YAP1 抗体 (63.7): sc-101199
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- YAP1抗体(63.7)是小鼠单克隆IgG2a κ, 在447篇文献中引用，规格为100 µg/ml
- 免疫human物种的重组YAP
- 推荐用于 mouse, rat 和 human 来源的YAP WB, IP, IF, IHC(P) 和 ELISA检测
- 看 YAP1 (G-6): sc-376830，可查看有标记的 YAP1 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
- 关于如何获取YAP1 (63.7): sc-101199的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- m-IgG Fc BP-HRP (小鼠IgG Fc 结合蛋白偶联辣根过氧化物酶(HRP)) 和 m-IgGκ BP-HRP (小鼠IgGκ 结合蛋白偶联辣根过氧化物酶(HRP)) 是 YAP1 Antibody (63.7) for WB and IHC(P) 的首选应用二抗。这些试剂现在以套装形式提供YAP1 Antibody (63.7)。 (见下方订购信息).
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Hi! I wanted to ask what is each of bands shown in the datasheet for this antibody in WB.
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Date published: 2021-04-20
Could you please recommend how to perform a suitable antigen retrieval for this antibody (YAP 63.7) on the Paraffin-embedded section immunofluorescence staining? (which buffer/pH or temperature is favorable?)
Thank you for your question. You can find our standard protocol under this link:
https://www.scbt.com/resources/protocols/immunoperoxidase-staining
The recommended method for antigen retrieval is heat treatment as detailed in the protocol.
Date published: 2020-10-23
hi all.. currently using the sc101199 clone for immunohistoichemistry. Can someone suggest a proper pH for epitope retrieval? What buffer is best for the purpose?
Thank you for your question. We recommend using sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval with this antibody. See further details in our protocol online.
https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Date published: 2019-12-24
Does this antibody(YAP sc-101199) recognize TAZ? If so, what is immunogen? Thanks.
Thank you for your question. No, there is no significant homology between the sequence used to raise our YAP antibody sc-101199 and the protein TAZ. We have no reason to expect cross-reactivity. This antibody was raised against recombinant YAP of human origin, but the exact immunogen has not been mapped.
Date published: 2019-11-11
Immunogen是什么？为什么找不到？
Thank you for your inquiry. YAP Antibody (63.7) was raised against amino acid 53-162 of human origin recombinant YAP.
感谢您的提问。YAP 抗体(63.7)的抗原是人重组YAP的53-162个氨基酸序列。
Date published: 2019-10-29
What is the binding epitope of the antibody and does it detact also TAZ ?
Thank you for your question. This YAP antibody was raised against recombinant YAP of human origin. The exact epitope has not been mapped, so we do not have further information. Although we have not detected TAZ, it is possible that it could pick it up since there is some sequence homology there, but since we have not epitope mapped the epitope, we can not confirm yes or no.
Date published: 2019-10-10
Is this YAP antibody also binding to Phosphorylated YAP (Ser 127) ? or specifically binding to non-phosphorylated YAP
Thank you for your question. This is not a phospho-specific antibody. It detects total YAP protein.
Date published: 2020-09-29
May I know if the antigen used to raise this antibody is specific to which isoform of YAP1? Or does it cover a region that is 100% conserved in the 8 isoforms of YAP?
Thank you for your question. YAP Antibody (63.7): sc-101199 has been raised against recombinant YAP of human origin (protein accession P46937). The epitope region of this antibody has not yet been mapped, so the exact binding site and target isoforms of the antibody are not known.
Date published: 2019-07-11
Rated 5 out of
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SvK1 from
YAP1 in Ventana benchmark ultra
Fast setup with 2 new batches.
It's 2 times a constant staining with a basic IHC protocol (48min. EDTA and 32 mins. primairy).
Date published: 2021-04-06
Rated 4 out of
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suttoko from
しっかり染まる
マウス由来の初代培養細胞、ヒト由来株化細胞の染色に使いましたが、典型例と同様にしっかりと検出できました。
Date published: 2020-11-30
Rated 5 out of
5
by
mushroommushmush from
works well for WB
I used this antibody at 1:200 dilution and obtained good results. I detected strong band around 65 kDa that disappeared by YAP siRNA.
Date published: 2020-09-06
Rated 5 out of
5
by
Benben from
very good.
very good antibody. it works well in WB, diluted in 1:500~1:1000.
Date published: 2020-08-07
Rated 4 out of
5
by
anna67 from
Buone prestazioni
Ho ricevuto l'anticorpo la settimana scorsa e l'ho subito testato. Il risultato è stato molto soddisfacente.
Date published: 2020-07-01
Rated 4 out of
5
by
Lillo from
Buon anticorpo per WB
L'anticorpo funziona molto bene per western blot (caricare 30-40 ug).
Per immunofluorescenza necessario bloccaggio con BSA 5% e incubazione o/n.
Date published: 2019-12-06
Rated 4 out of
5
by
Jihyeon from
잘 잡힙니다!
이 상품을 샘플로 받아서 WB(hTERT-RPE1), ICC(hTERT-RPE1) 실험을 하고 있는데,
잘 사용하고 있고, 구매하였습니다.
Date published: 2019-10-22
Rated 5 out of
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Anonymous from
Worked as described
We have used this antibody for both IF (1:50 in PBSA) and western blot (1:100 in skim milk TBST) of human-derived adult stem cells cultured in 3D environments. We were able to detect YAP in both cases and obtained nicely defined western blot bands. The antibody needs to be quite concentrated but the 1mL product size covers for several uses.
Date published: 2019-06-13