Date published: 2025-9-7
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14-3-3 ζ Antibody (1B3) - Western Blotting - Image 414187
14-3-3 ζ 抗体（1B3）：sc-293415。HeLa (A)、K-562 (B) 和 HCT-116 (C) 全细胞裂解液中 14-3-3 ζ 表达的 Western 印迹分析。所用检测试剂：m-IgG Fc BP-HRP：sc-525409。
14-3-3 ζ 抗体（1B3）：sc-293415。HeLa (A)、K-562 (B) 和 HCT-116 (C) 全细胞裂解液中 14-3-3 ζ 表达的 Western 印迹分析。所用检测试剂：m-IgG Fc BP-HRP：sc-525409。

14-3-3 ζ 抗体 (1B3): sc-293415

4.8(6)
寫評論提問

说明书
  • 14-3-3 zeta 抗体 1B3 是小鼠单克隆 IgG1 κ，14-3-3 ζ抗体, 在13篇文献中引用，规格为100 µg/ml
  • 与氨基酸51-150结合，代表human起源的部分长度14-3-3 ζ
  • 推荐用于 mouse, rat 和 human 来源的14-3-3 ζ WB, IP 和 ELISA检测
  • 看 pan 14-3-3 (B-8): sc-133233，可查看有标记的 14-3-3 ζ 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取14-3-3 ζ (1B3): sc-293415的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是14-3-3 ζ Antibody (1B3) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与14-3-3 ζ Antibody (1B3) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外14-3-3抗体，包括14-3-3 β, 14-3-3 ε, 14-3-3 η, 14-3-3 γ, 14-3-3 σ, 14-3-3 θ, 14-3-3 ζ和pan 14-3-3

14-3-3 ζ 抗体 (1B3) 是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过蛋白质印迹 (WB)、免疫沉淀 (IP) 和酶联免疫吸附测定 (ELISA) 检测小鼠、大鼠和人类来源的 14-3-3 ζ 蛋白。抗 14-3-3 ζ 抗体 (1B3) 可作为非结合形式提供。14-3-3 zeta蛋白在细胞信号传导和调节中发挥着关键作用，特别是在癌症生物学中，14-3-3 zeta蛋白参与细胞凋亡、促有丝分裂信号和细胞周期检查点等过程。14-3-3 zeta蛋白主要位于细胞质中，与各种靶蛋白相互作用，调节其活性和稳定性。这种细胞质定位使14-3-3 zeta蛋白能够发挥调节中枢的作用，协调多种信号通路，这对于维持细胞内稳态和应激信号响应至关重要。通过与配体上磷酸化丝氨酸和苏氨酸残基的结合，14-3-3 zeta蛋白能够影响蛋白质的相互作用和功能，从而影响关键的细胞过程。14-3-3 zeta蛋白的表达或功能失调会导致信号异常，并引发包括癌症在内的多种疾病，因此14-3-3 zeta蛋白成为研究和治疗干预的重要目标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

14-3-3 ζ 抗体 (1B3) 参考文献:

  1. DAL-1/蛋白 4.1B 肿瘤抑制因子的 4.1/ezrin/radixin/moesin 结构域与 14-3-3 蛋白相互作用。  |  Yu, T., et al. 2002. Biochem J. 365: 783-9. PMID: 11996670
  2. MADM 是一种新型适配蛋白，可介导髓系白血病因子 1 的 14-3-3 结合位点发生磷酸化。  |  Lim, R., et al. 2002. J Biol Chem. 277: 40997-1008. PMID: 12176995
  3. 14-3-3 癌症联系  |  Hermeking, H. 2003. Nat Rev Cancer. 3: 931-43. PMID: 14737123
  4. 拟南芥中 14-3-3 蛋白的同工酶特异性亚细胞定位似乎是由客户相互作用驱动的。  |  Paul, AL., et al. 2005. Mol Biol Cell. 16: 1735-43. PMID: 15659648
  5. 14-3-3 zeta是癌症治疗的新分子靶标。  |  Matta, A., et al. 2012. Expert Opin Ther Targets. 16: 515-23. PMID: 22512284
  6. 鉴定并确认14-3-3ζ是人参皂苷在脑组织中的新靶点。  |  Chen, F., et al. 2021. J Ginseng Res. 45: 465-472. PMID: 34295206
  7. 发现14-3-3 zeta蛋白是心脏肥大的潜在生物标志物。  |  Mahmud, J., et al. 2023. BMB Rep. 56: 341-346. PMID: 36977605
  8. CSF 14-3-3 zeta(ζ)异构体与阿尔茨海默病的tau病理学和认知能力下降有关。  |  Qiang, Q., et al. 2024. J Neurol Sci. 457: 122861. PMID: 38194803
  9. 14-3-3：信号蛋白的调节剂？  |  Morrison, D. 1994. Science. 266: 56-7. PMID: 7939645
  10. 人类 14-3-3 eta 链基因（YWHAH）的结构组织和染色体分配。  |  Muratake, T., et al. 1996. Genomics. 36: 63-9. PMID: 8812417
  11. 14-3-3 与磷酸肽结合特异性的结构基础。  |  Yaffe, MB., et al. 1997. Cell. 91: 961-71. PMID: 9428519
  12. 一种新型鞘氨醇依赖性蛋白激酶（SDK1）能特异性地使 14-3-3 蛋白的某些同工型发生磷酸化。  |  Megidish, T., et al. 1998. J Biol Chem. 273: 21834-45. PMID: 9705322

