Date published: 2025-9-11
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8-oxoG DNA Lesion Antibody (483.15) - Immunofluorescence - Image 142935
8-oxoG DNA 病变抗体 (483. 15): sc-130914. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示核定位.
8-oxoG DNA Lesion Antibody (483.15) - Immunofluorescence - Image 142935
8-oxoG DNA 病变抗体 (483. 15): sc-130914. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示核定位.
8-oxoG DNA 病变抗体 (483. 15): sc-130914. 甲醇固定的 HeLa 细胞免疫荧光染色, 显示核定位.

8-oxoG DNA Lesion 抗体 (483.15): sc-130914

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说明书
  • 8-oxoG DNA Lesion 抗体 483.15 是小鼠单克隆 IgM κ，8-oxoG DNA Lesion抗体, 在23篇文献中引用，规格为200 µg/ml
  • 对带有8-oxoG损伤的DNA的
  • 推荐用于 8-oxoG Lesions IF 和 ELISA检测
  • 关于如何获取8-oxoG DNA Lesion (483.15): sc-130914的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成8-oxoG DNA Lesion 抗体 (483.15)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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研究信息

関連項目

8-oxoG DNA损伤抗体（483.15）是一种IgM κ小鼠单克隆8-oxoG DNA损伤抗体（也称为8-oxoG DNA损伤抗体），可检测8-oxoG DNA损伤蛋白。8-oxoG DNA损伤抗体（483.15）为非共轭形式的抗8-oxoG DNA损伤抗体。DNA（脱氧核糖核酸）是所有已知生物和一些病毒的遗传物质。DNA对于信息的长期存储以及生物体的发育和功能至关重要，它由两条长的反平行核苷酸聚合物组成，含有糖骨架和磷酸基团，它们通过酯键连接在一起，形成双螺旋结构。8-oxoG（8-氧鸟嘌呤）是一种参与氧化性DNA损伤的诱变损伤。在DNA复制过程中，8-oxoG能够与腺嘌呤（A）错误配对，对基因组稳定性构成威胁。所有生物体至少表达两种类型的8-oxoG-DNA糖基化酶（OGG），以修复8-oxoG。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

8-oxoG DNA Lesion 抗体 (483.15) 参考文献:

  1. 修复 8-氧鸟嘌呤的多种 DNA 糖基化酶及其潜在的体内功能。  |  Hazra, TK., et al. 2001. Prog Nucleic Acid Res Mol Biol. 68: 193-205. PMID: 11554297
  2. 在 CHO 细胞系中修复 8-氧鸟嘌呤和 Ogg1-incised apurinic 位点。  |  Boiteux, S. and le Page, F. 2001. Prog Nucleic Acid Res Mol Biol. 68: 95-105. PMID: 11554315
  3. 用免疫荧光法检测易产生过量自由基的老龄 OXYS 大鼠肝细胞中的 8-氧代鸟嘌呤 DNA 病变。  |  Kemeleva, EA., et al. 2006. Mutat Res. 599: 88-97. PMID: 16574166
  4. 应对 8-氧鸟嘌呤威胁的不同 DNA 修复策略。  |  Russo, MT., et al. 2007. Mutat Res. 614: 69-76. PMID: 16769088
  5. 8-oxoguanine 转录诱变的分子基础。  |  Damsma, GE. and Cramer, P. 2009. J Biol Chem. 284: 31658-63. PMID: 19758983
  6. 通过酶联免疫吸附法（ELISA）或带电化学检测的高效液相色谱法（HPLC）测量 DNA 氧化损伤 8-氧鸟嘌呤（8-oxoG）。  |  Drake, DM., et al. 2019. Methods Mol Biol. 1965: 313-328. PMID: 31069684
  7. HDAC1 在衰老大脑和阿尔茨海默病中调节 OGG1 启动的氧化 DNA 损伤修复。  |  Pao, PC., et al. 2020. Nat Commun. 11: 2484. PMID: 32424276
  8. 植物端粒中8-oxoG的定量。  |  Castillo-González, C., et al. 2022. Int J Mol Sci. 23: PMID: 35563379
  9. 将氧化DNA损伤8-OxoG与肿瘤发展和进展联系起来。  |  Zhao, Y., et al. 2022. Yi Chuan. 44: 466-477. PMID: 35729095
  10. 8-oxoG 修复系统在肿瘤发生和癌症治疗中的作用。  |  Li, C., et al. 2022. Cells. 11: PMID: 36497058
  11. 核提取物中DNA结合蛋白的单分子分析（SMADNE）。  |  Schaich, MA., et al. 2023. Nucleic Acids Res. 51: e39. PMID: 36861323
  12. 人体细胞中存在两种不同的 8-氧代鸟嘌呤修复酶：它们在防止突变方面的潜在互补作用。  |  Hazra, TK., et al. 1998. Nucleic Acids Res. 26: 5116-22. PMID: 9801308

