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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
UBE2D2 Antibody (Y-20) is a high quality monoclonal UBE2D2 antibody (also designated UBE2D2 antibody) suitable for the detection of the UBE2D2 protein of mouse, rat and human origin. UBE2D2 Antibody (Y-20) is available as the non-conjugated anti-UBE2D2 antibody. Ubiquitin is an abundant, highly conserved protein found in all eukaryotic cells either free or covalently attached to cellular proteins. The primary function of ubiquitin in mammalian systems is to clear abnormal, foreign, and improperly folded proteins by targeting them for proteosome degradation. In Saccharomyces cerevisiae, ubiquitin-like proteins include Rub1, Ula1, Uba3, Smt3, Ubc2, Ubc12 and Ubc9. Rub1 shares 53% homology with ubiquitin and requires activation via the E2 proteins, including Ula1, Uba3 and Ubc12, in order to conjugate to substrates directed to different proteolytic systems. UBE2D2 catalyzes ubiquitination of IkBa in a phosphorylation and SCFB-TRCP dependent manner. In this particular reaction, E1 first transfers ubiquitin to the E2 component UBE2D2, and UBE2D2 then associates with E3 ligase, which conjugates the poly-ubiquitin chain on a target protein. In this fashion, the chain tags the IkBa for degradation by a proteasome thus lifting the inhibitory effect of IkBa on NFkB and allowing NFkB to enter the nucleus.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Can sc-100617: UBE2D2 (Y-20) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?
Asked by: TinTin
Thank you for your question. Yes, sc-100617: UBE2D2 (Y-20) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here:
Rated 5 out of
Strong positive band observed in WB in mouseStrong positive band observed in WB in mouse UBE2D2 transfected 293T whole cell lysate and rat testis tissue extract. -SCBT QC
Date published: 2015-06-28
Rated 5 out of
Produced excellent immunoperoxidase cytoplasmicProduced excellent immunoperoxidase cytoplasmic staining in formalin-fixed, paraffin-embedded human small Intestine tissue. -SCBT QC
Date published: 2015-04-02
Rated 5 out of
ClearClear, specific band obtained in WB in NIH/3T3 whole cell lysate. -SCBT QC