Date published: 2025-9-8

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Abi-1 抗体 (B-12): sc-271180

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说明书
  • Abi-1抗体(B-12)是小鼠单克隆IgG2b κ, 在1篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Abi-1的内部区域内的氨基酸273-309之间
  • 抗-Abi-1 抗体 (B-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Abi-1; 也和以下物种反应,包括: 和 canine and porcine
  • 抗Abi-1抗体(B-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Abi-1 (B-12): sc-271180免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是Abi-1 Antibody (B-12) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Abi-1 Antibody (B-12) 打包提供(请参阅下面的订购信息)。

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関連項目

Abi-1抗体(B-12)是一种IgG2b κ小鼠单克隆Abi-1抗体,能够通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学(IHC,石蜡切片)和酶联免疫吸附试验(ELISA)检测小鼠、大鼠和人的Abi-1蛋白。Abi-1抗体(B-12)既提供非偶联的抗Abi-1抗体形式,也提供多种偶联形式的抗Abi-1抗体,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®偶联物。Abelson致癌基因最初被鉴定为Abelson小鼠白血病病毒(A-MuLV)的病毒转化成分。Abelson基因(ABL1)编码一种含有SH2结构域的酪氨酸激酶,该激酶在生长因子受体连接后进行有丝分裂信号传导。Abl相互作用蛋白Abi-1和Abi-2是含有SH3结构域的蛋白,它们与Abl的富含脯氨酸的基序结合并激活激酶功能。Abi-1的两种剪接变体广泛表达,在骨髓、脾脏、大脑和睾丸中的表达水平最高。Abi-1和Abi-2被认为对细胞生长和转化有负调控作用,包括通过v-Abl进行的细胞转化。在一些急性髓系白血病(AML)病例中,编码Abi-1的基因ABI1已显示易位并与MLL(混合谱系白血病)基因融合。Abi蛋白还被鉴定为通过调节层形足和丝状伪足中的肌动蛋白聚合来调节细胞运动的介质。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Abi-1 抗体 (B-12) 参考文献:

  1. 果蝇阿贝尔森交互蛋白(dAbi)是阿贝尔森酪氨酸激酶活性的正向调节因子。  |  Juang, JL. and Hoffmann, FM. 1999. Oncogene. 18: 5138-47. PMID: 10498863
  2. Abl互作因子1与sos结合,抑制表皮生长因子和v-Abl诱导的细胞外信号调节激酶的活化。  |  Fan, PD. and Goff, SP. 2000. Mol Cell Biol. 20: 7591-601. PMID: 11003655
  3. Abl 互作蛋白定位于薄壁细胞和丝状细胞顶端的肌动蛋白聚合位点。  |  Stradal, T., et al. 2001. Curr Biol. 11: 891-5. PMID: 11516653
  4. 在过表达不同形式的阿贝尔森互作蛋白 Abi-1 的 3T3 细胞中抑制 v-Abl 转化。  |  Ikeguchi, A., et al. 2001. Oncogene. 20: 4926-34. PMID: 11526477
  5. c-abl 的 src 同源 2 结构域的三维溶液结构。  |  Overduin, M., et al. 1992. Cell. 70: 697-704. PMID: 1505033
  6. 在秀丽隐杆线虫外阴发育过程中,sos-1的激活突变使其Dbl结构域成为表皮生长因子受体通路的关键抑制因子。  |  Proepper, C., et al. 2007. EMBO J. 26: 1397-409. PMID: 17304222
  7. Dysbindin-1、WAVE2和Abi-1形成复合物,调节树突棘的形成。  |  Ito, H., et al. 2010. Mol Psychiatry. 15: 976-86. PMID: 20531346
  8. 基于 NESH/Abi-3 的 WAVE2 复合物在功能上有别于基于 Abi-1 的 WAVE2 复合物。  |  Sekino, S., et al. 2015. Cell Commun Signal. 13: 41. PMID: 26428302
  9. 淋巴肉瘤:病毒诱发的小鼠胸腺依赖性疾病。  |  Abelson, HT. and Rabstein, LS. 1970. Cancer Res. 30: 2213-22. PMID: 4318922
  10. 成纤维细胞和淋巴细胞转化所需的 A-MuLV 蛋白序列。  |  Prywes, R., et al. 1983. Cell. 34: 569-79. PMID: 6193890
  11. Abl-Interactor-1是一种与Abl蛋白羧基末端结合的新型SH3蛋白,可抑制v-abl的转化活性。  |  Shi, Y., et al. 1995. Genes Dev. 9: 2583-97. PMID: 7590237
  12. ABI-1是小鼠Abl-互作因子1的人类同源物,它融合了急性髓性白血病中的MLL基因t(10;11)(p11.2;q23)。  |  Taki, T., et al. 1998. Blood. 92: 1125-30. PMID: 9694699

