Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
USP9 Antibody (5G-02) is a high quality monoclonal USP9 antibody (also designated USP9X antibody) suitable for the detection of the USP9 protein of human origin. USP9 Antibody (5G-02) is available as the non-conjugated anti-USP9 antibody. The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP9 (ubiquitin specific peptidase 9), also known as USP9X, FAF or DFFRX, is a 2547 amino acid member of the peptidase C19 family of ubiquitin proteases. Expressed ubiquitously in both fetal and adult tissue, USP9 is involved in the processing of ubiquitin precursors and ubiquitinated proteins, thereby preventing degradation and regulating protein turnover. Defects in the gene encoding USP9 are implicated in Turner syndrome, a condition in which oocytes fail to proliferate and develop, leading to the degeneration of the developing ovary. Multiple isoforms encoding long and short transcripts exist due to alternative splicing events.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I am using USP9 (5G-02): sc-100628 for immunofluorescence, what dilution of this antibody should I use?
Asked by: Germaine
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here:
Answered by: Technical Support
Date published: 2017-03-24
A double band around 290K is detected using USP9 antibody in Western blot.
(Double bands are also seen in the sample image as well.)
Which one is the band of USP9?
Also, if one is a non-specific band, can I buy a blocking peptide for USP9?