Date published: 2025-9-8
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20S Proteasome β2 Antibody (MCP165) - Western Blotting - Image 126549
20S 蛋白酶体 β2 (MCP165): sc-58410. 未转染 293T: sc-117752 (A), 转染 293T: sc-175561 (B) 和 HeLa (C) 全细胞裂解液中 20S 蛋白酶体 β2 表达的 Western 印迹分析.
20S Proteasome β2 Antibody (MCP165) - Western Blotting - Image 126549
20S 蛋白酶体 β2 (MCP165): sc-58410. 未转染 293T: sc-117752 (A), 转染 293T: sc-175561 (B) 和 HeLa (C) 全细胞裂解液中 20S 蛋白酶体 β2 表达的 Western 印迹分析.
20S Proteasome β2 Antibody (MCP165) - Western Blotting - Image 58344
20S 蛋白酶体 β2 (MCP165): sc-58410. 对 HeLa 全细胞裂解液中 20S 蛋白酶体 β2 的表达进行 Western 印迹分析.
20S 蛋白酶体 β2 (MCP165): sc-58410. 未转染 293T: sc-117752 (A), 转染 293T: sc-175561 (B) 和 HeLa (C) 全细胞裂解液中 20S 蛋白酶体 β2 表达的 Western 印迹分析.

20S Proteasome β2 抗体 (MCP165): sc-58410

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说明书
  • 20S Proteasome beta 2 抗体 MCP165 是小鼠单克隆 IgG1, 在14篇文献中引用，提供 50 µg/0.5 ml
  • 针对human来源的改良红细胞衍生蛋白酶体
  • 推荐用于 mouse, rat 和 human 来源的20S Proteasome β2 WB, IP 和 IF检测
  • 关于如何获取20S Proteasome β2 (MCP165): sc-58410的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是20S Proteasome β2 Antibody (MCP165) for WB applications. 的首选辅助检测试剂，这些试剂现与20S Proteasome β2 Antibody (MCP165) 打包提供（请参阅下面的订购信息）。

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関連項目

附加20S Proteasome抗体，包括20S Proteasome α和20S Proteasome β

20S 蛋白酶体 β2抗体（MCP165）是一种小鼠单克隆 IgG1 抗体，可通过免疫印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）等应用检测小鼠、大鼠和人类样本中的 20S 蛋白酶体 β2（PSMB2）。抗 PSMB2 抗体 (MCP165) 以非结合形式提供，可用于多种实验设置。PSMB2 通过降解不必要的或受损的蛋白质，在细胞蛋白质平衡中发挥着关键作用，这一过程对维持细胞功能和调节各种生物通路至关重要。PSMB2 的结构尤为重要，它由一个圆柱形核心组成，核心由四个堆叠的环构成，每个环包含七个亚基。外环由 α 亚基组成，作为结构元件和调控颗粒的对接位点，内环由 β 亚基组成，具有降解蛋白质所必需的催化活性。这种独特的结构有利于选择性地进入底物，并确保严格调控的降解，防止失控的蛋白水解导致细胞功能障碍。PSMB2 (MCP165) 抗体可帮助研究人员深入了解蛋白酶体的活性及其在癌症和神经退行性疾病等多种疾病中的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

20S Proteasome β2 抗体 (MCP165) 参考文献:

  1. 真核生物20S蛋白酶体催化亚基前肽通过N端乙酰化防止活性位点失活，并促进颗粒组装。  |  Arendt, CS. and Hochstrasser, M. 1999. EMBO J. 18: 3575-85. PMID: 10393174
  2. 水稻（Oryza sativa）20S 蛋白酶体亚基的主要结构特征。  |  Sassa, H., et al. 2000. Gene. 250: 61-6. PMID: 10854779
  3. 4-hydroxynonenal 对 20S 蛋白酶体催化位点的特异性抑制。  |  Ferrington, DA. and Kapphahn, RJ. 2004. FEBS Lett. 578: 217-23. PMID: 15589823
  4. 利用乙烯基砜和 2-酮基-1,3,4-恶二唑优化与人类 20S 蛋白酶体 beta5 亚基的亚位结合：强效选择性蛋白酶体抑制剂的合成与细胞特性。  |  Rydzewski, RM., et al. 2006. J Med Chem. 49: 2953-68. PMID: 16686537
  5. 多发性硬化症患者大脑白质和灰质中20S蛋白酶体的活性降低。  |  Zheng, J. and Bizzozero, OA. 2011. J Neurochem. 117: 143-53. PMID: 21235577
  6. 泛素-蛋白酶体系统在CCL5/RANTES诱导的肌肉萎缩样表型中的作用。  |  Conejeros-Lillo, S., et al. 2024. Eur J Transl Myol. 34: PMID: 38357936
  7. 牛 20S 蛋白酶体结晶的有序结构。  |  Morimoto, Y., et al. 1995. J Biochem. 117: 471-4. PMID: 7629009
  8. 20S 蛋白酶体降解蛋白质过程中的构象限制。  |  Wenzel, T. and Baumeister, W. 1995. Nat Struct Biol. 2: 199-204. PMID: 7773788
  9. 20S 蛋白酶体的结构和结构形成。  |  Schmidt, M., et al. 1997. Mol Biol Rep. 24: 103-12. PMID: 9228290
  10. 通过部分测序从二维凝胶中鉴定的人类蛋白酶体亚基。  |  Kristensen, P., et al. 1994. Biochem Biophys Res Commun. 205: 1785-9. PMID: 7811265
  11. 对 20S 蛋白酶体复合物的全面质谱分析。  |  Huang, L. and Burlingame, AL. 2005. Methods Enzymol. 405: 187-236. PMID: 16413316
  12. 超嗜热古生物暴怒嗜热菌中的 20S 蛋白酶体 beta1 亚基在其功能和稳定性中的作用。  |  Madding, LS., et al. 2007. J Bacteriol. 189: 583-90. PMID: 17114253

订购信息

产品名称产品编号规格价格数量收藏夹

20S Proteasome β2 抗体 (MCP165)

sc-58410
50 µg/0.5 ml
RMB2377.00

20S Proteasome β2 (MCP165): m-IgG Fc BP-HRP 套装

sc-539080
50 µg Ab; 10 µg BP
RMB2662.00

20S Proteasome β2 (MCP165): m-IgG1 BP-HRP 套装

sc-541246
50 µg Ab; 20 µg BP
RMB2662.00

I am using 20S Proteasome β2 (MCP165): sc-58410 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Cweed
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 3 out of 5 by from misleading labelingThis antibody does not detect the Beta 2 subunit of the 20S proteasome as advertised. It was made against PSMB2, which is NOT beta 2. PSMB2 is actually the Beta 4 subunit of the 20S proteasome. Most of Santa Cruz 20S proteasome antibodies are miss-labeled, so be sure to check the target gene and not just the label on the antibody before purchasing. Having said that, this antibody does label nicely (for Beta 4) on western blot.
Date published: 2021-06-29
Rated 5 out of 5 by from It detects what it supposed to.It's an antibody against the 20S Proteasome Beta2 subunit and this is what it detects.
Date published: 2017-04-20
Rated 5 out of 5 by from Strong antibody for WBGreat antibody for 20S Proteasome beta 2 detection, reliable and strong. Good choice!
Date published: 2017-02-08
Rated 3 out of 5 by from Robust band seen in WB using HeLa whole cellRobust band seen in WB using HeLa whole cell lysate. -SCBT QC
Date published: 2015-05-03
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB -SCBT QC
Date published: 2013-08-30
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20S Proteasome β2 抗体 (MCP165) is rated 4.0 out of 5 by 5.
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