Date published: 2025-9-7

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14-3-3 σ 抗体 (5D7): sc-100638

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说明书
  • 14-3-3 σ抗体(5D7)是小鼠单克隆IgG1 κ, 在30篇文献中引用,规格为100 µg/ml
  • 免疫human物种的重组14-3-3 σ
  • 推荐用于 human 来源的14-3-3 σ WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 pan 14-3-3 (B-8): sc-133233,可查看有标记的 14-3-3 σ 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取14-3-3 σ (5D7): sc-100638免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 14-3-3 σ 抗体 (5D7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与14-3-3 σ 抗体 (5D7) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

14-3-3 σ 抗体(5D7)是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)检测人类来源的 14-3-3 σ。抗14-3-3 σ抗体(5D7)以非结合形式提供,可满足各种实验需求。14-3-3 σ,也称为SFN、stratifin、HME1或YWHAS,在细胞信号传导和调节中发挥着关键作用,尤其是在癌症生物学中。14-3-3 σ 主要在分层的鳞状角化上皮中表达,是一种分泌型适配蛋白,可与包括角蛋白17在内的多种靶蛋白相互作用。14-3-3 σ 与角蛋白17的结合可增强Akt/mTOR信号通路,促进蛋白质合成和细胞生长,这是肿瘤发展和进展的重要过程。14-3-3 σ 能够积极调节肿瘤抑制因子 p53,影响细胞周期检查点和细胞凋亡。这种对关键途径的调节凸显了 14-3-3 σ 在维持细胞稳态方面的重要性,以及其在癌症治疗中作为治疗靶点的潜力。14-3-3 σ 单克隆抗体 (5D7) 是癌症生物学及相关领域研究人员的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

14-3-3 σ 抗体 (5D7) 参考文献:

  1. 14-3-3 sigma 可对 p53 进行正向调节,抑制肿瘤生长。  |  Yang, HY., et al. 2003. Mol Cell Biol. 23: 7096-107. PMID: 14517281
  2. 14-3-3sigma 功能特异性的结构基础。  |  Wilker, EW., et al. 2005. J Biol Chem. 280: 18891-8. PMID: 15731107
  3. 14-3-3sigma 是一种 p53 调节因子,可抑制鼻咽癌的肿瘤生长。  |  Yang, H., et al. 2006. Mol Cancer Ther. 5: 253-60. PMID: 16505098
  4. LNCaP 和 Tramp-C1 前列腺癌细胞系中 CpG 岛启动子甲基化和 14-3-3sigma 基因表达沉默与甲基-CpG 结合蛋白 MBD2 有关。  |  Pulukuri, SM. and Rao, JS. 2006. Oncogene. 25: 4559-72. PMID: 16786000
  5. 14-3-3sigma 可控制有丝分裂翻译,促进细胞分裂。  |  Wilker, EW., et al. 2007. Nature. 446: 329-32. PMID: 17361185
  6. 14-3-3 sigma 同工型与角质形成细胞中跨膜 BP180 的细胞质结构域相互作用。  |  Li, Y., et al. 2007. J Cell Physiol. 212: 675-81. PMID: 17443672
  7. 口腔癌中 14-3-3 sigma 的甲基化和肿瘤内异质性。  |  Bhawal, UK., et al. 2007. Oncol Rep. 18: 817-24. PMID: 17786341
  8. 14-3-3sigma 在萌芽期结直肠癌细胞中的过度表达可调节细胞在 tenascin-C 作用下的迁移。  |  Ide, M., et al. 2007. Oncol Rep. 18: 1451-6. PMID: 17982629
  9. 脉络丛肿瘤中 CDH1, RARB 和 SFN 基因的 DNA 甲基化模式。  |  Losi-Guembarovski, R., et al. 2007. Cancer Genet Cytogenet. 179: 140-5. PMID: 18036402
  10. 利用血液样本检测 14-3-3 sigma (σ) 启动子甲基化作为诊断乳腺癌的无创生物标记物。  |  Ye, M., et al. 2017. Oncotarget. 8: 9230-9242. PMID: 27999208
  11. 癌症中的 14-3-3σ 及其调节剂  |  Aljabal, G. and Yap, BK. 2020. Pharmaceuticals (Basel). 13: PMID: 33287252
  12. 14-3-3σ-NEDD4L 轴促进结直肠癌中 HIF-1α 的泛素化和降解。  |  Liu, S., et al. 2023. Cell Rep. 42: 112870. PMID: 37494179

订购信息

产品名称产品编号规格价格数量收藏夹

14-3-3 σ 抗体 (5D7)

sc-100638
100 µg/ml
RMB2504.00

14-3-3 σ (5D7): m-IgG Fc BP-HRP 套装

sc-539703
100 µg Ab; 10 µg BP
RMB2662.00

14-3-3 σ (5D7): m-IgG1 BP-HRP 套装

sc-541661
100 µg Ab; 20 µg BP
RMB2662.00

Can sc-100638: 14-3-3 σ (5D7) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, sc-100638: 14-3-3 σ (5D7) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Good antibody for WBWorked well for western blotting with MCF7 cell line
Date published: 2020-07-01
Rated 5 out of 5 by from Great Choice for WBcrisp strong and clean band in Western Blot applications. Great choice for detecting 14-3-3 sigma in WB.
Date published: 2017-01-26
Rated 5 out of 5 by from WB: works well with AWB: works well with A-431 whole cell lysate -SCBT QC
Date published: 2015-04-01
Rated 5 out of 5 by from Published IF/IHC data using 5D7 on human SW480Published IF/IHC data using 5D7 on human SW480-ADH and HT29 cells. -SCBT Publication Review
Date published: 2015-02-27
Rated 4 out of 5 by from Clean immunohistochemical stain on paraffinClean immunohistochemical stain on paraffin embedded human tumor tissue sections. -SCBT Publication Review
Date published: 2015-02-26
Rated 5 out of 5 by from Superb immunoperoxidase nuclear and cytoplasmicSuperb immunoperoxidase nuclear and cytoplasmic staining in formalin-fixed, paraffin-embedded human uterine cervix tissue. -SCBT QC
Date published: 2015-02-20
Rated 5 out of 5 by from CleanClean, strong band observed in Western blot analysis of 14-3-3 expression in HeLa nuclear extract. -SCBT QC
Date published: 2015-01-26
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14-3-3 σ 抗体 (5D7) is rated 4.7 out of 5 by 7.
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