Date published: 2025-9-9
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ACSL3 Antibody (H-9) - Western Blotting - Image 360084
ACSL3 抗体 (H-9): sc-166374. TE671 (A), A-431 (B), RT-4 (C), C2C12 (D) 和 C6 (E) 全细胞裂解液中 ACSL3 表达的 Western 印迹分析.
ACSL3 抗体 (H-9): sc-166374. TE671 (A), A-431 (B), RT-4 (C), C2C12 (D) 和 C6 (E) 全细胞裂解液中 ACSL3 表达的 Western 印迹分析.

ACSL3 抗体 (H-9): sc-166374

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说明书
  • ACSL3 抗体 H-9 是小鼠单克隆 IgG2b κ，ACSL3抗体, 在12篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的ACSL3的C-terminus的氨基酸645-672之间
  • ACSL3 抗体 (H-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 ACSL3; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗ACSL3抗体（H-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ACSL3 (H-9): sc-166374的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 ACSL3 抗体 (H-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ACSL3 抗体 (H-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外ACSL抗体，包括ACSL1, ACSL3, ACSL4和ACSL5

ACSL3抗体（H-9）是一种IgG2b κ小鼠单克隆ACSL3抗体，可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类的ACSL3蛋白。ACSL3抗体（H-9）既可以以非共轭的抗ACSL3抗体形式提供，也可以以多种共轭的抗ACSL3抗体形式提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。酰基辅酶A合成酶，也称为长链脂肪酸辅酶A合成酶（ACSL）或棕榈酰辅酶A连接酶，包括ACSL1-6，它们都是定位于线粒体、微粒体或过氧化物酶体的单次跨膜蛋白。ACSL蛋白对于细胞脂质的合成和β-氧化降解非常重要。具体而言，ACSL蛋白催化长链脂肪酸活化为酰基辅酶A，其可被代谢形成CO2、三酰甘油（TAG）、磷脂（PL）和胆固醇酯（CE）。在饱和和不饱和长链脂肪酸中，ACSL3优先选择月桂酸、肉豆蔻酸、花生四烯酸和二十碳五烯酸。ACSL3在各种组织中表达为两种同工型，包括脑、心脏、胎盘、前列腺、骨骼肌、睾丸和胸腺。ACSL4优先选择花生四烯酸，并且在类固醇生成组织中含量丰富。ACSL4可能调节女性生育能力和子宫前列腺素的产生。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ACSL3 抗体 (H-9) 参考文献:

  1. 促肾上腺皮质激素和花生四烯酸对酰基-CoA 合成酶 4 表达的调节，酰基-CoA 合成酶 4 是一种在类固醇生成组织中表达的花生四烯酸偏爱酶。  |  Cho, YY., et al. 2000. Biochem Biophys Res Commun. 274: 741-5. PMID: 10924347
  2. 酰基-CoAs 在肝脏中的功能通道：酰基-CoA 合成酶的潜在作用。  |  Muoio, DM., et al. 2000. Am J Physiol Endocrinol Metab. 279: E1366-73. PMID: 11093925
  3. 丙烯酰-CoA 合成酶 4 缺乏症杂合子小鼠的子宫异常并伴有多囊, 子宫前列腺素积聚和生育能力降低。  |  Cho, YY., et al. 2001. Biochem Biophys Res Commun. 284: 993-7. PMID: 11409893
  4. 人类酰基-CoA 合成酶 4 基因的外显子/内含子组织和转录单元。  |  Minekura, H., et al. 2001. Biochem Biophys Res Commun. 286: 80-6. PMID: 11485311
  5. 人类酰基-CoA 合成酶 3 基因的基因组结构和转录单元。  |  Minekura, H., et al. 2001. Gene. 278: 185-92. PMID: 11707336
  6. 长链酰基-CoA 合成酶会调节脂肪酸进入合成途径还是降解途径吗？  |  Coleman, RA., et al. 2002. J Nutr. 132: 2123-6. PMID: 12163649
  7. 黄芩素通过调节GPX4/ACSL4/ACSL3轴抑制铁死亡，从而改善脑缺血再灌注损伤。  |  Li, M., et al. 2022. Chem Biol Interact. 366: 110137. PMID: 36055377
  8. ACSL3调控透明细胞肾细胞癌的脂滴生物生成和铁蛋白沉积敏感性。  |  Klasson, TD., et al. 2022. Cancer Metab. 10: 14. PMID: 36192773
  9. ACSL3和ACSL4在铁死亡和癌症中发挥不同的作用。  |  Yang, Y., et al. 2022. Cancers (Basel). 14: PMID: 36497375
  10. ANKRD1通过促进TRIM25介导的ACSL3泛素化加重肾缺血再灌注损伤。  |  Han, S., et al. 2024. Clin Transl Med. 14: e70024. PMID: 39285846
  11. 大鼠酰基-CoA 合成酶 3 的分子特征和表达。  |  Fujino, T., et al. 1996. J Biol Chem. 271: 16748-52. PMID: 8663269
  12. 交替翻译引发剂产生了具有异质氨基末端的酰基-CoA 合成酶 3 异构体。  |  Fujino, T., et al. 1997. J Biochem. 122: 212-6. PMID: 9276691