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产品名称产品编号规格价格数量收藏夹

8-oxoG DNA Lesion 抗体 (483.15)

sc-130914
200 µg/ml
RMB2377.00

I am looking for an antibody that would be usable for flow cytometry quantification of 8-oxogunine DNA adducts -- WOULD THIS WORK? https://www.scbt.com/p/8-oxog-dna-lesion-antibody-483-15

Asked by: aporeminiscence
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-01-12

What is the recommended protocol for IF with this antibody? I tried methanol fixing (your standard guidelines) and IF with 1:50 dilution and standard secondary antibody, unfortunately didn' get any signal.

Asked by: annani
Thank you for your question. Please check out our recommended protocol here: https://www.scbt.com/de/resources/protocols/immunofluorescence-cell-staining If you are still having issues please contact our Technical Service team directly for support.
Answered by: Tech Support Europe
Date published: 2021-01-18

I want to use this antibody to detect 8-oxoG level on extracted mitochondrial DNA from c.elegans by performing dot blot. I'm wondering whether this antibody preferentially react to single stranded DNA and RNA. Do I need to denature my DNA sample?

Asked by: mike16
Thank you for your question. This is an antibody against the DNA lesion only as it will detect the 8-oxoG lesion itself. We recommend this antibody for ELISA and IF, not Western blotting, and no denaturing should be necessary.
Answered by: Tech Service
Date published: 2019-11-04

130914 8-oxoG is a IgM (Kappa light chain) why your company recommend use second ab is Anti mIgGk FITC or PE (516140 or 51614)? Do you have Anti mIGM k Ab? Can I you Anti mIGM ab as a second Ab?

Asked by: Mingz
Thank you for your question. We recommend the mouse IgG binding proteins for most IgM antibodies as they are fine for this use. If you have an anti-IgM secondary antibody, that will be fine to use as well.
Answered by: Tech Service
Date published: 2019-08-07

Does 8-oxoG DNA Lesion Antibody (483.15): sc-130914 react with bovine samples?

Asked by: Trav11
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Technical Support 13
Date published: 2017-01-20

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with 8-oxoG DNA Lesion Antibody (483.15): sc-130914?

Asked by: Trav11
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Service 11
Date published: 2017-01-16
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Rated 5 out of 5 by from good stainingStrong staining in IF, the dyeing effect was very good.
Date published: 2024-06-03
Rated 1 out of 5 by from Inconsistent from lot to lotI purchased a tube of anti-8-oxoG sc-130914 and optimized an immunofluorescence protocol using it in cultured human fibroblasts. The stain looked very good, and the signal was inducible by treatment with H2O2 or KBrO3 and repressible by treatment with Fpg. I used the whole tube for experiments and purchased a new tube, but the new tube performs terribly. It binds to all DNA so non-specifically that the signal is too strong to quantify even when the antibody is used at a dilution of 1 to 10,000 (I was using the first tube at 1 to 50). You might get lucky and get a good lot, but maybe you won't.
Date published: 2021-09-22
Rated 4 out of 5 by from IF LCLsHo utilizzato la 8-oxoG DNA lesion per IF su linfoblasti e si è rivelato un ottimo prodotto!
Date published: 2021-04-30
Rated 1 out of 5 by from No signal in IFI tested this antibody (purchased <2 months ago) on the cells subjected to 5mM, 1mM and 0 mM (negative control) H2O2 for 1 h twice: (1) using the standard methanol fixing procedure (without permeabilisaition) with buffers containing Triton X-100 0.3%; and (2) following the recommendation of SC Tech Support (https://www.scbt.com/de/resources/protocols/immunofluorescence-cell-staining) with the use of BSA as a blocking agent (the host of my secondary antibody routinely used in the lab is rabbit). In both experiments there's no signal above the 'no antibody' control. I am not an expert in this field but I have read that DNA denaturation is one of the steps of 5-mC IF procedure. Isn't it necessary also for detecting 8-oxoG?
Date published: 2021-01-22
Rated 3 out of 5 by from 8-oxo-G antibody for immunostaining8-oxo-G antibody works for immunostaining with human and porcine cultured cells that were fixed with methanol.
Date published: 2019-09-08
Rated 1 out of 5 by from RetinaI tried this antibody for mouse retina. it is not detecting for me
Date published: 2018-03-14
Rated 5 out of 5 by from GoodStrong staining in IF in HeLa cells, the dyeing effect was very good.
Date published: 2017-04-10
Rated 5 out of 5 by from Great IF StainingSuperb IF staining data; strong signal with low background
Date published: 2017-01-20
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8-oxoG DNA Lesion 抗体 (483.15) is rated 3.6 out of 5 by 11.
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