细胞结构和功能方面起着关键作用。肌动蛋白形成动态丝状结构(F-actin),参与各种关键的细胞过程,如细胞内运输、粘附、迁移、细胞分裂和细胞形状调节。在高等真核生物中,肌动蛋白有多种异构体表达,每种异构体都具有特定的功能:α-肌动蛋白主要存在于肌肉组织中,而β-和γ-肌动蛋白则更常见于非肌肉细胞中。肌动蛋白异构体的多样性使其能够发挥各种作用,包括提供结构支持、调节基因表达和控制细胞信号通路。在单核细胞等免疫细胞中,肌动蛋白在吞噬和免疫反应等过程中发挥着重要作用。U-937 细胞来源于人类组织细胞淋巴瘤,经常被用于研究免疫相关功能,尤其是吞噬细胞分化。了解肌动蛋白在这些细胞中的作用有助于深入了解癌症和免疫紊乱等疾病,因此抗肌动蛋白抗体 (NH3) 成为研究中的重要工具。此外,肌动蛋白的动态受各种肌动蛋白结合蛋白(ABPs)的调控,这些蛋白影响着细胞运动和收缩等过程,进一步强调了肌动蛋白在细胞功能中的重要性。通过使用 Western 印迹 (WB)、免疫沉淀 (IP) 和流式细胞术 (FCM) 等技术,研究人员可以研究细胞结构、细胞运动以及依赖于肌动蛋白动力学的各种细胞过程。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

Actin 抗体 (NH3)

sc-58679
200 µg/ml
RMB2377.00

Actin 抗体 (NH3) FITC

sc-58679 FITC
200 µg/ml
RMB2482.00

Actin 抗体 (NH3) PE

sc-58679 PE
200 µg/ml
RMB2580.00

Can Actin (NH3): sc-58679 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Actin (NH3): sc-58679 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_9, d_6, h_5CST
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Rated 5 out of 5 by from Used this antibody in IFUsing this antibody is IF, images were really good
Date published: 2017-08-11
Rated 5 out of 5 by from Strong and reliable for IHC and WBA great choice if you are looking for an antibody for IHC or WB. Nice dependable loading control antibody.
Date published: 2017-01-20
Rated 5 out of 5 by from Cytoplasmic staining with immunoperoxidase staininFormalin-fixed, paraffin-embedded human skeletal muscle was stained with immunoperoxidase for cytoplasmic staining of myocytes.
Date published: 2017-01-10
Rated 4 out of 5 by from Good localization to the cytoskeleton in methanolGood localization to the cytoskeleton in methanol-fixed HeLa cells. -SCBT QC
Date published: 2014-04-09
Rated 4 out of 5 by from Used 1:100 dilution in Western Blot withUsed 1:100 dilution in Western Blot with amniotic and coelomic cells (human) -SCBT Publication Review
Date published: 2008-01-20
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Actin 抗体 (NH3) is rated 4.6 out of 5 by 5.
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