WNK2 抗体 (46.21): sc-100452

WNK2 Antibody (46.21) is an IgG₁ κ mouse monoclonal WNK2 antibody (also designated WNK2 antibody) suitable for the detection of the WNK2 protein of human origin by IF, IHC(P) and ELISA. WNK2 Antibody (46.21) is available as the non-conjugated anti-WNK2 antibody. The protein kinase superfamily contains over a thousand proteins in 57 subfamilies that all share a catalytic core of 250–300 amino acids organized in two domains. WNK, for "with no lysine (K)", kinases are serine-threonine protein kinases that contain a cysteine residue in place of a lysine residue in a family of proteins that traditionally contain a lysine following a short string of hydrophobic residues. WNK kinases contain a lysine upstream of the traditional position, within a glycine string. This lysine functions as an anchor and orients ATP through interactions with the α and β phosphoryl groups. The catalytic domains of WNK2, WNK3 and WNK4 are 95% homologous to WNK1. The human WNK1 gene encodes a 2,382 amino acid protein that is primarily expressed in heart, kidney, muscle and distal nephron. The human WNK3 gene encodes a protein that is primarily expressed in brain; the human WNK4 gene encodes a 1,243 amino acid protein that is expressed in kidney. Aberrant function of WNK kinases and their associated signaling pathways are implicated in hypertension, increased renal salt reabsorption and impaired K+ and H+ excretion.

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References:

1. WNK1, a novel mammalian serine/threonine protein kinase lacking the catalytic lysine in subdomain II.  |  Xu, B., et al. 2000. J Biol Chem. 275: 16795-801. PMID: 10828064
2. Human hypertension caused by mutations in WNK kinases.  |  Wilson, FH., et al. 2001. Science. 293: 1107-12. PMID: 11498583
3. WNK kinases, a novel protein kinase subfamily in multi-cellular organisms.  |  Veríssimo, F. and Jordan, P. 2001. Oncogene. 20: 5562-9. PMID: 11571656
4. Human hypertension caused by mutations in WNK kinases.  |  Hollenberg, NK. 2002. Curr Hypertens Rep. 4: 267. PMID: 12166403
5. Regulation of WNK1 by an autoinhibitory domain and autophosphorylation.  |  Xu, BE., et al. 2002. J Biol Chem. 277: 48456-62. PMID: 12374799
6. Compilation and characterization of a novel WNK family of protein kinases in Arabiodpsis thaliana with reference to circadian rhythms.  |  Nakamichi, N., et al. 2002. Biosci Biotechnol Biochem. 66: 2429-36. PMID: 12506983
7. WNK kinases regulate thiazide-sensitive Na-Cl cotransport.  |  Yang, CL., et al. 2003. J Clin Invest. 111: 1039-45. PMID: 12671053
8. Association of WNK1 gene polymorphisms and haplotypes with ambulatory blood pressure in the general population.  |  Tobin, MD., et al. 2005. Circulation. 112: 3423-9. PMID: 16301342