Date published: 2025-9-11
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8-OHdG Antibody (E-8) - Immunofluorescence - Image 298532
8-OHdG 抗体 (E-8): sc-393871. 福尔马林固定, 石蜡包埋的红鲷鱼肝组织的免疫荧光染色显示肝细胞中的 8-OHdG 染色. 德克萨斯大学海洋科学研究所 Saydur Rahman 博士友情提供.
8-OHdG Antibody (E-8) - Immunofluorescence - Image 298532
8-OHdG 抗体 (E-8): sc-393871. 福尔马林固定, 石蜡包埋的红鲷鱼肝组织的免疫荧光染色显示肝细胞中的 8-OHdG 染色. 德克萨斯大学海洋科学研究所 Saydur Rahman 博士友情提供.
8-OHdG 抗体 (E-8): sc-393871. 福尔马林固定, 石蜡包埋的红鲷鱼肝组织的免疫荧光染色显示肝细胞中的 8-OHdG 染色. 德克萨斯大学海洋科学研究所 Saydur Rahman 博士友情提供.

8-OHdG 抗体 (E-8): sc-393871

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说明书
  • 8-OHdG 抗体 E-8 是小鼠单克隆 IgM κ，8-OHdG抗体, 在77篇文献中引用，规格为200 µg/ml
  • 针对8-羟基-2′-脱氧鸟苷（8-OHdG）-BCP结合物的synthetic来源
  • 推荐用于 8-OHdG (8-Hydroxy-2′-deoxyguanosine) IF, IHC(P) 和 ELISA检测
  • 还可偶联琼脂糖 用于IP; 偶联HRP的产品, 应用于HC(P) and ELISA; 偶联藻红蛋白, FITC, Alexa Fluor® 488或Alexa Fluor® 647的产品, 应用于IF和IHC(P)
  • 关于如何获取8-OHdG (E-8): sc-393871的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成8-OHdG 抗体 (E-8)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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研究信息

関連項目

8-OHdG抗体（E-8）是一种IgM κ型小鼠单克隆8-OHdG抗体（也称为8-羟基鸟嘌呤抗体），通过IF, IHC(P) 和 ELISA检测8-OHdG蛋白。8-OHdG抗体（E-8）既可作为非偶联的抗8-OHdG抗体形式提供，也可作为多种偶联形式的抗8-OHdG抗体提供，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。DNA或RNA损伤会阻碍细胞执行其功能，并可能显著增加肿瘤形成的可能性。DNA和RNA损伤的原因之一是碱基的氧化。8-羟基-2'-脱氧鸟苷、8-羟基鸟嘌呤（8-OHdG）和8-羟基鸟苷酸都是RNA和DNA氧化损伤的标志物。8-羟基-2'-脱氧鸟苷是由活性氧和氮物种产生的，包括羟基自由基和过氧亚硝酸盐。8-羟基鸟嘌呤是参与突变的主要碱基损伤之一，由电离辐射和放射模拟剂引起。8-羟基鸟苷酸在DNA中诱导G到T的颠换，这可能是致突变的。当研究这种类型损伤的影响时，DNA和RNA损伤的标志物是有用的研究工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

8-OHdG 抗体 (E-8) 参考文献:

  1. 8OHdG不是亨廷顿病状态或进展的生物标志物。  |  Borowsky, B., et al. 2013. Neurology. 80: 1934-41. PMID: 23616162
  2. 基于细胞的 8-OHdG 损伤自动定量。  |  Debelec-Butuner, B., et al. 2016. Methods Mol Biol. 1516: 299-308. PMID: 27044043
  3. 假性剥脱性青光眼患者血浆中8-羟基-2'-脱氧鸟苷（8-OHdG）水平升高。  |  Kondkar, AA., et al. 2019. J Ophthalmol. 2019: 8319563. PMID: 31341657
  4. 电镀行业附近居民体内镉和8-羟基-2'-脱氧鸟苷（8-OHdG）水平升高。  |  Xu, P., et al. 2021. Environ Sci Pollut Res Int. 28: 34427-34435. PMID: 33651290
  5. 8-羟基-2'-脱氧鸟苷（8-OHdG）作为职业接触纳米材料引起的DNA氧化损伤的生物标志物：系统综述。  |  Omari Shekaftik, S. and Nasirzadeh, N. 2021. Nanotoxicology. 15: 850-864. PMID: 34171202
  6. 尿液中氧化应激和 DNA 损伤生物标志物 8-hydroxy-2'-deoxyguanosine (8-OHdG) 的快速提取和分析：在孕妇研究中的应用。  |  Bláhová, L., et al. 2023. Int J Hyg Environ Health. 250: 114175. PMID: 37105016
  7. 精神分裂症患者的饮食总抗氧化能力和血清8-OHdG。  |  Karagöl, G., et al. 2023. Int J Psychiatry Med. 58: 544-558. PMID: 37210638
  8. 通过涉及TAC、8-OHdG和Sortilin的生物标志物组合，增强乳头状甲状腺癌的血管浸润评估。  |  Buczyńska, A., et al. 2024. J Clin Endocrinol Metab. 109: 1866-1872. PMID: 38181425
  9. 口香糖相关口腔粘膜下纤维化中唾液、血清和尿液中8-OHdG的比较评估。  |  Prajapati, RK., et al. 2024. J Oral Maxillofac Pathol. 28: 37-41. PMID: 38800434
  10. 患有或未患有妇科癌症的女性尿液中8-羟基-2'-脱氧鸟苷（8-OHdG）水平。  |  Yamamoto, T., et al. 1996. J Obstet Gynaecol Res. 22: 359-63. PMID: 8870419

