UBE2T 抗体 (4E5): sc-100623

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- UBE2T 抗体 4E5 是小鼠单克隆 IgG_2b (kappa轻链)，UBE2T抗体，规格为100 µg/ml
- 免疫human物种的重组UBE2T
- 推荐用于 human 来源的UBE2T WB, IP 和 ELISA检测
- 关于如何获取UBE2T (4E5): sc-100623的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
- 目前，我们还没有完成UBE2T 抗体 (4E5)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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此外 UBE 抗体包括 UBE1, UBE1L, UBE2A, UBE2B, UBE2C, UBE2D, UBE2D2, UBE2E1, UBE2F和 UBE2G1
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UBE2T Antibody (4E5) - Western Blotting - Image 33994

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UBE2T Antibody (4E5) is an IgG_2b κ mouse monoclonal UBE2T antibody (also designated UBE2T antibody) suitable for the detection of the UBE2T protein of human origin by WB, IP and ELISA. UBE2T Antibody (4E5) is available as the non-conjugated anti-UBE2T antibody. Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). UBE2T (Ubiquitin-conjugating enzyme E2 T), also known as PIG50 or HSPC150, is a 197 amino acid member of the E2 ubiquitin-conjugating enzyme family. Involved in the protein degradation pathway, UBE2T catalyzes the ATP-dependent attachment of ubiquitin (Ub) to target proteins, thereby tagging them for subsequent destruction by the proteasome. Additionally, UBE2T is thought to be a crucial component of the Faconi anemia pathway of DNA damage repair and, upon self-inactivation, may negatively regulate the Faconi pathway.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

ATR couples FANCD2 monoubiquitination to the DNA-damage response. | Andreassen, PR., et al. 2004. Genes Dev. 18: 1958-63. PMID: 15314022
UBE2T is the E2 in the Fanconi anemia pathway and undergoes negative autoregulation. | Machida, YJ., et al. 2006. Mol Cell. 23: 589-96. PMID: 16916645
Fanconi anemia and ubiquitination. | Zhang, Y., et al. 2007. J Genet Genomics. 34: 573-80. PMID: 17643942
UBE2T, the Fanconi anemia core complex, and FANCD2 are recruited independently to chromatin: a basis for the regulation of FANCD2 monoubiquitination. | Alpi, A., et al. 2007. Mol Cell Biol. 27: 8421-30. PMID: 17938197
FANCD2 monoubiquitination provides a link between the HHR6 and FA-BRCA pathways. | Zhang, J., et al. 2008. Cell Cycle. 7: 407-13. PMID: 18277096

种属	基因名称	基因ID	染色质位置	亚型（mRNA）即位号码	蛋白质即位号码	OMIM™ 号码
Human	UBE2T	Entrez Gene ID: 29089	1q32.1	NM_001310326, NM_014176	UniProt ID: Q9NPD8	610538

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针对哺乳动物和非哺乳动物蛋白靶点的单克隆抗体基本上代表了多克隆抗体覆盖的所有靶点。我们提供超过8300种ImmunoCruz^®偶联抗体，从而可以省去使用二抗。

圣克鲁斯生物技术的抗体有超过36万的研究引文。抗UBE2T抗体(4E5)在各种科学出版物中有0篇引文。

针对哺乳动物靶标蛋白的一抗，如抗UBE2T 抗体 (4E5)，推荐用于检测一系列哺乳动物物种，主要有小鼠，大鼠和人物种。许多哺乳动物抗体也非常适合兽医研究，与羊、猪、羊、猫、犬、牛和马的蛋白靶点反应。圣克鲁斯生物技术的单克隆一抗适用于实验室研究应用，不用于诊断或治疗。

UBE2T 抗体 (4E5) Specifications

替代产品名称

UBE2T

产品克隆名称

4E5

分类

一抗

Clonality

单克隆抗体

抗体同型

IgG_2b kappa light chain

氨基酸

1-198

抗原表位靶标

UBE2T

抗原表位种属

human

产品反应性/检测

UBE2T

种属反应性/检测

human

其它哺乳动物反应性

N/A

其它非哺乳动物反应性

N/A

应用

WB, IP, ELISA

瓶的信息

Each vial contains 100 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文

0篇各种科学出版物的引文

可选的偶联物/形式

N/A

针对非哺乳动物靶点的一抗在检测植物、病毒、细菌、鸟类、鱼类、酵母和无脊椎动物的蛋白质方面非常有用。例如果蝇,斑马鱼, 非洲爪蟾蜍, 拟南芥, 秀丽隐杆线虫, 梭菌, 弯曲杆菌, 幽门螺杆菌, 肝炎, HIV, HPV, HSV, 流行性感冒, 克雷白氏杆菌, 结核分支杆菌, 疟原虫, 假单胞菌, RSV, 酿酒酵母, 沙门氏菌, 血吸虫, 志贺氏杆菌 和 葡萄球菌.

圣克鲁斯生物技术单克隆抗体可用于流式细胞术、免疫沉淀、Western blot和免疫染色等分析。抗UBE2T抗体(4E5)推荐用于以下应用：WB (Western blotting), IP (Immunoprecipitation), ELISA (Enzyme-Linked Immunosorbent Assay).

我们还有小鼠二抗、对照血清和对照免疫球蛋白，可与我们的单克隆一抗联合使用。

组织提取物，核提取物和全细胞裂解物与圣克鲁斯生物技术单克隆抗体使用，作为western blotting或免疫沉淀阳性对照。核提取物是理想的western blot分析或凝胶移位分析阳性对照。磷酸化富集的全细胞裂解液和组织提取物可用于磷酸化特异性抗体阳性对照。

单克隆二抗有多种偶联形式，包括：Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), 和TRITC (tetramethyl rhodamine isothiocyanate) (R)，用于研究应用，像流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC）染色和蛋白印迹（WB）。不用于诊断或治疗。

偶联和非偶联的亲和纯化的免疫球蛋白(IgGs)，用于流式细胞术（FCM），免疫荧光（IF），免疫组织化学（IHC），免疫沉淀（IP）和蛋白印迹（WB）应用，作为阴性对照。

正常血清用于免疫组织化学（IHC）应用，作为封闭试剂。所使用的血清应与二抗的种属相匹配。

For Western Blot, is it recommended to use denatured or non-denatured conditions with UBE2T (4E5): sc-100623 antibody?

Asked by: Trav11

Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.

Answered by: Technical Support

Date published: 2017-03-27

Rated 5 out of 5 by hangyin from 抗体完美，赞一个抗体不错，赞一个，就是时间有点长，没有现货，说明书很好，抗体很好，服务很好，总之很好。very good

Date published: 2017-11-20

Rated 5 out of 5 by Lyli from Great antibody for western blot We brought this antibody one month ago. This antibody is great for western blot. We got nice UBE2T band.

Date published: 2017-07-31

Rated 4 out of 5 by LR from Clear band of interest seen in WB in HeLa Clear band of interest seen in WB in HeLa whole cell lysate. -SCBT QC

Date published: 2013-12-10

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UBE2T 抗体 (4E5): sc-100623

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References:

UBE2T 抗体 (4E5) Specifications

替代产品名称

产品克隆名称

分类

Clonality

抗体同型

氨基酸

抗原表位靶标

抗原表位种属

产品反应性/检测

种属反应性/检测

其它哺乳动物反应性

其它非哺乳动物反应性

应用

瓶的信息

研究引文

推荐的二抗检测试剂

可选的偶联物/形式

For Western Blot, is it recommended to use denatured or non-denatured conditions with UBE2T (4E5): sc-100623 antibody?