Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
USP34 Antibody (3H9) is a high quality monoclonal USP34 antibody (also designated USP34 antibody) suitable for the detection of the USP34 protein of human origin. USP34 Antibody (3H9) is available as the non-conjugated anti-USP34 antibody. The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP34 (ubiquitin specific peptidase 34) is a 3,395 amino acid protein that belongs to the peptidase C19 family of proteins. Expressed at low levels in brain, USP34 functions as a deubiquitinating enzyme that cleaves ubiquitin residues from both ubiquitinylated proteins and ubiquitin-fused precursors, thereby saving these proteins from proteasomal degradation. In response to DNA damage, USP34 is phosphorylated by ATM or ATR. Two isoforms of USP34 are expressed due to alternative splicing events.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
What percentage gel/ recommended transfer conditions do you suggest for a good band for USP34? I have used recommended dilution of 1:200 and see multiple bands (of similar intensity) above 250kd MW marker band.
Asked by: SMahajan
Thank you for your question. This is a very large protein, so we recommend a 8% gel for this protein. Please contact our Technical Service Department if you need further assistance with this antibody.
Answered by: Tech Service
Date published: 2019-02-28
What peptide has been used as the immunogen for this antibody?
Asked by: lms104
Thank you for your question. USP34 (3H9): sc-100631 was raised against amino acids 2396-3396 of human recombinant USP34 (accession# Q70CQ2). Please contact our technical service directly for additional information.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06
Can sc-100631: USP34 (3H9) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?
Asked by: Trav11
Thank you for your question. Yes, sc-100631: USP34 (3H9) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here:
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