订购信息

产品名称产品编号规格价格数量收藏夹

Abi-1 抗体 (B-12)

sc-271180
200 µg/ml
RMB2377.00

Abi-1 (B-12): m-IgGκ BP-HRP 套装

sc-521868
200 µg Ab, 40 µg BP
RMB2662.00

Abi-1 (B-12): m-IgG2b BP-HRP 套装

sc-548979
200 µg Ab; 10 µg BP
RMB2662.00

Abi-1 抗体 (B-12) AC

sc-271180 AC
500 µg/ml, 25% agarose
RMB3129.00

Abi-1 抗体 (B-12) HRP

sc-271180 HRP
200 µg/ml
RMB2377.00

Abi-1 抗体 (B-12) FITC

sc-271180 FITC
200 µg/ml
RMB2482.00

Abi-1 抗体 (B-12) PE

sc-271180 PE
200 µg/ml
RMB2580.00

Abi-1 抗体 (B-12) Alexa Fluor® 488

sc-271180 AF488
200 µg/ml
RMB2685.00

Abi-1 抗体 (B-12) Alexa Fluor® 546

sc-271180 AF546
200 µg/ml
RMB2685.00

Abi-1 抗体 (B-12) Alexa Fluor® 594

sc-271180 AF594
200 µg/ml
RMB2685.00

Abi-1 抗体 (B-12) Alexa Fluor® 647

sc-271180 AF647
200 µg/ml
RMB2685.00

Abi-1 抗体 (B-12) Alexa Fluor® 680

sc-271180 AF680
200 µg/ml
RMB2685.00

Abi-1 抗体 (B-12) Alexa Fluor® 790

sc-271180 AF790
200 µg/ml
RMB2685.00

Abi-1 (B-12) 中和勝肽

sc-271180 P
100 µg/0.5 ml
RMB511.00

I mostly see two bands, which are very close to each other. Is this right? Why are there two bands? Do the two bands include both Abi-1 and Abi-2? Or just Abi-1? Thanks

Asked by: WZhou
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

What application is the blocking peptide sc-271180 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great for IHC(P)The Abi-1 Antibody (B-12): sc-271180 provides great staining data when performing IHC with formalin fixed, paraffin-embedded tissue sections, showing cytoplasmic localization.
Date published: 2017-01-26
Rated 4 out of 5 by from Great antibody for western blots of porcineGreat antibody for western blots of porcine brain extract, used 1:500 with odyssey block and Licor antibodies and system.
Date published: 2015-10-26
Rated 5 out of 5 by from Produced superb immunoperoxidase cytoplasmicProduced superb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human brain tissue. -SCBT QC
Date published: 2015-01-06
Rated 5 out of 5 by from Good Western Blot data of AbiGood Western Blot data of Abi-1 expression in HeLa whole cell lysate. -SCBT QC
Date published: 2013-09-25
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Abi-1 抗体 (B-12) is rated 4.8 out of 5 by 4.
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