YAP 抗体 (63.7): sc-101199
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- YAP抗体(63.7)是小鼠单克隆IgG2a κ, 在621篇文献中引用，规格为100 µg/ml
- 免疫human物种的重组YAP
- 推荐用于 mouse, rat 和 human 来源的YAP WB, IP, IF, IHC(P) 和 ELISA检测
- 看 YAP (G-6): sc-376830，可查看有标记的 YAP 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
- 关于如何获取YAP (63.7): sc-101199的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 YAP Antibody (63.7) for WB and IHC(P) 的首选应用二抗。这些试剂现在以套装形式提供YAP Antibody (63.7)。 (见下方订购信息).
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Is this reactive to chicken YAP1? Are any of the YAP1 antibodies reactive to chicken YAP1?
Thank you for your question. YAP1 Antibody (63.7): sc-101199 is only recommended for detection of YAP of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. We have not tested this or any other YAP1 antibody in avian cell or tissue samples.
Date published: 2022-11-30
Hi! I wanted to ask what is each of bands shown in the datasheet for this antibody in WB.
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Date published: 2021-04-20
Could you please recommend how to perform a suitable antigen retrieval for this antibody (YAP 63.7) on the Paraffin-embedded section immunofluorescence staining? (which buffer/pH or temperature is favorable?)
Thank you for your question. You can find our standard protocol under this link:
https://www.scbt.com/resources/protocols/immunoperoxidase-staining
The recommended method for antigen retrieval is heat treatment as detailed in the protocol.
Date published: 2020-10-23
hi all.. currently using the sc101199 clone for immunohistoichemistry. Can someone suggest a proper pH for epitope retrieval? What buffer is best for the purpose?
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval with this antibody. See further details in our protocol online.
https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Date published: 2019-12-24
Does this antibody(YAP sc-101199) recognize TAZ? If so, what is immunogen? Thanks.
Thank you for your question. No, there is no significant homology between the sequence used to raise our YAP antibody sc-101199 and the protein TAZ. We have no reason to expect cross-reactivity. This antibody was raised against recombinant YAP of human origin, but the exact immunogen has not been mapped.
Date published: 2019-11-11
Immunogen是什么？为什么找不到？
Thank you for your inquiry. YAP Antibody (63.7) was raised against amino acid 53-162 of human origin recombinant YAP.
感谢您的提问。YAP 抗体(63.7)的抗原是人重组YAP的53-162个氨基酸序列。
Date published: 2019-10-29
What is the binding epitope of the antibody and does it detact also TAZ ?
Thank you for your question. This YAP antibody was raised against recombinant YAP of human origin. The exact epitope has not been mapped, so we do not have further information. Although we have not detected TAZ, it is possible that it could pick it up since there is some sequence homology there, but since we have not epitope mapped the epitope, we can not confirm yes or no.
Date published: 2019-10-10
Is this YAP antibody also binding to Phosphorylated YAP (Ser 127) ? or specifically binding to non-phosphorylated YAP
Thank you for your question. This is not a phospho-specific antibody. It detects total YAP protein.
Date published: 2020-09-29
Rated 5 out of
5
by
Joel P from
Great antibody.
Works great for immunostaining, flow cytometry and western blotting. We've validated all of these using knockdown experiments.
Date published: 2022-12-02
Rated 5 out of
5
by
Wats from
Great Antibody for IF
We use it for IF (4% PFA fixation recommended), it perfectly works for detecting nuclear or cytoplasmic YAP for most of human cell lines. Recommend it with 100% support!
Date published: 2022-03-28
Rated 5 out of
5
by
liuyan123 from
功能很出色哦
我一个多月前购买了此产品，用着挺好的，达到了我预期的效果，感觉很满意。以后还会继续选用SANTA的抗体，也会向同事推荐。
Date published: 2021-12-29
Rated 5 out of
5
by
eleni from
Great antibody!
It works well in immunofluorescence. 1:100 dilution
Date published: 2021-10-21
Rated 5 out of
5
by
Jason 王 from
功能出色
我上个月购买了此产品，到货很快，实验做的效果很好，条带清晰，数据很满意！下次还会购买santa的产品。
Date published: 2021-07-27
Rated 5 out of
5
by
SvK1 from
YAP1 in Ventana benchmark ultra
Fast setup with 2 new batches.
It's 2 times a constant staining with a basic IHC protocol (48min. EDTA and 32 mins. primairy).
Date published: 2021-04-06
Rated 4 out of
5
by
suttoko from
しっかり染まる
マウス由来の初代培養細胞、ヒト由来株化細胞の染色に使いましたが、典型例と同様にしっかりと検出できました。
Date published: 2020-11-30
Rated 5 out of
5
by
mushroommushmush from
works well for WB
I used this antibody at 1:200 dilution and obtained good results. I detected strong band around 65 kDa that disappeared by YAP siRNA.
Date published: 2020-09-06