YAP1抗体 (63.7) 推荐用于 mouse, rat 和 human 来源的YAP WB, IP, IF, IHC(P) 和 ELISA检测

YAP1 抗体 (63.7): sc-101199

YAP1抗体 (63.7) is rated 4.6 out of 5 by 42.
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Datasheets

    • YAP1抗体(63.7)是小鼠单克隆IgG2a κ, 在447篇文献中引用,规格为100 µg/ml
    • 免疫human物种的重组YAP
    • 推荐用于 mouse, rat 和 human 来源的YAP WB, IP, IF, IHC(P) 和 ELISA检测
    • 看 YAP1 (G-6): sc-376830,可查看有标记的 YAP1 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
    • 关于如何获取YAP1 (63.7): sc-101199免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP (小鼠IgG Fc 结合蛋白偶联辣根过氧化物酶(HRP))m-IgGκ BP-HRP (小鼠IgGκ 结合蛋白偶联辣根过氧化物酶(HRP)) 是 YAP1 Antibody (63.7) for WB and IHC(P) 的首选应用二抗。这些试剂现在以套装形式提供YAP1 Antibody (63.7)。 (见下方订购信息).

Hi! I wanted to ask what is each of bands shown in the datasheet for this antibody in WB.

Asked by: Anonymous
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Answered by: Dallas Tech Service
Date published: 2021-04-20

Could you please recommend how to perform a suitable antigen retrieval for this antibody (YAP 63.7) on the Paraffin-embedded section immunofluorescence staining? (which buffer/pH or temperature is favorable?)

Asked by: Praphawi
Thank you for your question. You can find our standard protocol under this link: https://www.scbt.com/resources/protocols/immunoperoxidase-staining The recommended method for antigen retrieval is heat treatment as detailed in the protocol.
Answered by: Technical Support Europe
Date published: 2020-10-23

hi all.. currently using the sc101199 clone for immunohistoichemistry. Can someone suggest a proper pH for epitope retrieval? What buffer is best for the purpose?

Asked by: royd
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval with this antibody. See further details in our protocol online. https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2019-12-24

Does this antibody(YAP sc-101199) recognize TAZ? If so, what is immunogen? Thanks.

Asked by: _park
Thank you for your question. No, there is no significant homology between the sequence used to raise our YAP antibody sc-101199 and the protein TAZ. We have no reason to expect cross-reactivity. This antibody was raised against recombinant YAP of human origin, but the exact immunogen has not been mapped.
Answered by: Tech Service
Date published: 2019-11-11

Immunogen是什么?为什么找不到?

Asked by: cardiodynia
Thank you for your inquiry. YAP Antibody (63.7) was raised against amino acid 53-162 of human origin recombinant YAP. 感谢您的提问。YAP 抗体(63.7)的抗原是人重组YAP的53-162个氨基酸序列。
Answered by: Technical Support 9
Date published: 2019-10-29

What is the binding epitope of the antibody and does it detact also TAZ ?

Asked by: JeromeJ
Thank you for your question. This YAP antibody was raised against recombinant YAP of human origin. The exact epitope has not been mapped, so we do not have further information. Although we have not detected TAZ, it is possible that it could pick it up since there is some sequence homology there, but since we have not epitope mapped the epitope, we can not confirm yes or no.
Answered by: Tech Service
Date published: 2019-10-10

Is this YAP antibody also binding to Phosphorylated YAP (Ser 127) ? or specifically binding to non-phosphorylated YAP

Asked by: diekino
Thank you for your question. This is not a phospho-specific antibody. It detects total YAP protein.
Answered by: Technical Support
Date published: 2020-09-29

May I know if the antigen used to raise this antibody is specific to which isoform of YAP1? Or does it cover a region that is 100% conserved in the 8 isoforms of YAP?

Asked by: Anonymous
Thank you for your question. YAP Antibody (63.7): sc-101199 has been raised against recombinant YAP of human origin (protein accession P46937). The epitope region of this antibody has not yet been mapped, so the exact binding site and target isoforms of the antibody are not known.
Answered by: Tech Support Europe
Date published: 2019-07-11
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Rated 5 out of 5 by from YAP1 in Ventana benchmark ultra Fast setup with 2 new batches. It's 2 times a constant staining with a basic IHC protocol (48min. EDTA and 32 mins. primairy).
Date published: 2021-04-06
Rated 4 out of 5 by from しっかり染まる マウス由来の初代培養細胞、ヒト由来株化細胞の染色に使いましたが、典型例と同様にしっかりと検出できました。
Date published: 2020-11-30
Rated 5 out of 5 by from works well for WB I used this antibody at 1:200 dilution and obtained good results. I detected strong band around 65 kDa that disappeared by YAP siRNA.
Date published: 2020-09-06
Rated 5 out of 5 by from very good. very good antibody. it works well in WB, diluted in 1:500~1:1000.
Date published: 2020-08-07
Rated 4 out of 5 by from Buone prestazioni Ho ricevuto l'anticorpo la settimana scorsa e l'ho subito testato. Il risultato è stato molto soddisfacente.
Date published: 2020-07-01
Rated 4 out of 5 by from Buon anticorpo per WB L'anticorpo funziona molto bene per western blot (caricare 30-40 ug). Per immunofluorescenza necessario bloccaggio con BSA 5% e incubazione o/n.
Date published: 2019-12-06
Rated 4 out of 5 by from 잘 잡힙니다! 이 상품을 샘플로 받아서 WB(hTERT-RPE1), ICC(hTERT-RPE1) 실험을 하고 있는데, 잘 사용하고 있고, 구매하였습니다.
Date published: 2019-10-22
Rated 5 out of 5 by from Worked as described We have used this antibody for both IF (1:50 in PBSA) and western blot (1:100 in skim milk TBST) of human-derived adult stem cells cultured in 3D environments. We were able to detect YAP in both cases and obtained nicely defined western blot bands. The antibody needs to be quite concentrated but the 1mL product size covers for several uses.
Date published: 2019-06-13
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