Date published: 2025-9-8

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A20/TNFAIP3 抗体 (8E8.38): sc-32797

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说明书
  • A20/TNFAIP3 抗体 8E8.38 是小鼠单克隆 IgG1, 在1篇文献中引用,提供 200 µg/ml
  • 与human A20的锌指结构域(对应于氨基酸375-537)结合
  • 推荐用于 human 来源的A20 WB 和 IP检测
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-32797 X, 200 µg/0.1 ml)
  • 看 A20/TNFAIP3 (A-12): sc-166692,可查看有标记的 A20/TNFAIP3 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成A20/TNFAIP3 抗体 (8E8.38)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

A20抗体(8E8.38)是一种小鼠单克隆IgG1抗体,可通过蛋白质印迹(WB)和免疫沉淀(IP)等方法检测人类样本中的A20。A20是一种重要的Cys2/Cys2锌指蛋白,在肿瘤坏死因子(TNF)和白细胞介素1(IL-1)等多种炎症刺激下会上调,并作为核因子κB(NFκB)基因表达的负调控因子。通过抑制NFκB的激活,A20在炎症反应的消退中发挥着重要作用,从而防止细胞过度受损并促进组织稳态。A20的结构尤为重要,因为A20的C端区域包含七个锌指结构域,其中只有四个结构域对体外抑制TNF诱导的NFκB激活是必需的。这种结构特征使A20能够有效地与TRAF2、TRAF6和IkB激酶(IKK)γ等关键信号蛋白相互作用,这些蛋白对于调节细胞存活和凋亡至关重要。此外,A20与一种新型A20结合蛋白TXBP151的相互作用表明,A20通过某种机制发挥抗凋亡作用。鉴于A20在信号转导通路负反馈调节中的作用,A20和相关蛋白有望成为一系列炎症性疾病的潜在治疗靶点。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

A20/TNFAIP3 抗体 (8E8.38)

sc-32797
200 µg/ml
RMB2377.00

A20/TNFAIP3 抗体 (8E8.38) X

sc-32797 X
200 µg/0.1 ml
RMB2377.00

When should the TransCruz reagent sc-32797 X be used instead of the standard form sc-32797?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 1 out of 5 by from no bandsused sc-525408 to detect the primary sc-32797 antibody in western blot of HEK293 cell lysates. it did not show any bands. works well with another antibody against the target from a different source. GAPDH loading was not a problem. Got no response when asked for the secondary that used to detect the bands displayed in their catalog page.
Date published: 2023-04-17
Rated 4 out of 5 by from Works for western blotting with Jurkat wholeWorks for western blotting with Jurkat whole cell lysates. -SCBT QC
Date published: 2015-06-26
Rated 4 out of 5 by from Published IP data with human Raji and 293Published IP data with human Raji and 293 cell lines - SCBT Publication Review
Date published: 2013-06-10
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A20/TNFAIP3 抗体 (8E8.38) is rated 3.0 out of 5 by 3.
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