订购信息

产品名称产品编号规格价格数量收藏夹

8-OHdG 抗体 (E-8)

sc-393871
200 µg/ml
RMB2377.00

8-OHdG 抗体 (E-8) AC

sc-393871 AC
500 µg/ml, 25% agarose
RMB3129.00

8-OHdG 抗体 (E-8) Alexa Fluor® 488

sc-393871 AF488
200 µg/ml
RMB2685.00

8-OHdG 抗体 (E-8) Alexa Fluor® 647

sc-393871 AF647
200 µg/ml
RMB2685.00

8-OHdG 抗体 (E-8) FITC

sc-393871 FITC
200 µg/ml
RMB2482.00

8-OHdG 抗体 (E-8) HRP

sc-393871 HRP
200 µg/ml
RMB2377.00

8-OHdG 抗体 (E-8) PE

sc-393871 PE
200 µg/ml
RMB2580.00

We want to try the antibody 8-OHdG (E-8): sc-393871 for immunofluorescence in human skin in vitro. It is mandatory to permeabilize the sample before adding the antibody? If so, what protocol is suitable for that?

Asked by: Judit
Thank you for your question. No epitope mapping has been performed for this antibody, so whether it targets the target protein's intra- or extracellular domain is not known at this point. You can find our recommended protocol, including instructions for permeabilization and antigen retrieval, under this link: https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: Tech Support Europe
Date published: 2024-05-03

Hi, is it necessary to do antigen retrieval before doing immunofluorescence staining of 8-OHdG Antibody (E-8): sc-393871 if I fixed the sample by 4% PFA, and do the cryosection after OCT embedding?

Asked by: James
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2024-01-25

Hi, since the antibody is IgM kappa, which of the secondary antibody (coupled to HRP) you would recommend for detection on Western blot? Thank you!

Asked by: Researcher_2022
Thank you for your question. Santa Cruz Biotechnology does not offer IgM secondary antibodies .
Answered by: BlakeJ
Date published: 2022-11-14

Hello. I have mouse brain sections made in vibratome and my question is, Which is the ideal antibody to detect 8-OHdG in this kind of samples? Can i use this one?

Asked by: Marc111
Thank you for your question. sc-393871 would be suited for your research. It is recommended for immuno histo chemistry and not restricted by sample origin.
Answered by: Tech Support Europe
Date published: 2022-05-12

What counter-stain can be used for sc-393871? Can I get a 10ug sample for testing my samples before putting a bulk order?

Asked by: bodhi
Thank you for the question and your interest in our products. We will contact you by email regarding your request.
Answered by: Technical Support Europe
Date published: 2018-02-02

What is the best antigen retrieval method for 8-OHdG (E-8)? Does this antibody require high concentration of tween/triton in the blocking serum? Thanks

Asked by: MelMart
Thank you for your question. We recommend using 10 mM sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval when using this antibody, 8-OHdG (E-8): sc-393871, on formalin-fixed paraffin-embedded tissue sections. A link to our recommended protocol is below as a reference. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2023-09-21

Is this 8-OHdG (E-8): sc-393871 monoclonal antibody available directly conjugated to HRP or fluorescent dyes?

Asked by: TinTin
Yes, sc-133225 is available conjugated to agarose (sc-393871 AC), HRP (sc-393871 HRP), phycoerythrin (sc-393871 PE), fluorescein (sc-393871 FITC), Alexa Fluor® 488 (sc-393871 AF488) and Alexa Fluor® 647 (sc-393871 AF647).
Answered by: Technical Support 8
Date published: 2017-02-07
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Rated 5 out of 5 by from HRP tagged antibody works really wellWe used this antibody (1:100) on pre-weaning rat testis and were able to detect 8-OHdG in specific cells in the seminiferous tubules.
Date published: 2021-03-16
Rated 1 out of 5 by from No immunofluorescence stainingNo immunofluorescence 8-OHdG staining in formalin-fixed, paraffin-embedded retinal pigment epithelium of retinal rat new born
Date published: 2020-05-07
Rated 4 out of 5 by from 免疫组化效果还不错大鼠心肌组织，石蜡切片，采用8-OHdG 抗体 (E-8)，结合生物素化的二抗，再以亲和素偶联的HRP孵育，采用DAB显色，效果挺不错的，可以很好的反应组织内氧化应激水平。
Date published: 2018-02-13
Rated 5 out of 5 by from Excelente resultado!El envió fur muy rápido y el producto es de gran calidad. Totalmente recomendable!
Date published: 2017-11-13
Rated 4 out of 5 by from 8-OHDGThis antibody is working well for the western blot
Date published: 2017-10-30
Rated 5 out of 5 by from great antibodyI use it for IHC and fluorescence. and get very good result.
Date published: 2017-08-18
Rated 5 out of 5 by from 很好组化效果不错，组化可以选择sc-516102二抗，效果不错，比选择组化二抗试剂盒效果好很多，再做荧光看看效果
Date published: 2017-07-06
Rated 5 out of 5 by from good result.good working antibody for WB, this antibody conceration can be used 1:400-1:800,all good result.
Date published: 2017-06-11
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8-OHdG 抗体 (E-8) is rated 4.4 out of 5 by 12.
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