YB-1抗体 (59-Q) 推荐用于 mouse, rat 和 human 来源的YB-1 WB, IP, IF 和 ELISA检测

YB-1 抗体 (59-Q): sc-101198

YB-1抗体 (59-Q) is rated 4.7 out of 5 by 9.
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Datasheets

    • YB-1抗体(59-Q)是小鼠单克隆IgG2a (kappa轻链),规格为100 µg/ml
    • 免疫human物种的重组YB-1
    • 推荐用于 mouse, rat 和 human 来源的YB-1 WB, IP, IF 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取YB-1 (59-Q): sc-101198免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

Can you please tell me the exact immunogen peptide for generation of YB-1 Antibody (59-Q): sc-101198? Thank you in advance.

Asked by: Mel12
Thank you for your question. This antibody was raised against a recombinant protein mapping within amino acids 51-140 of YB-1 of human origin. The exact epitope has not been mapped, so no further information is available.
Answered by: Tech Service
Date published: 2020-02-19

What is the total volume provided of YB-1 (59-Q): sc-101198?

Asked by: jenni
Each vial contains 100 µg IgG2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Service 11
Date published: 2017-01-17

What is the recommended fixation method for IF with YB-1 (59-Q): sc-101198 monoclonal antibody?

Asked by: jenni
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 5
Date published: 2017-01-16
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Rated 5 out of 5 by from Great Ab Working good in casein blocking buffer (1:500, 100ug/ml); 40ug of protein loaded. Detected 50kDa (major band) and >30kDa (weak and cell line dependent)
Date published: 2018-01-03
Rated 5 out of 5 by from Excellent Antibody for WB. Work well in WB at 1:1000, Hep-1 cells. Strong and clear band was observed.
Date published: 2017-04-16
Rated 5 out of 5 by from Strong western and IF Very nice antibody for western blot studies; also strong IF staining
Date published: 2017-01-24
Rated 5 out of 5 by from Really strong antibody! Great antibody for IHC and WB! It's reliable and specific, so it's a great choice if you're looking for a YB-1 antibody.
Date published: 2017-01-16
Rated 5 out of 5 by from Good signal with IF and WB I got exceptional immunofluorescence cytoplasmic staining as well as western blot in NIH3T3 cells
Date published: 2016-09-09
Rated 4 out of 5 by from This has worked well in WB This has worked well in WB.
Date published: 2015-10-29
Rated 4 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB. -SCBT QC
Date published: 2015-07-13
Rated 5 out of 5 by from Produced exceptional immunofluorescence nuclear Produced exceptional immunofluorescence nuclear and cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-02-09
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