Date published: 2025-9-7

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Act1 抗体 (WW-18): sc-100647

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说明书
  • Act1抗体(WW-18)是小鼠单克隆IgG2a κ, 在16篇文献中引用,规格为100 µg/ml
  • 免疫human物种的重组Act1
  • 推荐用于 mouse, rat 和 human 来源的Act1 WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取Act1 (WW-18): sc-100647免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG2a BP-HRP 是 Act1 抗体 (WW-18) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Act1 抗体 (WW-18) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Act1抗体(WW-18)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)等方法检测小鼠、大鼠和人类样本中的Act1。Act1,也称为NFκB激活因子1或CIKS(与IKK和SAPK/JNK有关),在调节NFκB和JNK信号通路中起着至关重要的作用,这些信号通路对于免疫和炎症反应以及细胞在压力条件下的存活至关重要。Act1主要位于细胞质中,与IKKγ结合,促进NFκB和JNK通路的激活。Act1的正常运作至关重要,因为Act1是这两种由压力引起的信号传导通路之间的协调者,确保细胞能够有效应对各种刺激。Act1 (WW-18)抗体以非结合形式提供,使其成为研究人员研究细胞信号传导的复杂机制以及Act1在健康和疾病中的作用的多功能工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Act1 抗体 (WW-18) 参考文献:

  1. 磷酸化与泛素化:NF-[kappa]B 活性的控制。  |  Karin, M. and Ben-Neriah, Y. 2000. Annu Rev Immunol. 18: 621-63. PMID: 10837071
  2. Act1 是一种 NF-kappa B 活化蛋白。  |  Li, X., et al. 2000. Proc Natl Acad Sci U S A. 97: 10489-93. PMID: 10962024
  3. CIKS,连接 Ikappa B 激酶和应激活化蛋白激酶。  |  Leonardi, A., et al. 2000. Proc Natl Acad Sci U S A. 97: 10494-9. PMID: 10962033
  4. 适配蛋白 CIKS 在激活 IKK 复合物中的作用。  |  Mauro, C., et al. 2003. Biochem Biophys Res Commun. 309: 84-90. PMID: 12943667
  5. IL-17A 在增强人气道上皮细胞细胞因子表达的过程中需要 JAK 介导的 PI3K 信号和 ACT1/TRAF6/TAK1 依赖性 NF-kappaB 激活。  |  Huang, F., et al. 2007. J Immunol. 179: 6504-13. PMID: 17982039
  6. Act1通过其在CD40L/BAFF和IL-17信号传导中的双重功能调节自身免疫。  |  Li, X. 2008. Cytokine. 41: 105-13. PMID: 18061473
  7. Act1 通过直接抑制 STAT3 成为 T 细胞和 B 细胞的负调控因子。  |  Zhang, CJ., et al. 2018. Nat Commun. 9: 2745. PMID: 30013031
  8. IL-17A通过ACT1依赖性激活YAP-AREG轴促进银屑病相关的角质形成细胞增殖。  |  Yu, Z., et al. 2022. J Invest Dermatol. 142: 2343-2352. PMID: 35304250
  9. 红景天苷通过调节Act1-TRAF6-p38 MAPK通路,抑制IL-17A介导的肺泡上皮细胞铁死亡,从而减轻HALI。  |  Guo, B., et al. 2022. Cell Commun Signal. 20: 183. PMID: 36411467
  10. Act1通过调节抗氧化RNA代谢和氧化还原平衡来驱动耐药性。  |  Hong, L., et al. 2024. J Exp Med. 221: PMID: 38861022
  11. NF-kappa B 的结构, 调节和功能。  |  Siebenlist, U., et al. 1994. Annu Rev Cell Biol. 10: 405-55. PMID: 7888182
  12. 核因子-kappaB:慢性炎症疾病中的关键转录因子。  |  Barnes, PJ. and Karin, M. 1997. N Engl J Med. 336: 1066-71. PMID: 9091804

订购信息

产品名称产品编号规格价格数量收藏夹

Act1 抗体 (WW-18)

sc-100647
100 µg/ml
RMB2504.00

Act1 (WW-18): m-IgG Fc BP-HRP 套装

sc-539704
100 µg Ab; 10 µg BP
RMB2662.00

Act1 (WW-18): m-IgG2a BP-HRP 套装

sc-546764
100 µg Ab; 10 µg BP
RMB2662.00

Hi, I bought a act-1 antibody from your company. Do you know the recognition region of this antibody? Thank you

Asked by: Weifeng
Thank you for your question. This antibody was raised against recombinant Act1 of human origin. The exact binding epitope has not been mapped. If you have any further questions, please contact your local distributor, or technical service
Answered by: Tech Service
Date published: 2019-10-31

What is the recommended antigen retrieval for this antibody when doing IHC with paraffin-embedded sections?

Asked by: AbPolly
Thank you for your question. We recommend sodium citrate buffer, pH 6.0, with heat for the antigen retrieval step. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 4
Date published: 2017-01-18
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Rated 5 out of 5 by from Good productWorked very well in western blotting. Tested using HepG2 lysate and showed very linear changes with 2 fold dilutions (20 to 2.5 ug/well)
Date published: 2019-08-08
Rated 5 out of 5 by from good antibodygood antibody and you can get very clear result in your wb.
Date published: 2017-11-21
Rated 5 out of 5 by from OutstandingVery nice western blot results with strong clear signal; also strong IF and IHC staining data
Date published: 2017-02-01
Rated 5 out of 5 by from Excellent!This antibody is great in western blot analysis of Act1 expression in Hep G2 nuclear extract.
Date published: 2017-01-10
Rated 5 out of 5 by from Produce nice HRPProduce nice HRP-DAB staining, Parafin section- Sodium Citrate PH6.0 bioling water 20 min; 4 degree 1:50 overnight; Dako Goat Anti mouse-biotin 1:500 ; then Vector strepavdin-HRP 1:200. Human skin tissue.
Date published: 2015-11-18
Rated 5 out of 5 by from Produced beautiful immunoperoxidase membraneProduced beautiful immunoperoxidase membrane and cytoplasmic staining in formalin-fixed, paraffin-embedded human small Intestine tissue. -SCBT QC
Date published: 2015-05-17
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Act1 抗体 (WW-18) is rated 5.0 out of 5 by 6.
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