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4E-BP1 Antibody (D-10) - Western Blotting - Image 314551
4E-BP1 抗体 (D-10): sc-514073. HeLa 核提取物 (A), K-562 (B), A-431 (C), HL-60 (D) 和 Hep G2 (E) 全细胞裂解液中 4E-BP1 表达的 Western 印迹分析.
4E-BP1 抗体 (D-10): sc-514073. HeLa 核提取物 (A), K-562 (B), A-431 (C), HL-60 (D) 和 Hep G2 (E) 全细胞裂解液中 4E-BP1 表达的 Western 印迹分析.

4E-BP1 抗体 (D-10): sc-514073

5.0(3)
寫評論提問

说明书
  • 4E-BP1抗体(D-10)是小鼠单克隆IgG2a κ, 在3篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的4E-BP1的N-terminus的氨基酸2-25之间
  • 推荐用于 mouse, rat 和 human 来源的4E-BP1 WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 4E-BP1 (P-1): sc-9977，可查看有标记的 4E-BP1 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 4E-BP1 抗体 (D-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与4E-BP1 抗体 (D-10) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

此外 4E-BP 抗体包括 4E-BP1, 4E-BP2 和 4E-BP3

真核mRNA的蛋白质翻译由多亚基复合物eIF-4F启动，该复合物与mRNA 5'帽结构结合。eIF-4F的组分之一eIF-4E负责结合5'帽结构并组装eIF-4F复合物。调节蛋白4E-BP1（也称为PHAS-I）抑制eIF-4E的功能。S6激酶p70、MAP激酶或PKCs对4E-BP1的磷酸化导致4E-BP1与eIF-4E的解离，促进翻译。与4E-BP1功能相关的蛋白质称为4E-BP2，也与eIF-4E结合。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

4E-BP1 抗体 (D-10)

sc-514073
200 µg/ml
RMB2377.00

4E-BP1 (D-10): m-IgG Fc BP-HRP 套装

sc-538581
200 µg Ab; 10 µg BP
RMB2662.00

4E-BP1 (D-10): m-IgGκ BP-HRP 套装

sc-536219
200 µg Ab; 40 µg BP
RMB2662.00

4E-BP1 (D-10) 中和勝肽

sc-514073 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-514073 P appropriate for?

Asked by: Cweed
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Western blot works great at 1:500 dilutionWestern blot works great at 1:500 dilution, giving a very specific signal at the right size of 4EBP1.
Date published: 2015-10-05
Rated 5 out of 5 by from Good Western Blot data of 4EGood Western Blot data of 4E-BP1 expression in non-transfected and human 4E-BP1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-02-20
Rated 5 out of 5 by from Good Western Blot data of 4EGood Western Blot data of 4E-BP1 expression in HeLa nuclear extract and K-562, A-431, HL-60 and Hep G2 whole cell lysates. -SCBT QC
Date published: 2013-12-28
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4E-BP1 抗体 (D-10) is rated 5.0 out of 5 by 3.
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