Date published: 2025-9-7

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ACTH 抗体 (B427): sc-57021

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说明书
  • ACTH 抗体 B427 是小鼠单克隆 IgG1 κ,ACTH抗体, 在12篇文献中引用,规格为200 µg/ml
  • 针对氨基酸1-24,映射到N-terminus,ACTH的human起源
  • 推荐用于 mouse, rat 和 human 来源的POMC and the processed active peptide ACTH WB, IP, IF 和 IHC(P)检测
  • 关于如何获取ACTH (B427): sc-57021免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 ACTH 抗体 (B427) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ACTH 抗体 (B427) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

促肾上腺皮质激素抗体(B427)是一种鼠单克隆IgG1 kappa轻链抗体,专门用于检测促肾上腺皮质激素(ACTH),一种由前体蛋白促黑素细胞激素(POMC)衍生的肽。抗ACTH抗体(B427)针对人ACTH N端1-24位氨基酸,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和石蜡包埋切片免疫组织化学(IHCP)检测小鼠、大鼠和人类样本中的POMC和加工后的活性肽ACTH。ACTH,又称促肾上腺皮质激素,是由垂体前叶在生物应激时产生的39个氨基酸的肽类激素,可刺激肾上腺皮质分泌皮质醇。ACTH的结构至关重要,因为其N端区域(氨基酸1-24)对于与肾上腺细胞上的黑素细胞2受体(MC2R)结合至关重要,从而触发皮质醇释放,而皮质醇对于应激反应、新陈代谢和免疫功能至关重要。了解ACTH的结构与功能关系对于研究库欣氏病、阿狄森氏病和POMC缺乏症等疾病非常重要,这些疾病以肾上腺功能不全、肥胖和色素沉着异常(如红发)为特征。POMC基因突变会破坏ACTH和其他肽(如α-MSH和β-内啡肽)的加工,导致各种内分泌和代谢异常。ACTH 单克隆抗体(B427)为研究人员提供了研究 ACTH 和 POMC 表达、处理和功能的可靠工具,有助于内分泌学和神经生物学的发展。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

ACTH 抗体 (B427)

sc-57021
200 µg/ml
$316.00

ACTH (B427): m-IgG Fc BP-HRP 套装

sc-526892
200 µg Ab; 10 µg BP
$354.00

ACTH (B427): m-IgG1 BP-HRP 套装

sc-532265
200 µg Ab; 20 µg BP
$354.00

I am using ACTH (B427): sc-57021 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-04-21
  • y_2025, m_9, d_6, h_5CST
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Rated 5 out of 5 by from Good choice for IHC(P)Beautiful specific staining on IHC(P) using a dilution of 1:50. Strong and dependable, this antibody is a great addition to anyone researching ACTH.
Date published: 2017-01-18
Rated 4 out of 5 by from Good for ImmunofluorescenceI bought this antibody about 8 months ago, and used it to detect POMC neurons in mouse hypothalamic slices.This antibody worked pretty good with high spectificity in my immunofluorescence experiments.
Date published: 2016-12-28
Rated 5 out of 5 by from Published results of immunoperoxidase stainingPublished results of immunoperoxidase staining using paraffin embedded murine pituitary glands. -SCBT Publication Review
Date published: 2015-03-16
Rated 5 out of 5 by from Clean band in western blot using transfectedClean band in western blot using transfected whole cell lysates. -SCBT QC
Date published: 2015-03-13
Rated 4 out of 5 by from Positive results in western blot analysisPositive results in western blot analysis of ACTH expression in AtT-20/D16vF2 whole cell lysate and rat pituitary tissue extract. -SCBT QC
Date published: 2015-03-03
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ACTH抗体 (B427) is rated 4.6 out of 5 by 5.
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