USP33 抗体 (1D7): sc-100632

USP33 Antibody (1D7) is an IgG₁ κ mouse monoclonal USP33 antibody that detects the USP33 protein of human origin by WB, IP, IF, IHC(P) and ELISA. USP33 Antibody (1D7) is available as the non-conjugated anti-USP33 antibody. The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP33 (ubiquitin specific peptidase 33), also known as VDU1 (VHL-interacting deubiquitinating enzyme 1), is a widely expressed 942 amino acid protein that belongs to the peptidase C19 family of proteins. Containing two DUSP domains and a UBP-type zinc finger, USP33 functions as deubiquitinating enzyme that cleaves ubiquitin residues from both ubiquitinylated proteins and ubiquitin-fused precursors, thereby saving these proteins from proteasomal degradation. In addition, USP33 binds VHL and can be ubiquitinated for degradation in a VHL-dependent manner. Three isoforms of USP33 are expressed due to alternative splicing events.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. Ubiquitination of a novel deubiquitinating enzyme requires direct binding to von Hippel-Lindau tumor suppressor protein.  |  Li, Z., et al. 2002. J Biol Chem. 277: 4656-62. PMID: 11739384
2. Identification of a deubiquitinating enzyme subfamily as substrates of the von Hippel-Lindau tumor suppressor.  |  Li, Z., et al. 2002. Biochem Biophys Res Commun. 294: 700-9. PMID: 12056827
3. Human and mouse proteases: a comparative genomic approach.  |  Puente, XS., et al. 2003. Nat Rev Genet. 4: 544-58. PMID: 12838346
4. Deubiquitination of type 2 iodothyronine deiodinase by von Hippel-Lindau protein-interacting deubiquitinating enzymes regulates thyroid hormone activation.  |  Curcio-Morelli, C., et al. 2003. J Clin Invest. 112: 189-96. PMID: 12865408
5. Triplets! Unexpected structural similarity among the three enzymes that catalyze initiation and termination of thyroid hormone effects.  |  Bianco, AC. 2004. Arq Bras Endocrinol Metabol. 48: 16-24. PMID: 15611815
6. VHL protein-interacting deubiquitinating enzyme 2 deubiquitinates and stabilizes HIF-1alpha.  |  Li, Z., et al. 2005. EMBO Rep. 6: 373-8. PMID: 15776016
7. A leukemia-enriched cDNA microarray platform identifies new transcripts with relevance to the biology of pediatric acute lymphoblastic leukemia.  |  De Pittà, C., et al. 2005. Haematologica. 90: 890-8. PMID: 15996926
8. Cellular and structural biology of the deiodinases.  |  Bianco, AC. and Larsen, PR. 2005. Thyroid. 15: 777-86. PMID: 16131321
9. The solution structure of the ZnF UBP domain of USP33/VDU1.  |  Allen, MD. and Bycroft, M. 2007. Protein Sci. 16: 2072-5. PMID: 17766394