USP34抗体 (3H9) 推荐用于 human 来源的USP34 WB, IP, IF, IHC(P) 和 ELISA检测

USP34 抗体 (3H9): sc-100631

USP34抗体 (3H9) is rated 5.0 out of 5 by 3.
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Datasheets

    • 抗-USP34 抗体 3H9 是小鼠单克隆 IgG2a (kappa轻链),USP34抗体,规格为100 µg/ml
    • 免疫human物种的重组USP34
    • 推荐用于 human 来源的USP34 WB, IP, IF, IHC(P) 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取USP34 (3H9): sc-100631免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
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What percentage gel/ recommended transfer conditions do you suggest for a good band for USP34? I have used recommended dilution of 1:200 and see multiple bands (of similar intensity) above 250kd MW marker band.

Asked by: SMahajan
Thank you for your question. This is a very large protein, so we recommend a 8% gel for this protein. Please contact our Technical Service Department if you need further assistance with this antibody.
Answered by: Tech Service
Date published: 2019-02-28

What peptide has been used as the immunogen for this antibody?

Asked by: lms104
Thank you for your question. USP34 (3H9): sc-100631 was raised against amino acids 2396-3396 of human recombinant USP34 (accession# Q70CQ2). Please contact our technical service directly for additional information.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Can sc-100631: USP34 (3H9) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, sc-100631: USP34 (3H9) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from Produced beautiful immunoperoxidase nuclear Produced beautiful immunoperoxidase nuclear staining in formalin-fixed, paraffin-embedded human seminoma tissue. -SCBT QC
Date published: 2015-04-17
Rated 5 out of 5 by from Positive band seen in WB in A549 whole cell lysate Positive band seen in WB in A549 whole cell lysate. -SCBT QC
Date published: 2015-04-14
Rated 5 out of 5 by from Strong band detected in WB in HeLa whole Strong band detected in WB in HeLa whole cell lysate. -SCBT QC
Date published: 2015-01-27
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