TSC-22抗体 (R5-6) 推荐用于 mouse, rat 和 human 来源的TSC-22 WB, IP, IF 和 ELISA检测

TSC-22 抗体 (R5-6): sc-101195

TSC-22抗体 (R5-6) is rated 4.0 out of 5 by 2.
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Datasheets

    • 抗-TSC-22 抗体 R5-6 是小鼠单克隆 IgG1 (kappa轻链),TSC-22抗体,规格为100 µg/ml
    • 免疫human物种的重组TSC-22
    • 推荐用于 mouse, rat 和 human 来源的TSC-22 WB, IP, IF 和 ELISA检测
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取TSC-22 (R5-6): sc-101195免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

Hello. I want to know the epitope of TSC-22 to which the sc-101195 antibody binds. Can you tell us the exact location?

Asked by: pico750
Thank you for your question. TSC-22 Antibody (R5-6): sc-101195 has been raised against a recombinant human TSC-22 construct containing the N-terminal amino acids 1-145 of TSC-22. The exact epitope sequence has not been mapped yet.
Answered by: Tech Support Europe
Date published: 2020-07-07

We are interested in the identification of TSC22D1 expression in mouse fibroblasts and liver samples. The antibody "TSC-22 Ab (R5-6): sc-101195" refers to TSC22D1 or any of the TSC22D1-4 proteins identified so far are reacting with this SCBT antibody?

Asked by: Hagi10
Thank you for your question. TSC-22 Antibody (R5-6): sc-101195 has been raised against recombinant TSC-22 of human origin (encoded by the human TSC22D1 gene). There is little homology between TSC22D1 and TSC22D2-4, so it is unlikely that the antibody recognizes these proteins as well. Please contact our technical service directly for further information or to arrange for as sample of the antibody to test it in your application.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

I am using TSC-22 (R5-6): sc-101195 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jenni
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Good immunofluorescence nuclear and cytoplasmic Good immunofluorescence nuclear and cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-02-14
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