TLC Conditions: SiO2; 10% EDTA treated, pH = 9 Alugram Plate; Dichloromethane: Methanol: Water = 4: 4: 1; Visualized with UV and AMCS; Rf = 0.5Identification (1H-NMR) Conditions: DMSO-d6Impurities: Contains 3% Demeclocycline (from epimerization in solution) and 17% unidentified salts. The longer the compound is in solution the greater epimerization to Demeclocycline.