USP9 抗体 (5G-02): sc-100628

4.0(4)

- USP9 抗体 5G-02 是小鼠单克隆 IgG₁ κ，USP9抗体, 在4篇文献中引用，规格为100 µg/ml
- 免疫human物种的重组USP9
- 推荐用于 human 来源的USP9 WB, IP, IF 和 ELISA检测
- 关于如何获取USP9 (5G-02): sc-100628的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- m-IgG Fc BP-HRP, m-IgG₁ BP-HRP 和 m-IgGκ BP-HRP 是 USP9 Antibody (5G-02) for WB 的首选应用二抗。这些试剂现在以套装形式提供USP9 Antibody (5G-02)。 (见下方订购信息).

References:

Usp9y (ubiquitin-specific protease 9 gene on the Y) is associated with a functional promoter and encodes an intact open reading frame homologous to Usp9x that is under selective constraint. | Hall, NM., et al. 2003. Mamm Genome. 14: 437-47. PMID: 12925892
Doublecortin interacts with the ubiquitin protease DFFRX, which associates with microtubules in neuronal processes. | Friocourt, G., et al. 2005. Mol Cell Neurosci. 28: 153-64. PMID: 15607950
Spatially and temporally specific expression in mouse hippocampus of Usp9x, a ubiquitin-specific protease involved in synaptic development. | Xu, J., et al. 2005. J Neurosci Res. 80: 47-55. PMID: 15723417
Age-related changes in Usp9x protein expression and DNA methylation in mouse brain. | Xu, J. 2005. Brain Res Mol Brain Res. 140: 17-24. PMID: 16023255
14-3-3 cooperates with LKB1 to regulate the activity and localization of QSK and SIK. | Al-Hakim, AK., et al. 2005. J Cell Sci. 118: 5661-73. PMID: 16306228
The ubiquitin ligase itch is auto-ubiquitylated in vivo and in vitro but is protected from degradation by interacting with the deubiquitylating enzyme FAM/USP9X. | Mouchantaf, R., et al. 2006. J Biol Chem. 281: 38738-47. PMID: 17038327
Control of AMPK-related kinases by USP9X and atypical Lys(29)/Lys(33)-linked polyubiquitin chains. | Al-Hakim, AK., et al. 2008. Biochem J. 411: 249-60. PMID: 18254724
Characterisation of the coding sequence and fine mapping of the human DFFRY gene and comparative expression analysis and mapping to the Sxrb interval of the mouse Y chromosome of the Dffry gene. | Brown, GM., et al. 1998. Hum Mol Genet. 7: 97-107. PMID: 9384609

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	USP9X	Entrez Gene ID: 8239	Xp11.4	XM_005272675, XM_005272676, NM_001039590, NM_001039591, NM_004652, NM_021906	UniProt ID: Q93008	n/a

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针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗USP9抗体(5G-02)在各种科学出版物中有4篇引文。

针对哺乳动物靶标蛋白的一抗，如抗USP9 抗体 (5G-02)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

USP9 抗体 (5G-02) Specifications

替代产品名称

USP9

产品克隆名称

5G-02

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG₁ kappa light chain

氨基酸

1-91

抗原表位靶标

USP9

抗原表位种属

human

产品反应性/检测

USP9

种属反应性/检测

human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, IF, ELISA

瓶的信息

Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

4篇各种科学出版物的引文

可选的偶联物/形式

N/A

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗USP9抗体(5G-02)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

A double band around 290K is detected using USP9 antibody in Western blot. (Double bands are also seen in the sample image as well.) Which one is the band of USP9? Also, if one is a non-specific band, can I buy a blocking peptide for USP9?

Asked by: Nori

Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.

Answered by: Technical Support

Date published: 2021-07-23

I am using USP9 (5G-02): sc-100628 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Germaine

Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining

Answered by: Technical Support

Date published: 2017-03-24

Rated 5 out of 5 by SCBT QC Lab from Great for Western Blot Antibody has been used to detect USP9 in epidermoid carcinoma cell line AW8507 and squamous cell carcinoma cell line AW13516 and SCC029B by western blot in published research. -SCBT QC

Date published: 2023-09-14

Rated 3 out of 5 by SH from Good specificity in WB in HeLa nuclear extract Good specificity in WB in HeLa nuclear extract. -SCBT QC

Date published: 2015-05-09

Rated 5 out of 5 by TS from Published data for WB in HEK293T cells Published data for WB in HEK293T cells. -SCBT Publication Review

Date published: 2015-04-25

Rated 3 out of 5 by HC from Decent immunofluorescence cytoplasmic staining Decent immunofluorescence cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC

Date published: 2015-01-13

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USP9 抗体 (5G-02): sc-100628

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References:

USP9 抗体 (5G-02) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

A double band around 290K is detected using USP9 antibody in Western blot. (Double bands are also seen in the sample image as well.) Which one is the band of USP9? Also, if one is a non-specific band, can I buy a blocking peptide for USP9?

I am using USP9 (5G-02): sc-100628 for immunofluorescence, what dilution of this antibody should I use?