订购信息

产品名称产品编号规格价格数量收藏夹

14-3-3 ζ 抗体 (1B3)

sc-293415
100 µg/ml
RMB2377.00

14-3-3 ζ (1B3): m-IgG Fc BP-HRP 套装

sc-537745
100 µg Ab; 10 µg BP
RMB2662.00

14-3-3 ζ (1B3): m-IgGκ BP-HRP 套装

sc-535111
100 µg Ab; 40 µg BP
RMB2662.00

14-3-3 ζ (1B3): m-IgG1 BP-HRP 套装

sc-545335
100 µg Ab; 20 µg BP
RMB2662.00

what is the isoform specificity of this antibody? I am looking for a antibody that identifies only zeta and not the other variants.

Asked by: ussandra
Thank you for your question. This antibody recognizes only 14-3-3 ζ of mouse, rat and human origin by WB, IP and ELISA.
Answered by: Tech Support Europe
Date published: 2024-12-06

Do I need a special antibody diluent for dilution? After dilution, it is mainly used for Western blotting.

Asked by: bob36
Thank you for your question.No special dilutions are needed, you can use PBST or TBST as diluent. Thank you.
Answered by: Jiajun Z
Date published: 2024-10-17

I would like to buy the antibody anti-14-3-3ζ (Santa Cruz Biotechnology, SC-293415) conjugated with FITC. How can I place the order for FITC-conjugated SC-293415? And my second question is whether this antibody is applicable for immunocytochemistry?

Asked by: Surajit
Thank you for your question. 14-3-3 ζ Antibody (1B3): sc-293415 is recommended for detection of 14-3-3 ζ of mouse, rat and human origin by WB, IP and ELISA. It is not recommended for IHC applications. Please contact technical service directlyso we can check whether a FITC conjugate can be offered.
Answered by: Tech Support Europe
Date published: 2021-01-12

I would like to use 14-3-3 ζ (1B3): sc-293415 for immunoprecipitation. Which protein agarose do you recommend using with this antibody?

Asked by: jenniferc
Thank you for your question. We recommend using Protein A/G PLUS-Agarose: sc-2003 https://www.scbt.com/scbt/product/protein-a-g-plus-agarose
Answered by: Technical Support
Date published: 2017-04-13
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Rated 5 out of 5 by from Good for Western BlotAntibody works for Western blot with mouse liver fibroblast cell lysates in published research. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from WBDiluted 1:1000, clear bands were seen in breast cancer cells MCF-7 and MDA-MB-231.
Date published: 2023-06-30
Rated 4 out of 5 by from buon funzionamento per WBAbbiamo provato questo anticorpo per western blot e funziona bene (banda netta).
Date published: 2023-01-27
Rated 5 out of 5 by from WBVery clear band in Western blotting of secretome of C2C12 cells
Date published: 2019-01-25
Rated 5 out of 5 by from Quite good antibody1:500 diluted for rat protein ,the band is strong and special
Date published: 2017-12-30
Rated 5 out of 5 by from Great for Western blotStrong and specific antibody for Western blot, solid single bands seen in HL-60 and human kidney samples.
Date published: 2017-01-31
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14-3-3 ζ 抗体 (1B3) is rated 4.8 out of 5 by 6.
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