Date published: 2025-9-7

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AAK1 抗体 (LW-M11): sc-134242

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说明书
  • AAK1 抗体 LW-M11 是小鼠单克隆 IgG2a κ,AAK1抗体, 在1篇文献中引用,规格为100 µg/ml
  • 针对human重组AAK1蛋白的抗原性氨基酸
  • 推荐用于 human 来源的AAK1 WB, IP 和 ELISA检测
  • 关于如何获取AAK1 (LW-M11): sc-134242免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是AAK1 Antibody (LW-M11) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与AAK1 Antibody (LW-M11) 搭配使用(请参阅下面的订购信息)。

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関連項目

AAK1抗体(LW-M11)是一种IgG2a κ小鼠单克隆AAK1抗体(也称为AAK1抗体),可通过蛋白质印迹、免疫沉淀和酶联免疫吸附试验检测人源AAK1蛋白。AAK1抗体(LW-M11)以非偶联形式的抗AAK1抗体存在。衔接蛋白复合体2(AP-2复合体)在受体介导的内吞作用过程中起作用,启动网格蛋白组装,与膜结合受体结合并动员内吞辅助因子。AAK1(AP2相关激酶1),也称为衔接蛋白相关激酶1,是一种由961个氨基酸组成的蛋白质,属于蛋白激酶超家族和丝氨酸/苏氨酸蛋白激酶家族。AAK1由映射到人类染色体2p14的基因编码,参与ATP和核苷酸结合、蛋白质丝氨酸/苏氨酸激酶功能和转移酶活性。AAK1磷酸化AP-2复合体的一个亚基,这导致AP-2与膜结合受体中的分拣信号结合,从而导致受体内吞。AAK1包含一个蛋白激酶结构域,其激酶活性受网格蛋白刺激,并以两种选择性剪接异构体形式存在。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

AAK1 抗体 (LW-M11) 参考文献:

  1. AAK1 对 AP2 mu 亚基的磷酸化介导了与膜蛋白分选信号的高亲和力结合。  |  Ricotta, D., et al. 2002. J Cell Biol. 156: 791-5. PMID: 11877457
  2. 确定一种缔合作用激酶 AAK1 是凝集素介导的内吞作用的调节因子。  |  Conner, SD. and Schmid, SL. 2002. J Cell Biol. 156: 921-9. PMID: 11877461
  3. Ark1/Prk1 蛋白激酶家族。内吞作用和肌动蛋白骨架的调节器。  |  Smythe, E. and Ayscough, KR. 2003. EMBO Rep. 4: 246-51. PMID: 12634840
  4. 凝集素介导的内吞作用中的激酶  |  Korolchuk, V. and Banting, G. 2003. Biochem Soc Trans. 31: 857-60. PMID: 12887321
  5. AP-2在凝集素介导的内吞过程中的不同需求  |  Conner, SD. and Schmid, SL. 2003. J Cell Biol. 162: 773-9. PMID: 12952931
  6. 丝氨酸/苏氨酸激酶 cyclin G-associated kinase 调节表皮生长因子受体信号传导。  |  Zhang, L., et al. 2004. Proc Natl Acad Sci U S A. 101: 10296-301. PMID: 15240878
  7. 揭开 Na+-K+-ATP 酶内吞的神秘面纱:磷酸化是关键。  |  Collawn, JF. 2006. Am J Respir Cell Mol Biol. 35: 1-2. PMID: 16782842
  8. 一种新型 AAK1 剪接变体在内吞途径的多个步骤中发挥作用。  |  Henderson, DM. and Conner, SD. 2007. Mol Biol Cell. 18: 2698-706. PMID: 17494869
  9. 人类细胞对激酶的需求:II.基因相互作用筛选确定了癌细胞中 HPV16 E7 表达后对激酶的要求。  |  Baldwin, A., et al. 2008. Proc Natl Acad Sci U S A. 105: 16478-83. PMID: 18948598

订购信息

产品名称产品编号规格价格数量收藏夹

AAK1 抗体 (LW-M11)

sc-134242
100 µg/ml
$333.00

AAK1 (LW-M11): m-IgG Fc BP-HRP 套装

sc-551900
100 µg Ab; 10 µg BP
$354.00

Can this antibody detect AAK1 protein in mouse?

Asked by: Nikki
Thank you for your question. Based on our tests with this product, AAK1 Antibody (LW-M11): sc-134242 can only be recommended for detection of AAK1 of human origin by WB, IP and ELISA.
Answered by: Tech Support Europe
Date published: 2024-04-19

For Western Blot, is it recommended to use denatured or non-denatured conditions with AAK1 (LW-M11): sc-134242 antibody?

Asked by: Randy McDonald
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 4 out of 5 by from acceptableWe got clean band with some background for Western Blot experiments.
Date published: 2018-09-11
Rated 5 out of 5 by from good featuresI applied for the samples several days ago and they gave it to me immediately. very good and sensitive.
Date published: 2018-01-31
Rated 5 out of 5 by from Good choice for WBstrong bands in Western blot analysis of U-87 MG and HeLa whole cell lysates and rat kidney tissue extract.
Date published: 2017-02-09
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AAK1 抗体 (LW-M11) is rated 4.5 out of 5 by 4.
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