订购信息

产品名称产品编号规格价格数量收藏夹

ACSL3 抗体 (H-9)

sc-166374
200 µg/ml
RMB2377.00

ACSL3 (H-9): m-IgGκ BP-HRP 套装

sc-521581
200 µg Ab, 40 µg BP
RMB2662.00

ACSL3 (H-9): m-IgG2b BP-HRP 套装

sc-548931
200 µg Ab; 10 µg BP
RMB2662.00

ACSL3 抗体 (H-9) AC

sc-166374 AC
500 µg/ml, 25% agarose
RMB3129.00

ACSL3 抗体 (H-9) HRP

sc-166374 HRP
200 µg/ml
RMB2377.00

ACSL3 抗体 (H-9) FITC

sc-166374 FITC
200 µg/ml
RMB2482.00

ACSL3 抗体 (H-9) PE

sc-166374 PE
200 µg/ml
RMB2580.00

ACSL3 抗体 (H-9) Alexa Fluor® 488

sc-166374 AF488
200 µg/ml
RMB2685.00

ACSL3 抗体 (H-9) Alexa Fluor® 546

sc-166374 AF546
200 µg/ml
RMB2685.00

ACSL3 抗体 (H-9) Alexa Fluor® 594

sc-166374 AF594
200 µg/ml
RMB2685.00

ACSL3 抗体 (H-9) Alexa Fluor® 647

sc-166374 AF647
200 µg/ml
RMB2685.00

ACSL3 抗体 (H-9) Alexa Fluor® 680

sc-166374 AF680
200 µg/ml
RMB2685.00

ACSL3 抗体 (H-9) Alexa Fluor® 790

sc-166374 AF790
200 µg/ml
RMB2685.00

ACSL3 (H-9) 中和勝肽

sc-166374 P
100 µg/0.5 ml
RMB511.00

70的marker也会在显影时被强着色，小鼠肾脏组织条带很难显色，几乎没有。1:500稀释

Asked by: smallqqi
感谢您的提问。 Marker显色，可能是封闭不彻底或者抗体工作浓度过高，建议加强封闭，调整抗体工作浓度。如需更多咨询，欢迎您发送邮件至我们的亚洲服务部邮箱：[email protected] Thank you for your question. The Marker shows staining, which may be due to incomplete blocking or a too high working concentration of the antibody. It is recommended to strengthen the blocking and adjust the working concentration of the antibody. For further consultation, you are welcome to send an email to the mailbox of our Asia Service Department: [email protected].
Answered by: Ling Chen
Date published: 2025-03-17

sc-166374具体用什么二抗,能用 goat anti mouse IgG(H+L)hrp吗

Asked by: lhlhlh
感谢您的提问。sc-166374是小鼠单克隆 IgG2b κ，您可以用抗小鼠IgG亚型二抗（例如goat anti mouse IgG(H+L)HRP）。如果在我司购买，m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 ACSL3 Antibody (H-9)应用于WB and IHC(P) 首选应用二抗。谢谢。 Thank you for your question. sc-166374 is a mouse monoclonal IgG2b κ, which you can use with anti-mouse IgG subtype secondary antibody (e.g. goat anti mouse IgG(H+L)HRP).If you buy from us,m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ACSL3 Antibody (H-9) for WB and IHC(P) applications. Thank you.
Answered by: Jiajun Z
Date published: 2023-04-07

What application is the blocking peptide sc-166374 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Nice antibodyAntibody works perfect at dilution 1:1000 on cancer cells
Date published: 2018-11-27
Rated 3 out of 5 by from Western blotting on C2C12 cellsApplication : Western Blotting C2C12 cell lysate diluition: 1:1000 weak signal and some aspecific bands
Date published: 2018-10-05
Rated 5 out of 5 by from good antibodythis is a good antibody. it works well in HCC1806 breast cancer cells using a dilution of 1: 1000.
Date published: 2017-11-03
Rated 4 out of 5 by from good antibodyThis ab shows strong band, yet with high background for wb at 1:500.
Date published: 2017-08-22
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmicProduced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human lung tissue. -SCBT QC
Date published: 2015-03-25
Rated 5 out of 5 by from Strong band of interest observed in WB inStrong band of interest observed in WB in TE671 whole cell lysate -SCBT QC
Date published: 2015-02-15
Rated 5 out of 5 by from Positive result in WB in HeLa and KPositive result in WB in HeLa and K-562 whole cell lysates. -SCBT QC
Date published: 2015-01-04
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ACSL3 抗体 (H-9) is rated 4.4 out of 5 by 7.
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