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AATK Antibody (L-37) - Western Blotting - Image 384549
AATK 抗体 (L-37): sc-100436. HeLa 核提取物 (A) 和 CCRF-CEM (B), SK-N-SH (C), NIH/3T3 (D) 和 C6 (E) 全细胞裂解液中 AATK 表达的 Western 印迹分析.
AATK 抗体 (L-37): sc-100436. HeLa 核提取物 (A) 和 CCRF-CEM (B), SK-N-SH (C), NIH/3T3 (D) 和 C6 (E) 全细胞裂解液中 AATK 表达的 Western 印迹分析.

AATK 抗体 (L-37): sc-100436

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说明书
  • AATK 抗体 L-37 是小鼠单克隆 IgG1 (kappa轻链)，AATK抗体，规格为50 µg/0.5 ml
  • 免疫human物种的重组AATK
  • 推荐用于 mouse, rat 和 human 来源的AATK WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取AATK (L-37): sc-100436的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成AATK 抗体 (L-37)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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    関連項目

    AATK抗体（L-37）是一种鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测鼠、大鼠和人类来源的AATK蛋白。抗AATK抗体（L-37）以非结合形式提供。AATK，即细胞凋亡相关酪氨酸激酶，也称为LMR1（狐猴酪氨酸激酶1）、AATYK、AATYK1或LMTK1，是一种单通道I型膜蛋白，主要存在于大脑中，在神经元分化和细胞凋亡中起着关键作用。AATK的重要性在于其在死亡激活通路中的功能，该通路对于调节神经元凋亡至关重要，而神经元凋亡可以最大限度地减少脑损伤并确保正常的神经发育。AATK在细胞凋亡过程中表达，表明它参与髓系祖细胞的生长停滞。从结构上看，AATK的C端有一个富含脯氨酸的域，N端有一个酪氨酸激酶域，有助于体外激酶活性。值得注意的是，由于可变剪接事件的存在，AATK有三种同源物，从而在细胞过程中具有功能多样性。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    AATK 抗体 (L-37) 参考文献:

    1. 通过体细胞杂交分析和荧光原位杂交，确定人类凋亡相关酪氨酸激酶基因在染色体 17q25.3 上的染色体定位。  |  Seki, N., et al. 1999. J Hum Genet. 44: 141-2. PMID: 10083745
    2. 一种新型激酶 AATYK 能诱导和促进人神经母细胞瘤（SH-SY5Y）细胞系的神经元分化。  |  Raghunath, M., et al. 2000. Brain Res Mol Brain Res. 77: 151-62. PMID: 10837911
    3. 替代剪接的 AATYK mRNA 的特征：AATYK 在大脑和神经细胞中的表达模式。  |  Baker, SJ., et al. 2001. Oncogene. 20: 1015-21. PMID: 11314039
    4. 中枢神经系统中表达的凋亡相关酪氨酸激酶（AATYK）的特征。  |  Tomomura, M., et al. 2001. Oncogene. 20: 1022-32. PMID: 11314040
    5. 凋亡相关酪氨酸激酶（AATYK）在发育中小鼠大脑中的差异表达和功能。  |  Tomomura, M., et al. 2003. Brain Res Mol Brain Res. 112: 103-12. PMID: 12670708
    6. 凋亡相关酪氨酸激酶（AATYK）在小脑颗粒细胞存活和凋亡条件下具有不同的钙离子依赖性磷酸化状态。  |  Tomomura, M. and Furuichi, T. 2005. J Biol Chem. 280: 35157-63. PMID: 16100393
    7. miR-1250-5p 是一种在非霍奇金淋巴瘤中发生高甲基化的新型肿瘤抑制性内含子 miRNA：对 ERK 信号传导和细胞迁移有影响的新靶细胞。  |  Zhang, MY., et al. 2021. Cell Commun Signal. 19: 62. PMID: 34044822
    8. CircPTK2 通过靶向 MiR-196a-3p/AATK 轴抑制胃癌进展  |  Gao, L., et al. 2021. Front Oncol. 11: 706415. PMID: 34604044
    9. 表观遗传沉默的凋亡相关酪氨酸激酶（AATK）会促进细胞周期蛋白 D1 和 WEE1 的表达减少，使 TP53 磷酸化，并以激酶依赖的方式减少细胞增殖。  |  Woods, ML., et al. 2022. Cancer Gene Ther. 29: 1975-1987. PMID: 35902728
    10. 118 个中东家庭智力残疾遗传研究的诊断率、候选基因和陷阱。  |  Al-Kasbi, G., et al. 2022. Sci Rep. 12: 18862. PMID: 36344539
    11. 糖酵解代谢物磷酸烯醇丙酮酸通过促进 AATK 的表达，保护宿主免受病毒感染。  |  Zhao, L., et al. 2023. Eur J Immunol. 53: e2350536. PMID: 37724936
    12. 通过促进吉非替尼耐药肺癌细胞的内质体再循环，下调AATK可提高其对铁中毒的易感性。  |  Lee, WC., et al. 2025. J Pathol.. PMID: 39871626

    订购信息

    产品名称产品编号规格价格数量收藏夹

    AATK 抗体 (L-37)

    sc-100436
    50 µg/0.5 ml
    		$333.00

    why the size of data sheet for this antibody is 145 kDa but the western blot picture showed from 86-110 kDa ? any explanation ?

    Asked by: ody82
    Thank you for your question. According to Uniprot (see below), AATK has 3 isoforms with predicted molecular weights of 145 kDa, 45 kDa and 93 kDa. This antibody would not be able to recognize isoform 2 (missing amino acids 1-433). Based on the observed molecular weights alone, this antibody may recognize both isoform 1 and 3 in the cell lines tested. https://www.uniprot.org/uniprot/Q6ZMQ8#sequences
    Answered by: Technical Support
    Date published: 2019-05-03

    Can sc-100436: AATK (L-37) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Asked by: Professor Griffin
    Thank you for your question. Yes, sc-100436: AATK (L-37) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-03-24
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    Rated 5 out of 5 by from Good for Western BlotAntibody detects AATK by Western blot in Hela nuclear extract and CCRF-CEM, SK-N-SH, NIH/3T3, and C6 whole cell lysates -SCBT QC
    Date published: 2023-09-14
    Rated 4 out of 5 by from Good in WB, questionable species cross reactivityWorks well in untransfected HEK293 lysate, single clean band of the correct size when used at 1:250 in 1% milk. However, nothing in whole brain lysate. LMTK1 is clearly enriched in neurons as per multiple literature citations. Maybe the antibody does not cross react with mouse and only recognizes human protein?
    Date published: 2018-04-13
    Rated 5 out of 5 by from good Ab for human samplei bought this Ab a month ago and i did western blot in human tissues. the signal was great.
    Date published: 2018-04-11
    Rated 4 out of 5 by from Great immunoperoxidase membrane and cytoplasmicGreat immunoperoxidase membrane and cytoplasmic staining in formalin-fixed, paraffin-embedded human stomach tissue. -SCBT QC
    Date published: 2015-03-16
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    AATK 抗体 (L-37) is rated 4.4 out of 5 by 5.
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